The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

Smoking Clinical Trial

Official title:

The Effect of Smoking and Non-surgical Periodontal Treatment on Gingival Crevicular Fluid Cyclophilin A and EMMPRIN Levels

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04430764
• Other study ID #: 2014/Dis/019
• Status: Completed
• Phase: N/A
• Start date: October 2014
• Est. completion date: August 2015

Study information

• Verified date: June 2020
• Source: Ege University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study aimed to investigate the effect of smoking and non-surgical periodontal treatment on gingival crevicular fluid CyPA and EMMPRIN levels in patients with periodontitis. Twenty-eight patients with periodontitis (14 non-smoker and 14 smoker) and 30 control subjects (15 non-smoker and 15 smoker) were included into the study. Non-surgical periodontal treatment was applied to all periodontitis patients. Periodontal parameters and GCF CyPA and EMMPRIN levels were evaluated during 3 months following non-surgical periodontal treatment. GCF CyPA and EMMPRIN levels were analyzed by ELISA.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 58
• Est. completion date: August 2015
• Est. primary completion date: August 2015
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 29 Years to 61 Years

Eligibility

Inclusion Criteria:

• generalized periodontitis with stage III-IV & Grade B

• no systemic and periodontal diseases

• smokers (minimum 10 cigarettes per day for at least ten years)

• non-smokers (never smoked)

Exclusion Criteria:

• Systemic diseases

• Infectious diseases

• Pregnancy or lactating

• Having periodontal treatment during the last 6 months

• Having antibiotic treatment within the past 3 months

• Former smokers

Related Conditions & MeSH terms

• Non-surgical Periodontal Treatment
• Periodontitis
• Smoking

Intervention

Procedure:
• non-surgical periodontal treatment
Non-surgical periodontal treatment program was as following: First, motivation and oral hygiene instructions were given to all participants with periodontitis. The modified-Bass tooth brushing technique with a given dentifrice and toothbrush twice a day, and dental flossing/ interdental brushing once a day was advised to all patients with periodontitis. Second, scaling using an ultrasonic device and root planning by hand instruments under local anesthesia was performed.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Ege University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in gingival crevicular fluid CypA total amounts	GCF CyPA levels were analyzed by ELISA	Baseline, 1 week, 1., 3. months
Primary	Change in gingival crevicular fluid EMMPRIN total amounts	GCF EMMPRIN levels were analyzed by ELISA	Baseline, 1 week, 1., 3. months
Secondary	Change in probing depth	Probing depth was measured with Williams periodontal probe.	Baseline, 1., 3. months
Secondary	Change in bleeding on probing	Bleeding on probing was evaluated the presence or absense following bleeding upon probing.	Baseline, 1., 3. months