| Eligibility |
Inclusion Criteria:
1. Age = 18 and = 75, female;
2. Expected survival time > 3 months;
3. Clinically diagnosed advanced gynecological tumors, including:
1. cervical cancer;
2. Ovarian cancer;
3. Malignant trophoblastic tumor;
4. Patients who have received radical surgery ± adjuvant radiotherapy and chemotherapy,
or who have disease progression or recurrence after receiving too many lines of
radiotherapy and chemotherapy, who have been unable to be resected again or who cannot
tolerate radiotherapy and chemotherapy;
5. Patients with cervical and ovarian cancer have at least one measurable lesion
according to RECIST version 1.1 standard; Patients with malignant trophoblastic tumor
ß HCG = 5, with or without measurable lesions.
6. Voluntarily accept peripheral blood apheresis ± surgical resection of fresh tumor
tissue to obtain cells for cell preparation, and the proportion of peripheral blood
PD-1 positive T cells in the total T cells is = 18%. For patients who have received
PD-1 monoclonal antibody treatment before screening, the proportion of peripheral
blood PD1 positive T cells in the total T cells is = 10%;
7. The ECOG physical condition score is 0 to 1;
8. Have sufficient bone marrow and organ functions:
Blood system (no blood transfusion or hematopoietic stimulating factor treatment
within 14 days):
Neutrophil count (ANC) = 1.5 × one hundred and nine Platelet (PLT) = 75 × 109/L
Hemoglobin (HB) = 90g / L Lymphocyte count (lym) = 60% of the lower limit of normal
value Lymphocyte subsets: proportion of B lymphocytes (CD19 +) in lymphocytes = lower
limit of normal value
Liver function:
Total bilirubin (TBIL) = 1.5 × ULN Alanine aminotransferase (ALT) = 3 × ULN Patients
with liver metastasis or liver cancer: = 5 × ULN Aspartic acid amino transfer (AST) =
3 × ULN Patients with liver metastasis or liver cancer: = 5 × ULN
Renal function Creatinine = 1.5 × ULN Coagulation function Activated partial
thromboplastin time (APTT) = 1.5 × ULN International normalized ratio (INR) = 1.5 ×
ULN
9. Fertile women must agree to permanently use reliable contraceptive methods (hormone or
barrier method or abstinence, etc.) with their partners during and after the trial;
Women of childbearing age (as defined in Appendix 9) must have a negative blood or
urine pregnancy test within 7 days before the first use of the study drug;
10. The subjects must be informed of the study before the test and voluntarily sign a
written informed consent.
Exclusion Criteria:
1. Central nervous system metastasis or meningeal metastasis with clinical symptoms, or
there is other evidence that the patient's central nervous system metastasis or
meningeal metastasis has not been controlled, which is not suitable for inclusion
according to the judgment of the researcher;
2. Subjects with a history of second malignancy within 5 years before signing the
informed consent;
3. Patients who had previously received PD-L1 mAb;
4. Those who have active infection within 1 week before apheresis and currently need
systematic anti infection treatment;
5. Received chemotherapy, radiotherapy, biological therapy, endocrine therapy,
immunotherapy, traditional Chinese medicine with anti-tumor indications and other
anti-tumor treatments within 2 weeks before apheresis, Except for the following:
1. Nitrosourea or mitomycin C were within 6 weeks before single harvest;
2. Oral fluorouracils and small molecule targeted drugs were taken 1 week before
apheresis.
6. Within 2 weeks before apheresis:
1. Have received systemic glucocorticoid (prednisone > 10mg/day or equivalent dose
of similar drugs) or other immunosuppressant treatment; Except for the following
cases: local, eye, intra-articular, intranasal and inhaled glucocorticoids; Short
term use of glucocorticoids for preventive treatment (e.g. prevention of contrast
agent allergy);
2. Used immunomodulatory drugs, including but not limited to thymosin,
interleukin-2, interferon, etc;
7. Within 4 weeks before apheresis:
1. Have received other unlisted clinical research drugs or treatments;
2. Have undergone major organ surgery (excluding puncture biopsy) or significant
trauma, or need to undergo elective surgery during the trial;
3. Used live attenuated vaccine;
8. Currently suffering from interstitial lung disease;
9. Had received PD-1 monoclonal antibody treatment and had = grade 2 Irae; Or other
immunotherapy with = grade 3 Irae;
10. Patients with active, or had, and relapsed autoimmune diseases, such as systemic lupus
erythematosus, rheumatoid arthritis, vasculitis, etc., except for clinically stable
autoimmune thyroid disease and well controlled type I diabetes.
11. The adverse reactions of previous anti-tumor treatment have not recovered to CTCAE 5.0
and the evaluation is = 1 (except for the toxicity without safety risk judged by the
researchers such as hair loss).
12. Have a history of immune deficiency, including HIV antibody test positive;
13. Hepatitis B: the titer of HBsAg (+) and/or hepatitis B DNA is higher than that of the
research center. And/or hepatitis C: anti HCV positive; And/or Treponema pallidum
antibody positive;
14. Have a history of serious cardiovascular and cerebrovascular diseases, including but
not limited to:
1. There are serious cardiac rhythm or conduction abnormalities, such as ventricular
arrhythmia requiring clinical intervention, ?-? degree atrioventricular block,
etc.
2. Acute coronary syndrome, congestive heart failure, aortic dissection, stroke or
other grade 3 and above cardiovascular events occurred within 6 months before the
first administration.
3. New York Heart Association (NYHA) cardiac function grade = grade II or left
ventricular ejection fraction (LVEF) < 50%, or structural heart disease with high
risk judged by other researchers;
4. Clinically uncontrollable hypertension.
15. Serous effusion beyond clinical control is not suitable to be included in the group
according to the judgment of the researcher;
16. Known alcohol or drug dependence;
17. Mental disorder or poor compliance;
18. Women with reproductive needs and pregnant or lactating women;
19. There are other serious or uncontrolled systemic diseases, or other reasons that are
not suitable to participate in this clinical study.
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