Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis

Smoking Clinical Trial

Official title:

The Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis: A Cross-sectional Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03426241
• Other study ID #: BaskentU2
• Status: Completed
• Phase: N/A
• First received: January 29, 2018
• Last updated: February 7, 2018
• Start date: February 10, 2010
• Est. completion date: December 30, 2012

Study information

• Verified date: February 2018
• Source: Baskent University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

This study comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the hypothesis of this study was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Description:

Smokers have greater risk for developing periodontal disease and smoking is highly correlated with greater marginal bone loss. Matrix metalloproteinases (MMP) play a major role in periodontal tissue destruction. MMP-2 and MMP-9, called gelatinases, are one of the major MMPs that plays important roles in periodontitis. To date, several studies showed the increased levels of gelatinases in periodontal disease and only a limited number of study investigated the effect of smoking on gelatinases. However, no previous study has directly investigated the possible relationship between smoking and gingival tissue gelatinases expressions (MMP-2 and MMP-9) in chronic periodontitis (CP) patients relative to periodontally healthy subjects.

Therefore this study is conducted to comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the of this study hypothesis was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 80
• Est. completion date: December 30, 2012
• Est. primary completion date: December 29, 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 16 Years to 62 Years

Eligibility

Inclusion Criteria:

• A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry between February 2010 and December 2012.

Exclusion Criteria:

• Patients with systemic disease;

• pregnant and/or lactating women;

• patients taking any medications that may influence the study.

Related Conditions & MeSH terms

• Chronic Periodontitis
• Periodontitis
• Smoking

Intervention

Other:
• no intervention
this is cross-sectional study

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Baskent University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	expression of gelatinases levels in gingival tissue among groups	In chronic periodontitis group, after recording the clinical parameters, gingival tissues were harvested from the deepest periodontal pocket. Whereas in periodontally healthy group, gingival tissues were collected during crown-lengthening surgery or during an extraction of a teeth for orthodontic reasons. In both groups the samples include both sulcus/pocket epithelium and underlying connective tissues. The expression of MMP-2 and MMP-9 is measured immunohistochemically	day 1
Secondary	difference in probing depths among groups	Clinical parameters will be recorded and difference in probing depths among the groups will be calculated	day o
Secondary	difference in probing depths among groups	Clinical parameters will be recorded and difference in clinical attahment level among the groups will be calculated	day 0