Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Primary |
Percentage of ileal digestibility of pea proteins and casein |
Total N and 15N will be measured by Elemental Analyzer coupled to an Isotopic Ratio Mass Spectrometer (EA-IRMS) and the percentage of dietary N reaching the terminal ileum will be then determined. This percentage will be corrected by the ileal flow rate which is estimated with measurement of PEG concentration in effluents by a turbidimetric methods. Ileal digestibility of pea proteins and casein (i.e. N digestibility) will be calculated as 100 - corrected dietary N percentage. |
-30 minute to 8 hours after the meal |
|
Primary |
Percentage of ileal digestibility of amino acids from pea proteins and casein |
Amino acid concentrations will be measured by Ultra High Performance Liquid Chromatography (UHPLC) and 15N, 2H and 13C enrichment in individual amino acids will be measured by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry (GC-C-IRMS) in order to determine the percentage of dietary individual amino acids reaching the terminal ileum. This percentage will be corrected by the ileal flow rate which is estimated with measurement of PEG concentration in effluents by a turbidimetric methods. Ileal digestibility of pea and casein amino acid will be calculated as 100 - corrected dietary amino acid percentage. |
-30 minute to 8 hours after the meal |
|
Primary |
Percentage of net postprandial protein utilization of pea proteins and casein |
Urea in plasma and urine (by enzymatic methods) will be determined and associated with 15N enrichment values (by EA IRMS) to determine the 15N transfer in plasma and urinary N pools and calculate the net postprandial protein utilization of pea protein and casein. |
-30 minute to 8 hours after the meal |
|
Primary |
Postprandial concentration of dietary amino acids in plasma |
Concentration of individual amino acids (alanine, arginine, asparagine, aspartic acid, cystein, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, proline, phenylalanine, serine, threonine, valine, tryptophan, tyrosine) in plasma will be determined by UHPLC and associated with 15N enrichment values in individual amino acids (by GC C IRMS) to determined the concentration of dietary amino acids in plasma that comes from pea proteins and casein. |
-30 minute to 8 hours after the meal |
|
Primary |
Postprandial concentration of glucose in plasma |
Concentration of glucose (by enzymatic assay) will be determined in plasma. |
-30 minute to 8 hours after the meal |
|
Primary |
Postprandial concentration of insulin in plasma |
Concentration of insulin (by ELISA) will be determined in plasma. |
-30 minute to 8 hours after the meal |
|
Secondary |
Validation of the dual isotope method to assess the bioavailability of individual amino acids |
Measurements of 15N, 2H and 13C amino acids in the plasma by GC-C-IRMS. |
-30 minute to 8 hours after the meal |
|