To Determine the Activity and Mechanisms of Cranberries to Attenuate Skin Photoaging and Improve Skin Health in Women

Skin Health Clinical Trial

Official title:

To Determine the Activity and Mechanisms of Cranberries to Attenuate Skin Photoaging and Improve Skin Health in Women

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT04183920
• Other study ID #: IRB201903250
• Status: Recruiting
• Phase: N/A
• Start date: February 7, 2020
• Est. completion date: February 3, 2026

Study information

• Verified date: June 2023
• Source: University of Florida
• Contact: Lindsey Morgan Christman
• Phone: 352-294-3731
• Email: lchristman[@]ufl.edu
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Previous studies have shown that cranberry phytochemicals scavenge reactive carbonyls. Cranberry polyphenols have been reported to attenuate collagen degradation and inhibit oxidative stress in human cells. We hypothesize that cranberry juice consumption will attenuate skin photoaging and improve skin health in women via alleviate oxidative stress.

Description:

One of the causative factors of skin ageing is cumulative oxidative damages due to aerobic metabolism and UV irradiation. Cumulation of reactive oxygen species (ROS) causes collagen glycation and crosslinking which are direct causes of skin ageing. Previous studies have shown that cranberry phytochemicals scavenge reactive carbonyls. Cranberry polyphenols have been reported to attenuate collagen degradation and inhibit oxidative stress in human cells. It is unknown whether cranberry juice consumption could protect against UV -induced erythema and improve overall skin health. Furthermore, if it does have effect, what will be the mechanism? This human intervention study will be conducted to answer these questions. We hypothesize that cranberry juice consumption will attenuate skin photoaging and improve skin health in women via alleviate oxidative stress.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 22
• Est. completion date: February 3, 2026
• Est. primary completion date: February 3, 2026
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Female
• Age group: 21 Years to 65 Years

Eligibility

Inclusion Criteria:

• Healthy
• BMI (18.5-29.9)
• Body weight =110 pounds

Exclusion Criteria:

• Skin Cancer
• Smokers and frequent alcohol use
• Pregnancy
• Breast-feeding
• intake of medication that might influence the outcome of the study
• sunbathing or the use of tanning bed
• intake of vitamin/mineral supplements

Related Conditions & MeSH terms

• Skin Health

Intervention

Other:
• Cranberry juice
cranberry juice cocktail provided by Ocean Spray Cranberries, Inc.
• Placebo juice
apple juice added with flavor and colorants provided by Ocean Spray Cranberries, Inc.

Locations

Country	Name	City	State
United States	Food Science and human nutrition department at University of Florida	Gainesville	Florida

Sponsors (2)

Lead Sponsor	Collaborator
University of Florida	Ocean Spray, Inc.

Country where clinical trial is conducted

United States, 

References & Publications (4)

Heinrich U, Moore CE, De Spirt S, Tronnier H, Stahl W. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women. J Nutr. 2011 Jun;141(6):1202-8. doi: 10.3945/jn.110.136465. Epub 2011 Apr 27. — View Citation

Heinrich U, Neukam K, Tronnier H, Sies H, Stahl W. Long-term ingestion of high flavanol cocoa provides photoprotection against UV-induced erythema and improves skin condition in women. J Nutr. 2006 Jun;136(6):1565-9. doi: 10.1093/jn/136.6.1565. — View Citation

Liu H, Liu H, Wang W, Khoo C, Taylor J, Gu L. Cranberry phytochemicals inhibit glycation of human hemoglobin and serum albumin by scavenging reactive carbonyls. Food Funct. 2011 Aug;2(8):475-82. doi: 10.1039/c1fo10087d. Epub 2011 Aug 8. — View Citation

Ramasamy R, Yan SF, Schmidt AM. Receptor for AGE (RAGE): signaling mechanisms in the pathogenesis of diabetes and its complications. Ann N Y Acad Sci. 2011 Dec;1243:88-102. doi: 10.1111/j.1749-6632.2011.06320.x. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change from baseline Skin color change after UV irradiation at 28 days	Irradiation will be applied to dorsal skin (region not typically exposed to the sun) using an FDA approved UVB phototherapy light and a UV light meter. At each assessment, skin color will be measured before and 24 h after irradiation. Skin color will be evaluated by a colorimeter using the 3-dimensional color system with L-, a-, and b-values. L- and b- values assess lightness and browning effects, respectively. The a-value (red/green-axis) is a measure for reddening (erythema). The ?a-values (a-value 24 h after irradiation minus a-value before irradiation) will compared between baseline through 3 months; decreasing ?a-values indicates a photoprotective effect.	Baseline, 28 days
Primary	Change from baseline Skin transepidermal water loss	Skin transepidermal water loss an will be measured using a Tewameter (CK Electronic GmbH, Germany). This is a handheld probes that allow for quick and easy measurements	Baseline, 28 days
Primary	Change from baseline SELS parameters	SELS (Surface evaluation of the Living Skin) parameters, including smoothness, roughness, scaliness, and wrinkles, will be determined using a Visioscan VC20. This hand-hold device scans a 1 cm x 1 cm skin area (https://www.courage-khazaka.de/en/scientific-products/all-products/imaging/16-wissenschaftliche-produkte/alle-produkte/150-visioscan-e). Visioscan VC20 scanner consists of a camera, a high- resolution video sensor, and a UVA light no hazardous to human skin.	Baseline, 28 days
Primary	Change in skin microbiome	For skin swabbing, a 4x4-cm square on a forearm will be swabbed with a cotton swab soaked in 0.9% sodium chloride with 0.1% Tween-20 in a Z-stroke manner.	Baseline, 28 days
Primary	Change in baseline skin pH	Skin pH will be assessed using a skin pH meter (CK Electronic GmbH, Germany). This is a handheld probes that allow for quick and easy measurements.	Baseline, 28 days
Primary	Change from baseline hydration	Skin hydration will be measured with a Corneometer (CK Electronic GmbH, Germany. This is a handheld probe that allows for quick and easy measurements.	Baseline, 28 days
Primary	Change in Skin erythema and melanin index	Skin erythema and melanin index will be assessed with a Mexameter, (CK Electronic GmbH, Germany). Each of these are handheld probes that allow for quick and easy measurements	Baseline, 28 days
Primary	Change in skin lipidome	Stratum corneum layer on forearm skin will be sampled with 16 D-squame® tapes using a standard noninvasive tape stripping technique. This will be sent for lipidomic analysis	baseline, 28 days
Secondary	Survival rate of human keratinocyte under UV light	The immortalized human keratinocyte cell line (HaCaT) will be cultured in Dulbecco's Modified Eagle Medium. Cells will be incubated with human serum collected after 2-hour and 4-hour cranberry juice intake or water intake for 24 hours.	24 hours