Effects of a Sedentary Behaviors at Work on Health in Emergency Medical Dispatchers and CODIS Operators (SECODIS)

Sedentary Behavior Clinical Trial

— SECODIS  

Official title:

Effects of a Sedentary Behaviors at Work on Health in Emergency Medical Dispatchers and CODIS Operators

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT05931406
• Other study ID #: RBHP 2022 DUTHEIL
• Secondary ID: 2022-A02730-43
• Status: Not yet recruiting
• Phase: N/A
• Start date: June 2024
• Est. completion date: September 2026

Study information

• Verified date: June 2023
• Source: University Hospital, Clermont-Ferrand
• Contact: Lise LACLAUTRE
• Phone: 334.73.754.963
• Email: promo_interne_drci[@]chu-clermontferrand.fr
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of the study is to study changes in sedentary behavior following a behavioral intervention (sit-and-stand desk, and cycloergometer)

Description:

Each emergency medical services dispatcher and each firefighter participates in the study for three days. A normal working day from of 12h is compared to: (i) a working day during which the participants have to get up at least 5 min/hour (sit-and-stand desk), (ii) a working day during which they can use a cycloergometer installed under the desk. Conditions will be random using Latin Square design with stratification between sequences cross-over design on occupation (EMD vs. firefighters).

The heart rate variability (measured using Zephyr®), the electrodermal activity (Empatica® E4), the level of physical activity (Actigraph®) as well as the blood sugar (Freestyle®) will be measured continuously during the 12 hours of work and the night after except for Actigraph® and Freestyle® device that will be let one week. Blood and saliva samples will be collected on each working day, at the beginning and end of the day i.e. two measures for blood samples, and every three hours i.e. 5 measures for saliva sampling. Participants will have to complete a detailed questionnaire to identify the particular events (vital emergencies, etc.) that may influence the parameters measured.

Participants will be asked to answer a general questionnaire once at the start of the study that will cover different aspects:

• Sociodemographic,

• Visual analogue scale (VAS) related to psychosocial factors : stress at home, burnout / burnout, decision latitude / autonomy at work, psychological demands at work (workload), support from the hierarchy, support from co-workers, family support, job satisfaction with regard to effort, commitment to work, addiction to work, quality of life, need for psychological support,

• Eating habits.

They will then have to answer a short questionnaire at the beginning and at the end of each measurement day. It will cover different aspects:

• Four VAS (stress level, fatigue, anxiety, mood),

• Physical activity and sedentary lifestyle (number of hours of physical activity and sitting during the last 24 hours),

• Daily consumption (tobacco, alcohol, cannabis, coffee, tea).

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 36
• Est. completion date: September 2026
• Est. primary completion date: December 2024
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Emergency medical dispatchers or firefighters from the departmental fire and rescue operational center (CODIS).

• Person able to give an informed consent to participate in research

• Affiliated with a Social Security scheme.

• Being able to use the sit-stand desk and the cycle ergometer

Exclusion Criteria:

• Non-affiliated to a health insurance,

• Protected persons (minors, pregnant women, breastfeeding women, guardianship, curatorship, deprived of freedoms, safeguard of justice),

• Refusal to participate.

Related Conditions & MeSH terms

• Emergencies
• Occupational Stress
• Sedentary Behavior

Intervention

Behavioral:
• Control
A normal working day from of 12h
• Sit-and-stand desk
a working day during which the participants have to get up at least 5 min/hour
• Cycloergometer
a working day during which they can use a cycloergometer installed under the desk

Locations

Country	Name	City	State
France	CHU clermont-ferrand	Clermont-Ferrand

Sponsors (1)

