Patients in Good Health Clinical Trial
Official title:
Inflammatory Cytokines Profile in Individuals Subjected to Surgical Procedures Using Propofol or Isoflurane.
The aim of this study is to assess the profile of cytokines IL-1beta, IL-6, IL-8, IL-10, IL-12 and TNF-alpha under inhalation anesthesia with isoflurane and intravenous anesthesia with propofol in healthy patients subjected to minimally invasive elective surgeries.
Twenty ASA-I patients, subjected to otorhinolaryngology surgery, were randomly allocated in
one group to receive anesthesia with isoflurane 1 MAC (minimum alveolar concentration) (n =
20). Other group with twenty ASA-I patients received propofol 2 to 4 microgram mL-1 (n =
20).
Fentanyl 5 mg kg-1 and rocuronium bromide 0.6 mg kg-1 were also administered to all
patients. Venous blood (10 mL) was collected from each patient at each of the following
times: before the beginning of surgery and anesthesia (T1), 2 h after the beginning of
surgery (T2), and on the day after the anesthetic-surgical procedure (T3).
Plasma concentrations of interleukins IL-1beta, IL-6, IL-8, IL-10 and IL-12 and tumor
necrosis factor (TNF-alpha) were measured in each sample through flow cytometry technique by
using the method Cytometric Bead Array (CBA). Venous blood samples from fifteen volunteers
not subjected to stress were also collected as control, and the same cytokines were
measured.
;
Allocation: Randomized, Endpoint Classification: Pharmacodynamics Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Subject, Investigator), Primary Purpose: Supportive Care
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Completed |
NCT00854178 -
Toxicogenomic and Immunocytotoxic Effects of Propofol and Isoflurane Anesthetics in Patients Undergoing Elective Surgery
|
Phase 4 |