Ketosis Impact on Signs & Symptoms of Schizophrenia and Bipolar disorderS

Psychosis Clinical Trial

— KISSeS  

Official title:

Pilot Study on Ketosis Impact on Signs and Symptoms of Schizophrenia and Bipolar Disorders

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT06426134
• Other study ID #: NL83836.018.23
• Status: Not yet recruiting
• Phase: N/A
• Start date: July 2024
• Est. completion date: December 2025

Study information

• Verified date: May 2024
• Source: Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)
• Contact: Karin Huizer, MD/PhD
• Phone: +31683048776
• Email: k.huizer[@]parnassiagroep.nl
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The goal of this clinical trial is to learn if a ketone drink can improve signs and symptoms of patients with a schizophrenia-spectrum disorder (SSD), or a bipolar-spectrum disorder (BD).

The main questions it aims to answer are:

Does a ketone drink improve information processing in patients with SSD/BD?

Other questions it aims to answer are:

Does a ketone drink improve cognitive functioning in patients with SSD/BD? Does a ketone drink improve metabolism and inflammation in patients with SSD/BD?

Research will compare the effects of the ketone drink with that of an isocaloric carbohydrate drink in the same patients ('cross-over').

Participants will:

1. drink a ketone drink and (after a wash-out period) an isocaloric control drink; after each drink:

• EEG to determine information-processing parameters (PPI and P300)

• cognitive tests

• visual analog scale of mood, energy levels, ability to focus

• indirect calorimetry to determine use of energy substrate

• blood draws

2. for 5 consecutive days:

• wear a continuous glucose monitor (CGM)

• wear a non-invasive passive sweat biomarker sensor (EnLiSense device)

• register a diet and nicotine diary

• saliva sampling (max. 4x/day, only on both intervention days)

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 24
• Est. completion date: December 2025
• Est. primary completion date: July 2025
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Patients with a first-episode psychosis (underlying schizophrenia-spectrum disorder), or patients with a (hypo)manic or depressive episode (underlying bipolar disorder)

• Age >= 18 years old

• Receiving standard care (including antipsychotic and mood stabilizing medication)

• Mentally competent to give informed consent:

Exclusion Criteria:

• Substance use as cause of psychosis or (hypo)mania

• Substance use (other than nicotine) in the week prior to study onset

• Intellectual disability

• Diabetes mellitus (type 1 or type 2)

• Metabolic disease impacting ketone metabolism (NB: these are rare disorders diagnosed during childhood)

• Liver disease

• Kidney disease

• Cardiovascular disease

• Pregnancy

• Breastfeeding

Related Conditions & MeSH terms

• Bipolar and Related Disorders
• Bipolar Disorder
• Depressive Episode
• Mania
• Manic Episode
• Psychosis
• Schizophrenia
• Schizophrenia and Related Disorders

Intervention

Dietary Supplement:
• (R)-3-hydroxybutyl (R)-3-hydroxybutyrate)
1x50g ingestion of pure dGK

Other:
• Maltodextrin, Fructose, Pectin, Sodium alginate, Sodium chloride
Isocaloric carbohydrate control (active control)

Locations

Country	Name	City	State
n/a

Sponsors (5)

Lead Sponsor	Collaborator
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)	EnLiSense, Parnassia Groep, The University of Texas at Dallas, University of Alberta