Lead Sponsor	Collaborator
University Hospital, Clermont-Ferrand

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes per day standing/active.	12 hours of work corresponding to the control condition
Primary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes per day standing/active.	12 hours of work corresponding to the sit-and-stand desk condition
Primary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes per day standing/active.	12 hours of work corresponding to the cycloergometer condition
Secondary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes standing/active during one week.	one-week (168 hours) sit-and-stand desk cycle
Secondary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes per day standing/active.	one-week (168 hours) control cycle
Secondary	Sedentary behavior	Sedentary behavior measured by actimetry using Actigraph® in number of minutes standing/active during one week.	one-week (168 hours) cycloergometer cycle
Secondary	Heart rate variability	The heart rate variability measured using Zephyr®	24 hours record corresponding to 12 hours of work and the next 12 hours of rest
Secondary	Electrodermal activity	The electrodermal activity measured using Empatica E4	24 hours record corresponding to 12 hours of work and the next 12 hours of rest
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the control day
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the sit-and-stand desk day
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the cycloergometer day
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the control day
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the sit-and-stand desk day
Secondary	Stress at home	Stress at home measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the cycloergometer day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the control day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the sit-and-stand desk day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the cycloergometer day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the control day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the sit-and-stand desk day
Secondary	Fatigue	Fatigue measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the cycloergometer day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the control day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the sit-and-stand desk day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 am, beginning of the cycloergometer day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the control day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the sit-and-stand desk day
Secondary	Anxiety	Anxiety measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	6 pm, end of the cycloergometer day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent). Higher scores mean a better outcome.	6 pm, beginning of the control day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent).Higher scores mean a better outcome.	6 am, beginning of the sit-and-stand desk day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent).Higher scores mean a better outcome.	6 am, beginning of the cycloergometer day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent).Higher scores mean a better outcome.	6 pm, end of the control day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent).Higher scores mean a better outcome.	6 pm, end of the sit-and-stand desk day
Secondary	Mood	Mood measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent).Higher scores mean a better outcome.	6 pm, end of the cycloergometer day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 am, beginning of the control day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 am, beginning of the sit-and-stand desk day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 am, beginning of the cycloergometer desk day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 pm, end of the control day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 pm, end of the sit-and-stand desk day
Secondary	Stress at work	Stress at work measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum).Higher scores mean a worse outcome.	6 pm, end of the cycloergometer day
Secondary	Amount of physical activity	Number of hours of physical activity during the last 24 hours	6 am, beginning of the control day
Secondary	Intensity of physical activity	Physical activity intensity during the last 24 hours measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 am, beginning of the control day
Secondary	Amount of physical activity	Number of hours of physical activity during the last 24 hours	6 am, beginning of the sit-and-stand desk day
Secondary	Intensity of physical activity	Physical activity intensity during the last 24 hours measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 am, beginning of the sit-and-stand desk day
Secondary	Amount of physical activity	Number of hours of physical activity during the last 24 hours	6 am, beginning of the cycloergometer day
Secondary	Intensity of physical activity	Physical activity intensity during the last 24 hours measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 am, beginning of the cycloergometer day
Secondary	Amount of physical activity	Number of hours of physical activity during the working day	6 pm, end of the control day
Secondary	Intensity of physical activity	Physical activity intensity during the working day measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 pm, end of the control day
Secondary	Amount of physical activity	Number of hours of physical activity during the working day	6 pm, end of the sit-and-stand desk day
Secondary	Intensity of physical activity	Physical activity intensity during the working day measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 pm, end of the sit-and-stand desk day
Secondary	Amount of physical activity	Number of hours of physical activity during the working day	6 pm, end of the cycloergometer day
Secondary	Intensity of physical activity	Physical activity intensity during the working day measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a higher intensity.	6 pm, end of the cycloergometer day
Secondary	Sitting time	Number of hours sit during the last 24 hours	6 am, beginning of the control day
Secondary	Sitting time	Number of hours sit during the last 24 hours	6 am, beginning of the sit-and-stand desk day
Secondary	Sitting time	Number of hours sit during the last 24 hours	6 am, beginning of the cycloergometer day
Secondary	Sitting time	Number of hours sit during the working day	6 pm, end of the control day
Secondary	Sitting time	Number of hours sit during the working day	6 pm, end of the sit-and-stand desk day
Secondary	Sitting time	Number of hours sit during the working day	6 pm, end of the cycloergometer day
Secondary	Tabacco consumption	Number of cigarette during the last 24h	6 am, beginning of the control day
