Skeletal Muscle Energetics Clinical Trial
Official title:
Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle
| Verified date | May 2019 |
| Source | Queen's University |
| Contact | n/a |
| Is FDA regulated | No |
| Health authority | |
| Study type | Interventional |
This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.
| Status | Completed |
| Enrollment | 10 |
| Est. completion date | April 30, 2019 |
| Est. primary completion date | April 30, 2019 |
| Accepts healthy volunteers | Accepts Healthy Volunteers |
| Gender | Male |
| Age group | 18 Years to 30 Years |
| Eligibility |
Inclusion Criteria: - recreationally active (no more than 3 hours of weekly physical activity) - not currently involved in a systematic training program - body mass index < 30 kg/m2 Exclusion Criteria: - presence of cardiometabolic disease - taking regular oral medication - current smoker |
| Country | Name | City | State |
|---|---|---|---|
| Canada | School of Kinesiology and Health Studies | Kingston | Ontario |
| Lead Sponsor | Collaborator |
|---|---|
| Queen's University | University of Waterloo |
Canada,
| Type | Measure | Description | Time frame | Safety issue |
|---|---|---|---|---|
| Primary | Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1a) messenger ribonucleic acid (mRNA) expression | PGC-1a gene expression (indirect indicator of PGC-1a activity) | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Catecholamine concentrations | Epinephrine and norepinephrine concentrations in plasma samples | 15 min before fast; after exercise; after 4 and 8 hours of fasting | |
| Secondary | Glucose and insulin concentrations | Glucose and insulin concentrations in plasma samples | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated AMP activated protein kinase (AMPK) | active AMPK relative to total AMPK protein content | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated SIRT-1 | active SIRT-1 relative to total SIRT1 protein content | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated mammalian target of rapamycin (mTOR) | active mTOR relative to total mTOR protein content | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated Akt/Protein Kinase B | active Akt relative to total Akt protein content | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated acetyl coenzyme A carboxylase (ACC) | active ACC relative to total ACC protein | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | total and phosphorylated p38 mitogen activated protein kinase (MAPK) | active p38 MAPK relative to total p38 MAPK protein | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | phosphorylated protein kinase A (PKA) substrates | phosphorylated PKA substrates (indirect measure of PKA activity) | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | acetylated p53 | p53 acetylation status (indirect measure of SIRT-1 deacetylase activity) | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Sirtuin 1 (SIRT1) mRNA expression | gene expression of the deacetylase enzyme SIRT1 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Sirtuin 3 (SIRT3) mRNA expression | gene expression of the deacetylase enzyme SIRT3 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression | gene expression of the PGC-1a target PDK4 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | p53 mRNA expression | gene expression of the tumour suppressor protein p53 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression | gene expression of RNA-binding protein LRP130 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | General control non-repressible 5 (GCN5) mRNA expression | gene expression of the acetyltransferase GCN5 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | PGC-1a protein content | Protein content of the transcriptional co-activator PGC-1a | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Cytochrome C oxidase subunit 4 (COX-IV) protein content | Protein content of the mitochondrial enzyme COX subunit 4 | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Intramuscular glycogen content | Muscle glycogen content | 15 min before fast; after 4 and 8 hours of fasting | |
| Secondary | Intramuscular triglyceride (IMTG) content | Muscle IMTG content | 15 min before fast; after 4 and 8 hours of fasting |