Spermatic Parameters Clinical Trial
Official title:
Impact of Nuclear Quality of Human Sperm on Embryonic Development Kinetics Evaluated by Time Lapse Primovision®
To date, none study shows the impact of human spermatozoa nuclear alteration on embryonic development kinetic with morpho-kinetics tools. In this study, Investigator analyze the possible influence of sperm nuclear quality on embryonic development kinetics. Moreover, Investigator will evaluate possible new sperm biomarkers and try to better understand the pathophysiology of male infertility.
In recent years, there has clearly been a significant decline in male fertility in
industrialized countries, particularly sperm quality. Furthermore, it is known that the
quality of sperm DNA affects embryonic development and pregnancy outcomes. However, few
elements are known about the effects of spermatozoa nuclear alterations on the embryonic
development kinetics.
Thus, the aim of this study is to analyze the possible influence of sperm nuclear quality on
the embryonic development kinetics. In order to answer this question, Investigator will study
the spermatozoa quality of patients whose torque is supported by ICSI.
On these spermatozoa, Investigator will analyze DNA fragmentation, oxidation by measuring the
8-OHdG residues, chromatin compaction and nuclear methylation degree. This will allow to
determine the spermatic nuclear parameters in relation to an ICSI fertilization rate (normal>
60%) and a good blastocyst rate (≥ B3 in the Gardner classification).
And finally, by this study, Investigator will probably find new biological markers spermatic.
Moreover, the data will help to better understand the physiopathology of male infertility.
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| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT03734120 -
Cryogenic Preservation of Spermatozoa
|