Secondary	Tabacco consumption	Number of cigarette during the last 24h	6 am, beginning of the sit-and-stand desk day
Secondary	Tabacco consumption	Number of cigarette during the last 24h	6 am, beginning of the cycloergometer day
Secondary	Tabacco consumption	Number of cigarette during the working day	6 pm, end of the control day
Secondary	Tabacco consumption	Number of cigarette during the working day	6 pm, end of the sit-and-stand desk day
Secondary	Tabacco consumption	Number of cigarette during the working day	6 pm, end of the cycloergometer day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 am, beginning of the control day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 am, beginning of the sit-and-stand desk day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 am, beginning of the cycloergometer day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 pm, end of the control day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 pm, end of the sit-and-stand desk day
Secondary	Tea consumption	Number of cups of tea during the last 24h	6 pm, end of the cycloergometer day
Secondary	Coffee consumption	Number of cups of coffee during the last 24h	6 am, beginning of the control day
Secondary	Coffee consumption	Number of cups of coffee during the last 24h	6 am, beginning of the sit-and-stand desk day
Secondary	Coffee consumption	Number of cups of coffee during the last 24h	6 am, beginning of the cycloergometer day
Secondary	Coffee consumption	Number of cups of coffee during the working day	6 pm, end of the control day
Secondary	Coffee consumption	Number of cups of coffee during the working day	6 pm, end of the sit-and-stand desk day
Secondary	Coffee consumption	Number of cups of coffee during the working day	6 pm, end of the cycloergometer day
Secondary	Sociodemographic	Sociodemographic will be assessed: gender, age, marital status, children, occupation, and recent stressful event	Once at baseline
Secondary	Sleep quality	Sleep quality measured using visual analogue scale ranging from 0 (Bad) to 100 (Excellent). Higher scores mean a better outcome.	Once at baseline
Secondary	Family support	Family support measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a better outcome.	Once at baseline
Secondary	Burnout	Burnout measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	Once at baseline
Secondary	Job demand	Job demand measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a worse outcome.	Once at baseline
Secondary	Job control	Job control measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a better outcome.	Once at baseline
Secondary	Hierarchy support	Hierarchy support measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a better outcome.	Once at baseline
Secondary	Colleagues support	Colleagues support measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a better outcome.	Once at baseline
Secondary	Effort reward-imbalance	Effort reward-imbalance measured using visual analogue scale ranging from 0 (Minimum) to 100 (Maximum). Higher scores mean a better outcome.	Once at baseline
Secondary	Medical treatment	Medical treatment measured using multi-choice question "do you take long term medication?"	Once at baseline
Secondary	Allergy	Allergy measured using multi-choice question "do you have allergy?"	Once at baseline
Secondary	Average physical activity	Average number of hours of physical activity per week	Once at baseline
Secondary	Average physical activity intensity	Average physical activity intensity per week	Once at baseline
Secondary	Average tabacco consumption	Average number of cigarette per day	Once at baseline
Secondary	Average alcohol consumption	Average number of alcohol glasses per week	Once at baseline
Secondary	Average cannabis consumption	Average number of cannabis joint per week	Once at baseline
Secondary	Average tea consumption	Average number of cup of tea per day	Once at baseline
Secondary	Average coffee consumption	Average number of cup of coffee per day	Once at baseline
Secondary	Average food consumption	Average food consumption of 16 food groups	Once at baseline
Secondary	Diet characterization	"How do you characterize your diet" measured on a visual analogue scale ranging from 0 (Not at all balanced) to 100 (Very balanced)	Once at baseline
Secondary	RPAQ questionnaire	The Recent Physical Activity Questionnaire (RPAQ) assesses the level of physical activity and sedentary lifestyle in adults over the past four weeks. It is divided into three parts: work and study, home and leisure, and finally, stairs and travel. It makes it possible to classify individuals into several categories, one on sedentary lifestyle (low, resistant either 3-7h/day or high or >7h/day) and one on the level of physical activity (<8.3 Metabolic equivalent of task(MET).hour/week: inactive or >8.3 MET.h/wk: active).	Once at baseline
Secondary	MBI questionnaire	The Maslach Burn-out Inventory (MBI) is composed of 22 items designed to assess the three components of the burn-out syndrome: emotional exhaustion (9 items), depersonalization (5 items) and reduced personal accomplishment (8 items). The items are written in the form of statements about personal feelings or attitudes. Items are made of a 7-point scale frequency of feelings, varying from "never" to "every day". The scores for each component of the burn-out syndrome are considered separately. If desired for participant feedback, each score can be coded as low, average, or high.	Once at baseline
Secondary	JDCS questionnaire	The Job Demand-Control-Support (JDCS) questionnaire of Karasek assess job demands, job control and social support through 26 items. The questionnaire measures nine items of job demands, nine items of job control and eight items of social support. Items of JDCS are scored on a four-point Likert-type scale, ranging from 1 = strongly disagree to 4 = strongly agree. Among the 26 items, five negative statements require reverse scoring. From French data, the job strain threshold is set for a demands score higher than 20 and a control score lower than 71; the isostrain threshold is determined from a combining score of job strain and social support lower than 24. We would ask the participants to fulfil the questionnaire from memories that they keep of their work.	Once at baseline
Secondary	ERI questionnaire	The Effort-Reward Imbalance Questionnaire (ERI) is a self-administrated test assessing psychological distress and health problems that may occur when there is an imbalance between the efforts required by the work and the rewards received. We used the 46 items of the French version of the ERI model exploring efforts (six items), over commitment (eleven items), and rewards (seventeen items). Items of ERI were scored on a five-point Likert-type scale, ranging from 1 = disagree to 5 = agree and very disturbed. A ratio extrinsic efforts and rewards can assess the imbalance between these two dimensions. A ratio greater than one defines employees exposed to an imbalance between efforts and rewards.	Once at baseline
