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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT02220777
Other study ID # 8477-CL-0001
Secondary ID 2009-017865-42
Status Completed
Phase Phase 1
First received August 18, 2014
Last updated August 18, 2014
Start date November 2010
Est. completion date May 2011

Study information

Verified date August 2014
Source Astellas Pharma Inc
Contact n/a
Is FDA regulated No
Health authority France: Afssaps - Agence française de sécurité sanitaire des produits de santé (Saint-Denis)Belgium: Federal Agency for Medicines and Health Products, FAMHP
Study type Interventional

Clinical Trial Summary

This is a 4-part study. Part I assesses the safety and tolerability of single ascending doses of ASP8477 or a placebo under fasted conditions in postmenopausal subjects. Part II is similar to part I except that the study is conducted in young, vasectomized males.

Part III assesses the effect of food (fed or fasted conditions) on ASP8477 in postmenopausal subjects.

Part IV assesses the drug-drug interaction between ASP8477 and omeprazole in postmenopausal subjects.


Description:

Part I (SAD) in healthy postmenopausal females evaluates the safety, and tolerability, and defines Maximum Tolerated Dose = MTD, pharmacokinetics, and pharmacodynamics of single ascending oral doses (SAD) of ASP8477. It is anticipated that around 7 doses are required to cover the range from the safe starting dose until the MTD. Study medication is administered under fasted conditions. Based on the safety, PK, and clinical observation of the subjects, the dose escalation scheme may be adapted. Escalation to the next dose only proceeds after review of the safety, tolerability, and PK from the previous treatment period.

Part II (SAD in young, healthy, vasectomized males) is similar to Part I, one cohort of 12 subjects are treated with two doses of ASP8477 plus placebo. The doses tested depend on the safety data and pharmacodynamic profiles obtained in Part I, but is not expected to exceed MTD. The rationale for this part is to bridge the data obtained in female subjects to male subjects.

Part III (food effect) postmenopausal females receive ASP8477 once under fasted and once under fed conditions in two separate periods in random order. The dose does not exceed 1/3 of the MTD or, if that has not been reached, 1/3 of the highest tested dose in Part I. Subjects stay in the clinic for 2 periods of 5 to 7 days (depending on the terminal half life, resulting from Part I). Subjects are admitted on Day -2 per treatment period. Subjects' feeding status is the same as on Day 1 of the same treatment period. Subjects are given a single dose of ASP8477 under fed or fasting conditions on Day 1. After final discharge, subjects return for an End of Study Visit (ESV) 5-9 days later.

Part IV (ASP8477-omeprazole drug-drug interaction) postmenopausal subjects are randomized to receive omeprazole once alone and once with ASP8477 in two separate periods (in random order). The dose of ASP8477 is the MTD or, if that is not been reached, the highest tested dose in Part I. Omeprazole is chosen as target drug as it is a model substrate for CYP2C19 (accepted by FDA) and ASP8477 is known to have the strongest inhibitory effect on CYP2C19. Subjects are admitted on Day -1 and stay for 2 periods of 4 days. Subjects are given a single dose of omeprazole alone or with ASP8477 (in randomized order) on Day 1. Study medication is administered under fasted conditions. Subjects are discharged on Day 3 and return for an ESV 5-9 days later.


Recruitment information / eligibility

Status Completed
Enrollment 72
Est. completion date May 2011
Est. primary completion date May 2011
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Both
Age group 18 Years to 65 Years
Eligibility Inclusion Criteria:

- Healthy young (<65 years of age at first planned dose) postmenopausal female (Parts I, III, and IV).

- Healthy young vasectomized male subject aged 18-55 years inclusive (Part II).

- Body Mass index between 18.5 and 30.0 kg/m2 inclusive.

Exclusion Criteria:

- Known or suspected hypersensitivity to ASP8477 or any of the components of the formulations used.

- Any of the liver function tests above the upper limit of normal.

- A family history of psychiatric disorders.

- Use of grapefruit (more than 3 x 200 ml) or marmalade (more than three times) in the week prior to admission to the Clinical Unit, as reported by the subject.

- Use of xanthine-containing beverages within 48 hours before admission.

Study Design

Allocation: Randomized, Endpoint Classification: Pharmacokinetics/Dynamics Study, Intervention Model: Crossover Assignment, Masking: Double Blind (Subject, Investigator), Primary Purpose: Basic Science


Intervention

Drug:
ASP8477
oral
omeprazole
oral
Placebo
oral

Locations

Country Name City State
Belgium SGS Belgium N.V. Antwerp
France SGS Aster Paris

Sponsors (1)

Lead Sponsor Collaborator
Astellas Pharma Europe B.V.

Countries where clinical trial is conducted

Belgium,  France, 

Outcome

Type Measure Description Time frame Safety issue
Primary Safety as assessed by recording adverse events, physical examination, laboratory assessments, vital signs, electrocardiograms (ECGs), cortisol levels, arterial carbon dioxide and oxygen saturation For Part I and II Bond-Lader Visual Analogue Scale (VAS) and Bowdle VAS assessments will also be taken. Day1 to End of Study Visit (5-9 days after final discharge) No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Maximum concentration (Cmax), in plasma Maximum concentration (Cmax) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Time to attain Cmax (tmax) in plasma Time to attain Cmax (tmax) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Area Under the Curve (AUC) extrapolated until infinity (AUCinf) in plasma Area Under the Curve (AUC) extrapolated until infinity (AUCinf) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by AUC until last sample taken (AUClast) in plasma AUC until last sample taken (AUClast), Absorption lag time (tlag), Apparent terminal elimination half-life (t1/2), Apparent volume of distribution (Vz/F), Apparent total body plasma clearance (CL/F) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Absorption lag time (tlag) in plasma Absorption lag time (tlag) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Apparent terminal elimination half-life (t1/2) in plasma Apparent terminal elimination half-life (t1/2) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Apparent volume of distribution (Vz/F) in plasma Apparent volume of distribution (Vz/F) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Apparent total body plasma clearance (CL/F) in plasma Apparent total body plasma clearance (CL/F) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Amount excreted in urine until last sample (Aelast) in urine Amount excreted in urine until last sample (Aelast) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Cumulative amount of unchanged drug excreted into the urine from time zero to infinity after single dose (Aeinf) in urine Cumulative amount of unchanged drug excreted into the urine from time zero to infinity after single dose (Aeinf) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Percentage of unchanged drug excreted into the urine from time of last measurable concentration (Aelast%) in urine Percentage of unchanged drug excreted into the urine from time of last measurable concentration (Aelast%) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Percentage of unchanged drug excreted into the urine from time zero to infinity after single dose (Aeinf%) in urine Percentage of unchanged drug excreted into the urine from time zero to infinity after single dose (Aeinf%) Day 1 to Day 3 No
Primary The assessment of pharmacokinetic parameter of ASP8477 measured by Renal clearance (CLR) in urine Renal clearance (CLR) Day 1 to Day 3 No
Secondary The assessment of pharmacokinetics of omeprazole measured by plasma concentration Cmax, tmax, AUCinf, AUClast, t1/2, Vz/F, CL/F Day 1 to Day 3 No
Secondary The assessment of pharmacodynamics of ASP8477 measured by serum concentration serum concentration of endogenous substrates, volume of urine production, GFR and intra-ocular pressure Day 1 to Day 4 No