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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT01559428
Other study ID # MEC 09-3-088
Secondary ID TOP grant No. 91
Status Completed
Phase N/A
First received
Last updated
Start date May 2010
Est. completion date February 2, 2012

Study information

Verified date September 2018
Source Maastricht University Medical Center
Contact n/a
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

Plant sterols and stanols (also called phytosterols and phytostanols) are structurally related to cholesterol, but absorbed to a much lesser extent. Due to this structural similarity, plant sterols and stanols inhibit intestinal cholesterol absorption and lower serum LDL cholesterol concentrations by about 10% at daily intakes of 2.5 g. Plant sterol- and stanol-enriched food products are therefore widely available on the market to lower the risk for coronary heart disease. Plant sterols can undergo oxidation, which results in the formation of oxyphytosterols. Animal studies have now suggested that oxyphytosterols are atherogenic. Although oxyphytosterols have been identified in human serum samples, the effect of an increased intake of plant sterols on serum oxyphytosterol concentrations in humans is not known. On the other hand, plant stanols cannot be oxidized and lower not only cholesterol absorption, but also plant sterol absorption.

The major objective of the present study is to examine the effects of dietary plant sterols and stanols on fasting serum concentrations of oxyphytosterols. The minor objective is to investigate the effects of these products on postprandial serum oxyphytosterol concentrations.


Recruitment information / eligibility

Status Completed
Enrollment 48
Est. completion date February 2, 2012
Est. primary completion date October 26, 2010
Accepts healthy volunteers Accepts Healthy Volunteers
Gender All
Age group 18 Years to 70 Years
Eligibility Inclusion Criteria:

- Body Mass Index (BMI) between 20-30 kg/m2

- mean serum total cholesterol < 7.8 mmol/L

- mean serum triacylglycerol < 3.0 mmol/L

- mean plasma glucose < 6.1 mmol/L

Exclusion Criteria:

- unstable body weight (weight gain or loss > 3 kg in the past two months)

- active cardiovascular diseases like congestive heart failure or recent (<6 months) event (acute myocardial infarction, cerebral vascular incident)

- severe medical conditions that might interfere with the study such as epilepsy, asthma, chronic obstructive pulmonary disease, inflammatory bowel disease and rheumatoid arthritis

- indication for treatment with cholesterol-lowering drugs according to the Dutch Cholesterol Consensus

- use of medication such as corticosteroids, diuretics or lipid lowering therapy

- abuse of drug or alcohol (>21 units per week)

- not willing to stop the consumption of vitamin supplements, fish oil capsules or products rich in sterol or stanol esters 4 weeks before the start of the study (wash-in period)

- use of an investigational product within another biomedical study within the previous month

- pregnant or breast-feeding women

- not willing to give up being a blood donor (or having donated blood) from 8 weeks before the start of the study and during the study

- current smoker

- anemia. with a Hb-level below 7.5 mmol/L for men and below 7.0 mmol/L for women, as indicated by the blood bank of Maastricht

Study Design


Related Conditions & MeSH terms


Intervention

Dietary Supplement:
Plant sterol-enriched margarine
Daily consumption of 20 gram of a plant sterol-enriched margarine (providing daily 3.0 gram of plant sterols), for a period of 4 weeks. At the end of the 4 weeks, subjects will undergo a postprandial test for 8 hours, in which 20 gram of the plant sterol-enriched margarine is consumed together with a high-fat milkshake
Plant stanol-enriched margarine
Daily consumption of 20 gram of a plant stanol-enriched margarine (providing daily 3.0 gram of plant stanols), for a period of 4 weeks. At the end of the 4 weeks, subjects will undergo a postprandial test for 8 hours, in which 20 gram of the plant stanol-enriched margarine is consumed together with a high-fat milkshake
Control margarine
Daily consumption of 20 gram of a control margarine, for a period of 4 weeks. At the end of the 4 weeks, subjects will undergo a postprandial test for 8 hours, in which 20 gram of the control margarine is consumed together with a high-fat milkshake

Locations

Country Name City State
Netherlands Maastricht University Medical Centre Maastricht Limburg

Sponsors (2)

Lead Sponsor Collaborator
Maastricht University Medical Center ZonMw: The Netherlands Organisation for Health Research and Development

Country where clinical trial is conducted

Netherlands, 

Outcome

Type Measure Description Time frame Safety issue
Primary Plasma oxyphytosterol concentrations Measured at baseline and after 4 weeks. Changes will be calculated between day 28 and day 0 of each intervention period.
Secondary Serum plant sterol concentrations Measured at baseline and after 4 weeks. Changes will be calculated between day 28 and day 0 of each intervention period.
Secondary Serum lipoprotein concentrations Measured at baseline and after 3 and 4 weeks. Changes will be calculated between day 21+28 and day 0 of each intervention period.
Secondary Plasma glucose concentration Measured at day 28, on 13 time points
Secondary Markers reflecting low-grade inflammation and endothelial activation Measured at day 28, on 5 time points

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