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 am, beginning of the control day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 am, beginning of the control day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 am, beginning of the control day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 am, beginning of the control day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	9 am on the control day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	9 am on the control day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	9 am on the control day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	9 am on the control day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	12 am on the control day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	12 am on the control day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	12 am on the control day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	12 am on the control day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	3 pm on the control day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	3 pm on the control day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	3 pm on the control day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	3 pm on the control day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 pm, end of the control day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 pm, end of the control day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 pm, end of the control day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 pm, end of the control day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 am, beginning of the sit-and-stand desk day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 am, beginning of the sit-and-stand desk day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 am, beginning of the sit-and-stand desk day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 am, beginning of the sit-and-stand desk day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	9 am on the sit-and-stand desk day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	9 am on the sit-and-stand desk day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	9 am on the sit-and-stand desk day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	9 am on the sit-and-stand desk day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	12 am on the sit-and-stand desk day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	12 am on the sit-and-stand desk day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	12 am on the sit-and-stand desk day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	12 am on the sit-and-stand desk day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	3 pm on the sit-and-stand desk day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	3 pm on the sit-and-stand desk day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	3 pm on the sit-and-stand desk day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	3 pm on the sit-and-stand desk day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 pm, end of the sit-and-stand desk day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 pm, end of the sit-and-stand desk day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 pm, end of the sit-and-stand desk day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 pm, end of the sit-and-stand desk day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 am, beginning of the cycloergometer day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 am, beginning of the cycloergometer day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 am, beginning of the cycloergometer day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 am, beginning of the cycloergometer day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	9 am on the cycloergometer day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	9 am on the cycloergometer day
Secondary	Salivary leptin	Salivary leptin measured in ng/dml	9 am on the cycloergometer day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	9 am on the cycloergometer day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	12 am on the cycloergometer day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	12 am on the cycloergometer day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	12 am on the cycloergometer day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	12 am on the cycloergometer day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	3 pm on the cycloergometer day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	3 pm on the cycloergometer day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	3 pm on the cycloergometer day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	3 pm on the cycloergometer day
Secondary	Salivary cortisol	Salivary cortisol measured in µg/dl	6 pm, end of the cycloergometer day
Secondary	Salivary DHEAS	Salivary DHEAS measured in pg/ml	6 pm, end of the cycloergometer day
Secondary	Salivary leptin	Salivary leptin measured in ng/ml	6 pm, end of the cycloergometer day
Secondary	Salivary ghrelin	Salivary ghrelin measured in ng/ml	6 pm, end of the cycloergometer day
Secondary	Blood cortisol	Blood cortisol measured in ng/ml or nmol/l	6 am, beginning of the cycloergometer day
Secondary	Blood cortisol	Blood cortisol measured in ng/ml or nmol/l	6 pm, end of the cycloergometer day
Secondary	Blood DHEAS	Blood DHEAS measured in µg/ml	6 am, beginning of the cycloergometer day
Secondary	Blood DHEAS	Blood DHEAS measured in µg/ml	6 pm, end of the cycloergometer day
Secondary	Blood leptin	Blood leptin measured in ng/ml	6 am, beginning of the cycloergometer day
Secondary	Blood leptin	Blood leptin measured in ng/ml	6 pm, end of the cycloergometer day
Secondary	Blood ghrelin	Blood ghrelin measured in pg/ml	6 am, beginning of the cycloergometer day
Secondary	Blood ghrelin	Blood ghrelin measured in pg/ml	6 pm, end of the cycloergometer day
Secondary	Blood BDNF	Blood brain-derived neurotrophic factor (BDNF) measured in pg/ml	6 am, beginning of the cycloergometer day
Secondary	Blood BDNF	Blood brain-derived neurotrophic factor (BDNF) measured in pg/ml	6 pm, end of the cycloergometer day
Secondary	Blood pro-inflammatory cytokines	Blood pro-inflammatory cytokines measured in pg/ml	6 am, beginning of the cycloergometer day
Secondary	Blood pro-inflammatory cytokines	Blood pro-inflammatory cytokines measured in pg/ml	6 pm, end of the cycloergometer day
Secondary	Blood NPY	Blood vascular distribution of neuropeptide Y (NPY) measured in pg/ml	6 am, beginning of the cycloergometer day
Secondary	Blood NPY	Blood vascular distribution of neuropeptide Y (NPY) measured in pg/ml	6 pm, end of the cycloergometer day