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Prepulse Inhibition (PPI) - change dGK vs isocaloric control	PPI: an event-related potential (ERP) representing information processing (known to be disrupted in schizophrenia and bipolar disorder).; The PPI task is an auditory paradigm featuring a total of 10 trials split evenly into two conditions: prepulse (PP) and non-prepulse (NP) in blocks. Startle pulses are 100dB at 40 ms, which is shown to provide significant startle visible in EEG126. Prepulse stimuli are 70 dB and 50 ms in duration, presented 50ms prior to the startle pulse. There is a 12 to 18 (avg: 15 s) interstimulus interval. All stimuli are white-noise blips. A calibrated apparatus is used to present the stimuli. Total estimated time is 20 min.	measured 45 minutes (Tmax) after ingestion of intervention 1 (dGK) and 45 minuts after ingestion of intervention 2 (isocaloric carb control)
Secondary	P300 Event Related Potential (change dGK vs isocaloric control)	The P300 task consists of 160 stimuli in the oddball paradigm and requires the subjects to press a button on detection of the rare target (32/160). The common nontarget stimuli are 500 Hz, 100 ms duration. The rare target stimuli are 700 Hz. Both are 100 ms duration at 80 dB with variable interstimulus interval of 1.2 to 1.5 s. Preceded by 10 practice trials. This task has minimal burden and requires about 10 minutes.	measured 65-75 minutes after ingestion of intervention 1 (dGK) and 75 minutes after ingestion of intervention 2 (isocaloric carb control); NB: directly after PPI.
Secondary	Cognitive test: 15 Word Test (15WT) - change dGK vs isocaloric control	15WT is a measure of verbal/episodic memory. The 15WT consists of 15 words, which have to be learned during three trials. After every trial the respondent is asked to recall as many words as possible. After a distraction period of 20 minutes, the respondent is asked to name the words they have learned before, again.; Immediate recall after 1 test: maximum 15 words (minimum 0) Immediate recall after 3 test: maximum 45 words (minimum 0).; Retention score after 20 minutes: percentage of correctly remembered words in the delayed recall-trial, relative to the number of words correctly remembered in the third immediate recall-trial; percentage of correctly remembered words in the delayed recall-trial, relative to the maximum score in the immediate recall-trials; Higher 15WT scores indicate better verbal/episodic memory, lower scores the opposite.	measured 75-85 minutes after ingestion of intervention 1 (dGK) and 75 minutes after ingestion of intervention 2 (isocaloric carb control); NB: directly after P300.
Secondary	Cognitive test: Trail Making Test A (TMT-A) - change dGK vs isocaloric control	TMT-A is a measure of (visual) attention. TMT-A outcome is the duration of time required to finalize test (including the time needed for the correction of errors prompted by the examiner), with a maximum of 5 minutes.; The average TMT-A score in healthy adults is 29 seconds; a deficient score is greater than 78 seconds.; Higher scores on TMT-A indicate worse (visual) attention.	measured 75-85 minutes after ingestion of intervention 1 (dGK) and 75 minutes after ingestion of intervention 2 (isocaloric carb control); NB: directly after P300.
Secondary	Cognitive test: Trail-Making Test B (TMT-B) - (change dGK vs isocaloric control)	TMT-B is a measure of (frontal) executive functioning. TMT-B outcome is the duration of time required to finalize test (including the time needed for the correction of errors prompted by the examiner), with a maximum of 5 minutes.; Average TMT-B score is 75 seconds; a deficient score is greater than 273 seconds.; Higher scores on TMT-B indicate worse (frontal) executive functioning.	measured 75-85 minutes after ingestion of intervention 1 (dGK) and 75 minutes after ingestion of intervention 2 (isocaloric carb control); NB: directly after TMT-A.
Secondary	Cognitive test: Digit Span Test (DST) (change dGK vs isocaloric control)	DST is a measure for working memory. DST outcome is the maximum number of sequential digits correctly reproduced. In average healthy adults, the digit span is around 7 +/- 2. Minimum score is 0, maximum score is 9.; Lower DST score indicate worse working memory.	measured 75-85 minutes after ingestion of intervention 1 (dGK) and 75 minutes after ingestion of intervention 2 (isocaloric carb control); NB: directly after TMT-B.
Secondary	Patient experience outcome on Mood, energy level, focus (change dGK vs isocaloric control)	visual analog scale (VAS) is used with a scale of 0 to 10 to measure patient-experiences effects of the interventions on their mood (0=extremely depression, 10 = extremely happy/euforic), energy level (0=completely exhauster, 10= extremely energetic) and ability to focus (0=completely unable to focus, 10=perfect focus).	measured circa 120 minutes after ingestion of intervention 1 (dGK) and circa 120 minutes after ingestion of intervention 2 (isocaloric carb control)
Secondary	Immune function: blood markers (change dGK vs isocaloric control)	sequential blood sampling through intravenous line: concentration (pg/ml) of inflammatory biomarkers relevant for immune function: IL-1b; IL-6; IL-8; IL-10; TNF-a; IFN-g; hsCRP; Comparison between dGK and isocaloric control at the same time points (see below).	first blood sample before ingestion (dGK or isocaloric control) (T0), then every 20 minutes in first hour after ingestion; afterwards every 30 minutes (max. 3 hours)
Secondary	Immune function: blood RNA markers (change dGK vs isocaloric control)	RNA expression analysis (immune panel nCounter, Nanostring) in whole blood: comparison dGK vs isocaloric control only at T0 plus 90 minutes.	first blood sample before ingestion (dGK or isocaloric control) (T0), next at T0+90 minutes
Secondary	Immune function: passive sweat IL-6 (change dGK vs isocaloric control)	continuous measurement of IL-6 (pg/ml) in passive sweat (EnLiSense device)	Full 5 days of the study
Secondary	Immune function: passive sweat TNF-a (change dGK vs isocaloric control)	continuous measurement of TNF-a (pg/ml)in passive sweat (EnLiSense device)	Full 5 days of study
Secondary	Metabolic function: Indirect Calorimetry (change dGK vs isocaloric control)	Resting energy expenditure (REE) is measured by gaseous exchange (indirect calorimetry). Oxygen consumption and CO2 production are measured during 20 minutes using a ventilated hood system (Q-NRG, Cosmed). Subjects lie flat on their backs and breathe into a canopy for 20 minutes. REE and Respiratory Quotient (RQ) are calculated with these measurements. The RQ represents the ratio of CO2 exhaled to the amount of 02 consumed by the individual and represents whole body substrate oxidation (glucose, fat and protein oxidation).	circa 90-120 minutes after ingestion of intervention 1 (dGK) and intervention 2 (isocaloric control); directly after finalizing cognitive tests.
Secondary	Metabolic function: blood biomarkers - change dGK vs isocaloric control	Sequential blood sampling through intravenous line, to determine the plasma and serum concentrations of: Glucose; Ketones; acylcarnitine profile; growth hormone (GH); superoxide dismutase (SOD); gluthione S-transferase (GST); soluble intercellular adhesion molecule 1 (sICAM1); Comparison between dGK and isocaloric control at the same time points (see below).	first blood sample before ingestion (both dGK and isocaloric control) (T0), then every 20 minutes in first hour after ingestion; afterwards every 30 minutes (max. 3 hours)
Secondary	Metabolic function: continuous glucose monitor (CGM) - change dGK vs isocaloric control	continuous glucose monitor (CGM; Abbott Libre Sense); continuous concentration in mM.	Full 5 days of the study.