A Study to Assess the Pharmacokinetics, Safety and Tolerability of CT327 in Healthy Male Volunteers

Safety Clinical Trial

Official title:

A Multiple Dose, Placebo Controlled Study to Assess the Pharmacokinetics, Safety and Tolerability of Topically Administered CT327 in Healthy Male Volunteers

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01243502
• Other study ID #: 2114/06-CT327
• Status: Completed
• Phase: Phase 1
• First received: November 17, 2010
• Last updated: November 17, 2010
• Start date: September 2007
• Est. completion date: November 2007

Study information

• Verified date: November 2010
• Source: Creabilis SA
• Contact: n/a
• Is FDA regulated: No
• Health authority: United Kingdom: Medicines and Healthcare Products Regulatory Agency
• Study type: Interventional

Clinical Trial Summary

K-252a is a potent inhibitor of nerve growth factor (NGF) and therefore has the ability to inhibit keratinocyte proliferation. K-252a is strongly lipophilic and therefore passes freely into the cell membranes of keratinocytes and accumulates at a systemic level. In order to reduce the dermal absorption and reduce the possible long-term systemic toxicity this study will assess a PEGylated derivative of K-252a named CT327. This approach should improve the safety profile of the K-252a molecule while maintaining its activity. The primary objective is to assess the safety and tolerability of single and repeat doses of CT327 when applied topically to the skin of healthy male volunteers.

The secondary objective is to evaluate the eventual systemic absorption (pharmacokinetics; PK) of CT327 following single and repeat doses in healthy male subjects.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 16
• Est. completion date: November 2007
• Est. primary completion date: November 2007
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 64 Years

Eligibility

Inclusion Criteria:

• The subject is free from clinically significant illness or disease as determined by their medical history, physical examination, laboratory and other tests.

• The subject is a Caucasian male (Caucasian is defined as having origins in the original peoples of Europe, the Middle East, Western Russia, Afghanistan, or the white racial groups of Africa).

• The subject has a body mass index within the range 19 - 30 kg/m2 and has a weight of = 50 kg.

• The subject is capable of giving informed consent and complying with the restrictions and requirements of the protocol.

• The subject is available to complete the study.

• The subject has been registered with a UK General Practitioner (GP) for 3 months prior to the screening visit.

• A signed and dated consent form has been obtained from the subject in accordance with International Conference on Harmonisation Good Clinical Practice (ICH GCP).

Exclusion Criteria:

• As a result of the medical screening process, the PI or medical delegate considers the subject unfit for the study.

• The subject has significant scars, cuts, wounds, dermal abnormalities, tattoos or naevi in the test areas.

• The subject has a past history of contact dermatitis, psoriasis or keloid.

• The subject has any clinically significant abnormality, in the opinion of the PI, on 12-lead ECGs (including subjects with baseline QTcB > 430 ms) at screening.

• The subject has a history of drug or other allergy that contraindicates his participation.

• The subject has participated in a study with a new molecular entity within 4 months, or any other drug trial within 3 months of dosing with the investigational medicinal product (IMP) or placebo.

• The subject has donated a unit of blood (450 mL) in the 3 months prior to dosing or intends to donate in the month after the last scheduled study visit.

• The subject is currently taking a regular course of medication (including vitamins and herbal remedies).

• The subject regularly, or on average, drinks more than 21 units of alcohol per week (one unit of alcohol equals approximately 250 mL of beer, 125 mL of wine or 20 mL of spirits).

• The subject smokes more than five cigarettes (on average) per day, or has been a smoker of more than 5 cigarettes (on average) per day within the 3 months prior to screening.

• The subject has tested positive for hepatitis B surface antigen, hepatitis C antibody or HIV 1 and 2 antibodies.

• The subject has a history of drug abuse or has tested positive for drugs of abuse at pre-study screening. The subject has a positive screen for alcohol and / or drugs of abuse on admission prior to the first dose.

• The subject has consumed methylxanthine-containing food or beverages (e.g. coffee, tea, cola, chocolate, "powerdrinks", caffeine-containing cold remedies) in the 72 h prior to drug administration.

• The subject has used any prescription medication within 2 weeks or 5 half-lives (whichever is longer) of dosing.

• The subject has used non-prescription medication (e.g. aspirin, vitamins and herbal and dietary supplements) within 7 days prior to dosing, or 14 days if the medication contains grapefruit / grapefruit juice or St John's Wort.

• The subject (including those who have had a vasectomy) does not agree to use barrier contraception (condoms) when engaging in sexual activity with women of child bearing potential during the study and for 90 days after completion of the study.

Study Design

Allocation: Randomized, Endpoint Classification: Safety Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Caregiver, Investigator, Outcomes Assessor), Primary Purpose: Treatment

Related Conditions & MeSH terms

• Safety

Intervention

Drug:
• 0.01% CT327 (or placebo)
Cream (10 g) was applied at an even thickness over a test area on the back of 10 x 10 cm to give an exposure of 1000 µg/dm2.
• 0.001% CT327 (or placebo)
Cream (10 g) was applied at an even thickness over a test area on the back of 10 x 10 cm to give an exposure of 100 µg/dm2.

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Creabilis SA	LCG Bioscience

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Safety as defined by local tolerability, subject incidence of TEAEs, and clinically significant changes in lab safety tests, ECGs, vital signs and PE findings.	Each subject received a single topical dose of CT327 (or placebo) followed by a single topical dose of CT327 (or placebo) on five consecutive days with a washout period of 7 days between the two dosing periods.	2 weeks	Yes
Secondary	Pharmacokinetic	The secondary endpoint is determination of eventual CT327 plasma levels. PK blood sampling day 1: 0 h, 2 h, 4 h, 6 h, 12 h, 24 h, and 2 h post dose on days 9, 10, 11, 12 and 13.	2 weeks	Yes