Effect of High Omega-3 Fish Intake on Lipid Peroxidation

Omega-3 Fatty Acids Clinical Trial

Official title:

Effect of High Omega-3 Fish Intake on Lipid Peroxidation

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01183520
• Other study ID #: GFHNRC017
• Status: Completed
• Phase: N/A
• Start date: September 2010
• Est. completion date: December 2012

Study information

• Verified date: January 2022
• Source: USDA Grand Forks Human Nutrition Research Center
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The overall goal of this project is to identify an appropriate level of high omega-3 fish (salmon) consumption that will promote optimal omega 3 nutritional status without increasing the level of lipid oxidation in the body.

Description:

Studies have demonstrated that the intakes of fatty fish and fish oils are associated with decreases in cardiovascular disease and other chronic disease states. This is related to the long chain omega-3 fatty acid (n-3) content of fish and fish oil, specifically eicosapentaenoic acid and docosahexaenoic acid. Although the consumption of high n-3 fish is recommended in the 2005 US Dietary Guidelines for Americans, no specific consumption levels are made for the fatty acid eicosapentaenoic acid (EPA) and/or the fatty acid docosahexaenoic acid (DHA) or total fish intake.

Consumption of high n-3 fish or dietary supplementation of fish oil will lead to increased levels of these fatty acids in plasma lipoproteins, cell and tissue lipid. This change in membrane lipid is thought to be responsible for the anti-inflammatory effects of n-3. Because highly unsaturated fatty acid are subject to peroxidation, the level of fish intake that is sufficient to modify membrane n-3 content and the exact level that enhances peroxidation is unknown.

We will perform a dose-response feeling study in which varied levels of fish (salmon) will be provided in random order separated by 4 or more week washout periods. We will assess the level of cell membrane enrichment with n-3 and the effect on lipid peroxidation outcomes.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 30
• Est. completion date: December 2012
• Est. primary completion date: December 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 40 Years to 60 Years

Eligibility

Inclusion Criteria:

• BMI 25-34.9

• Non-smoker

• Free of major medical conditions

• Willing to comply with protocol requirements

Exclusion Criteria:

• Use of lipid modifying drugs or supplements

• Taking fish oil or flax supplements

• Regular fish consumer

• Planning to gain to lose weight

• Pregnant or lactating

Related Conditions & MeSH terms

• Lipid Peroxidation
• Omega-3 Fatty Acids

Intervention

Dietary Supplement:
• Omega-3 fish oil provided by salmon
Eating 3 different amounts of provided salmon twice a week for four weeks

Locations

Country	Name	City	State
United States	USDA ARS Grand Forks Human Nutrition Research Center	Grand Forks	North Dakota

Sponsors (3)

Lead Sponsor	Collaborator
USDA Grand Forks Human Nutrition Research Center	United States Department of Agriculture (USDA), University of North Dakota

Country where clinical trial is conducted

United States, 

References & Publications (5)

Holman RT. Control of polyunsaturated acids in tissue lipids. J Am Coll Nutr. 1986;5(2):183-211. Review. — View Citation

Raatz SK, Bibus D, Thomas W, Kris-Etherton P. Total fat intake modifies plasma fatty acid composition in humans. J Nutr. 2001 Feb;131(2):231-4. — View Citation

Roberts LJ 2nd, Fessel JP. The biochemistry of the isoprostane, neuroprostane, and isofuran pathways of lipid peroxidation. Chem Phys Lipids. 2004 Mar;128(1-2):173-86. Review. — View Citation

Roberts LJ 2nd, Montine TJ, Markesbery WR, Tapper AR, Hardy P, Chemtob S, Dettbarn WD, Morrow JD. Formation of isoprostane-like compounds (neuroprostanes) in vivo from docosahexaenoic acid. J Biol Chem. 1998 May 29;273(22):13605-12. — View Citation

Song WL, Paschos G, Fries S, Reilly MP, Yu Y, Rokach J, Chang CT, Patel P, Lawson JA, Fitzgerald GA. Novel eicosapentaenoic acid-derived F3-isoprostanes as biomarkers of lipid peroxidation. J Biol Chem. 2009 Aug 28;284(35):23636-43. doi: 10.1074/jbc.M109.024075. Epub 2009 Jun 11. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Long chain omega-3 fatty acid (n-3)	Effect of different levels of n-3 intake on n-3 fatty acids in plasma lipoproteins, cell and tissue lipids (cell enrichment)	6 months
Secondary	lipid peroxidation	Effect of different levels of n-3 intake on lipid peroxidation	6 months

External Links

•   USDA Grand Forks Human Nutrition Research Center