Clinical Trial Details
— Status: Recruiting
Administrative data
NCT number |
NCT04382872 |
Other study ID # |
2019.137 |
Secondary ID |
|
Status |
Recruiting |
Phase |
|
First received |
|
Last updated |
|
Start date |
April 30, 2021 |
Est. completion date |
April 30, 2025 |
Study information
Verified date |
February 2023 |
Source |
Chinese University of Hong Kong |
Contact |
n/a |
Is FDA regulated |
No |
Health authority |
|
Study type |
Observational
|
Clinical Trial Summary
Folliculogenesis involves the recruitment of primordial follicles into a group of growing
follicles in which eventually one follicle is selected, matured and ovulated. Complex
endocrine and intra-ovarian paracrine interactions occur to create a changing
intra-follicular hormonal milieu suitable for oocyte development. However, this oocyte
developmental competence and to undergo fertilization and embryogenesis is impaired in aged
women. Diminished ovarian function is generally attributed to decreased quantity and quality
of oocytes and their surrounding granulosa cells during ovarian aging, although the
underlying mechanisms remain unclear.
The aim of the study is to investigate the miRNA in (follicular fluid) FF exosome in young
and aged women and their relationship to egg maturation.
Description:
In recent decades, demographic and socioeconomic factors have resulted in marked changes in
human society that include postponement of child bearing and a subsequent rise in mean age at
first childbirth. Aged women have more reproductive problems (including menstrual
irregulation and decreased ovarian reserve as reflected by reduced anti-mullerian hormone
(AMH)) and their oocytes are often of lower quality when compared to younger women.
Folliculogenesis involves the recruitment of primordial follicles into a group of growing
follicles in which eventually one follicle is selected, matured and ovulated. Complex
endocrine and intra-ovarian paracrine interactions occur to create a changing
intra-follicular hormonal milieu suitable for oocyte development. However, this oocyte
developmental competence, defined as the ability of the oocyte to complete follicular
development, and to undergo fertilization and embryogenesis is impaired in aged women.
Age-related decline in female fertility is mainly driven by ovarian aging, or diminished
ovarian function associated with age. Diminished ovarian function is generally attributed to
decreased quantity and quality of oocytes and their surrounding granulosa cells during
ovarian aging, although the underlying mechanisms remain unclear. Transcription studies have
suggested indirectly that some age-related changes in oocyte gene expression and cell to cell
communication may involve epigenetic machinery.
Ovarian follicle may be one of the best models in human for exosome study considering the
role of the granulosa cells and follicular fluid for oocyte maturation. In the ovarian
follicle, follicular fluid (FF) exosome may play an important mediated communication role
between the oocyte and surrounding granulosa cells.
The aim of the study is to investigate the miRNA in (follicular fluid) FF exosome in young
and aged women and their relationship to egg maturation.