Metabolism of Branched-chain Amino Acids in Monocyte/Macrophage During Sepsis

Sepsis Syndrome Clinical Trial

Official title:

Metabolism of Branched-chain Amino Acids in Monocyte and Macrophage During Sepsis

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT03842371
• Other study ID #: 2018-081
• Status: Not yet recruiting
• Start date: February 11, 2019
• Est. completion date: February 28, 2022

Study information

• Verified date: February 2019
• Source: West China Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Description:

In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 90
• Est. completion date: February 28, 2022
• Est. primary completion date: December 30, 2021
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 70 Years

Eligibility

Inclusion Criteria:

• patients diagnosed with sepsis, with or without type 2 diabetes

• healthy volunteers

Exclusion Criteria:

• patients with immunodeficient diseases

• patients who accept glucocorticoid or immunosuppressant

• patients with an age younger than 18 years

• patients who cannot provide the informed consent

Related Conditions & MeSH terms

• Sepsis
• Sepsis Syndrome
• Systemic Inflammatory Response Syndrome
• Toxemia

Intervention

Other:
• Metabolic level of BCAAs in monocytes and macrophages
Metabolic level of BCAAs in monocytes and macrophages

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
West China Hospital

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Intracellular levels of branched-chain amino acids in monocytes	BCAAs Assay Kit will be used to measure the intracellular levels of BCAAs in monocytes.	up to 14 day after diagnose of sepsis
Secondary	Plasma levels of branched-chain amino acids	BCAAs Assay Kit will be used to measure the plasma levels of BCAAs.	1,3,5,7,10,14 day after diagnose of sepsis
Secondary	The expression of branched-chain amino acid aminotransferase in monocytes	The expression of branched-chain amino acid aminotransferase in monocytes will be determined by qPCR and western blot.	1,3,5,7,10,14 day after diagnose of sepsis
Secondary	The expression of branched-chain a-ketoacid dehydrogenase in monocytes	The expression of branched-chain a-ketoacid dehydrogenase in monocytes will be determined by qPCR and western blot.	1,3,5,7,10,14 day after diagnose of sepsis
Secondary	The expression of PP2Cm in monocytes	The expression of mitochondrial-targeted phosphatase, PP2Cm in monocytes will be determined by qPCR and western blot.	1,3,5,7,10,14 day after diagnose of sepsis
Secondary	The plasma level of IL-1ß	The plasma level of IL-1ß will be determined by ELISA kit.	1,3,5,7,10,14 day after diagnose of sepsis.
Secondary	The plasma level of TNF-a	The plasma level of TNF-a will be determined by ELISA kit.	1,3,5,7,10,14 day after diagnose of sepsis.
Secondary	The plasma level of IL-10	The level of IL-10 will be determined by ELISA kit.	1,3,5,7,10,14 day after diagnose of sepsis.
Secondary	The plasma level of chemokine MIP-1a.	The plasma level of chemokine MIP-1a will be determined by ELISA kit.	1,3,5,7,10,14 day after diagnose of sepsis.
Secondary	The plasma level of chemokine MIP-1ß.	The plasma level of chemokine MIP-1ß will be determined by ELISA kit.	1,3,5,7,10,14 day after diagnose of sepsis.
Secondary	The phagocytic index of monocytes.	The phagocytic index of monocytes will be determined by a E.coli Phagocytosis Assay Kit.	1,3,5,7,10,14 day after diagnose of sepsis.