Nutrient Supplementation Influences Mitochondria Adaptation

Nutrition Clinical Trial

Official title:

Effect of Whey Protein Hydrolysate on Adaptions to Endurance Training in Well-trained Runners

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03561337
• Other study ID #: AURunners2017
• Status: Completed
• Phase: N/A
• Start date: October 1, 2013
• Est. completion date: June 1, 2014

Study information

• Verified date: May 2020
• Source: University of Aarhus
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Twenty-four well trained runners (VO2max 60.73.7 ml O2 Kg-1 min1) completed a six week block randomized controlled intervention period. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO- CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 0.1g carbohydrate kg-1) after each exercise session. The CHO group ingested energy matched carbohydrate. Post-absorptive muscle biopsies were obtained pre and post intervention for analysis HAD, CS and PGC-1. Subjects completed two physical tests 6 km performance test (pre, week 1 and week 6) and aVo2max test (pre and post intervention).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 24
• Est. completion date: June 1, 2014
• Est. primary completion date: February 1, 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 55 Years

Eligibility

Inclusion Criteria:

1. maximal oxygen uptake (VO2max) of >50ml O2 kg-1 min-1 for women and >55ml O2 kg-1 min-1 for men,

2. running at least 3 times a week and

3. running being the primarily training form

Exclusion Criteria:

1. use of medicine

2. diagnosed metabolic diseases

3. injuries which hindered running and body mass index (BMI) < 25 kg m-2

Related Conditions & MeSH terms

• Nutrition
• Performance
• Protein

Intervention

Dietary Supplement:
• PROTEIN-CARBOHYDRATE
PRO before and PRO-CHO after training
• CARBOHDYRATE
CHO before and after training

Locations

Country	Name	City	State
Denmark	Aarhus University, Department for Public Health, Section for Sport Science	Aarhus
Denmark	Sport Science, Department of Public Health, Aarhus University	Aarhus

Sponsors (1)

Lead Sponsor	Collaborator
University of Aarhus

Country where clinical trial is conducted

Denmark, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Maximal oxygen uptake	maximal oxygen uptake (L O2/min)	Change from week 1 to week 6
Secondary	VDAC	Mitochondria target measured by western blotting	Change from week 1 to week 6
Secondary	CytC	Mitochondria enzyme measured by western blotting	Change from week 1 to week 6
Secondary	Performance	6 km trial test	Change from week 1 to week 6
Secondary	COX-IV	Mitochondria target measured by western blotting	Change from week 1 to week 6
Secondary	HSP60	Mitochondria target measured by western blotting	Change from week 1 to week 6
Secondary	CD36	Fat transporter measured by western blotting	Change from week 1 to week 6