| Eligibility |
Parts A and B Inclusion Criteria:
1. Males or females of nonchildbearing potential, of any race, 18 to 60 years of age,
inclusive, at Screening.
Females of nonchildbearing potential are defined as permanently sterile (ie, due to
hysterectomy, bilateral salpingectomy, or bilateral oophorectomy) confirmed by history
or postmenopausal (defined as at least 12 continuous months without menses and
follicle-stimulating hormone (FSH) =40 milli-International unit (mIU)/L and without an
alternative medical cause).
2. Body mass index between 18.0 and 30.0 kg/m2 , inclusive, with a minimum body weight of
50.0 kg at Screening.
3. In good health, determined by no clinically significant findings from medical history,
physical examination, 12-lead ECG, vital signs measurements, and clinical laboratory
evaluations (congenital nonhemolytic hyperbilirubinemia [eg, Gilbert's syndrome] is
not acceptable) at Screening or Check-in as assessed by the Investigator (or
designee).
4. Males will agree to use contraception.
5. Has maintained stable weight (by history) for at least 4 weeks prior to Screening.
6. Agrees to the following:
- Not to initiate a weight-loss program from Screening until the Follow-up visit;
- To refrain from strenuous exercise from 7 days before Check-in until the
Follow-up visit;
- To maintain consistent dietary habits and exercise routines for the duration of
the study.
7. Must have transaminases (aspartate aminotransferase (AST) and alanine aminotransferase
(ALT)) and total bilirubin within the normal range at Screening and Check-in. For
other laboratory evaluations, the subject may be included only if the Investigator
judges the abnormalities or deviations from normal to be not clinically significant.
8. Able to comprehend and willing to sign an informed consent form (ICF) and to abide by
the study restrictions.
Parts A and B Exclusion Criteria:
1. Significant history or clinical manifestation of any metabolic, allergic,
dermatological, hepatic, renal, hematological, pulmonary, cardiovascular,
gastrointestinal, neurological, respiratory, endocrine, or psychiatric disorder, as
determined by the Investigator (or designee).
2. Clinically significant ECG abnormalities or QT interval corrected for heart rate using
Fridericia's method (QTcF) >450 milliseconds for males and >470 milliseconds for
females at either Screening or Check-in, or any prior history of QT abnormality.
3. Creatinine clearance of <90 mL/min at Screening, determined using the Cockcroft-Gault
(C-G) equation.
4. History of febrile illness within 7 days prior to the first dose of study drug or
subjects with evidence of active infection.
5. History of significant hypersensitivity, intolerance, or allergy to any drug compound,
food, or other substance, unless approved by the Investigator (or designee).
6. History of alcoholism or drug/chemical abuse within 2 years prior to Check-in.
7. Alcohol consumption of >21 units per week for males and >14 units for females. One
unit of alcohol equals 12 oz (360 mL) beer, 1½ oz (45 mL) liquor, or 5 oz (150 mL)
wine.
8. Positive urine drug screen (confirmed by repeat) at Screening or positive alcohol
breath or urine test result or positive urine drug screen at Check-in.
9. Positive hepatitis panel and/or positive human immunodeficiency virus test. Subjects
with serologic finding of immunity consistent with history of prior hepatitis B
vaccination may be enrolled.
10. Participation in a clinical study involving administration of an investigational drug
(new chemical entity) in the past 30 days prior to dosing or have received a
biological product within 3 months or 5 elimination half-lives (whichever is longer)
prior to dosing.
11. Use of prescription drugs, nonprescription drugs, dietary supplements including
Vitamin E, herbal supplements, hormonal therapy/replacement, or CYP3A4 substrates,
inducers and inhibitors within 14 days prior to the first dose of study drug unless,
in the opinion of the Investigator and Sponsor's Medical Monitor, the medication will
not interfere with the study procedures or compromise subject safety.
12. Use or intend to use slow-release medications/products considered to still be active
within 14 days prior to Check-in, unless deemed acceptable by the Investigator (or
designee).
13. Use or intend to use any nonprescription medications/products including vitamins,
minerals, and phytotherapeutic/herbal/plant-derived preparations within 14 days prior
to the first dose of study medication, unless deemed acceptable by the Investigator
(or designee).
14. Use of tobacco- or nicotine-containing products within 3 months prior to Check-in.
15. Receipt of blood products within 2 months prior to Check-in.
16. Donation of blood from 56 days prior to Screening, plasma from 2 weeks prior to
Screening, or platelets from 6 weeks prior to Screening.
17. Poor peripheral venous access.
18. Have previously completed or withdrawn from this study or any other study
investigating CB4211, and have previously received the investigational product.
19. Subjects who, in the opinion of the Investigator (or designee), should not participate
in this study.
Part C Inclusion Criteria:
1. Males or females of nonchildbearing potential, of any race, 18 to 60 years of age,
inclusive, at Screening.
Females of nonchildbearing potential are defined as permanently sterile (ie, due to
hysterectomy, bilateral salpingectomy, or bilateral oophorectomy) confirmed by history
or postmenopausal (defined as at least 12 continuous months without menses and FSH =40
mIU/L and without an alternative medical cause).
2. Body mass index =30.0 kg/m2, and body weight =115 kg at Screening.
3. History of Fatty Liver Index (FLI) score >60, FLI score >60 at Screening, or
documented history of fatty liver with imaging results (eg, standard positive
ultrasound or Fibroscan controlled attenuation parameter (CAP) >300 decibels (dB)/m)
indicating liver fat >10% within 6 months of Screening. A formal diagnosis of
nonalcoholic fatty liver disease (NAFLD) is not required.
4. Liver fat content =10% as determined by magnetic resonance imaging derived proton
density fat fraction (MRI-PDFF) within 14 days prior to Check in. One repeat Baseline
MRI-PDFF will be allowed if the first value is below 10%, but greater than or equal to
9.5%. The repeat MRI must be within 14 days of the first MRI, and enrollment must be
within 14 days of the repeat MRI. The most current MRI will serve as the baseline.
(When available, Fibroscan indicating a CAP >300 dB/m within 6 weeks prior to Check-in
may be used as a pre-selection test prior to MRI being performed)
5. No history of common causes of secondary hepatic steatosis such as:
1. Macrovesicular steatosis: excessive alcohol consumption, hepatitis C (genotype
3), Wilson's disease, lipodystrophy, starvation, parenteral nutrition,
abetalipoproteinemia, medications (eg, amiodarone, methotrexate, tamoxifen,
corticosteroids, or any drug known to affect hepatic steatosis or insulin
resistance)
2. Microvesicular steatosis: Reye's syndrome, medications (valproate,
anti-retroviral medicines), acute fatty liver of pregnancy, HELLP syndrome,
inborn errors of metabolism (eg, lecithin-cholesterol acyltransferase deficiency,
cholesterol ester storage disease, Wolman disease, lysosomal acid lipase
deficiency)
6. Glycosylated hemoglobin A1c <7.0 % at Screening.
7. Fasting blood glucose of =100 to <126 mg/dL at Screening.
8. Serum triglyceride level =500 mg/dL at Screening
9. Have international normalized ratio (INR) < upper limit of normal (ULN) and platelet
count >150,000 at Screening and Check-in. For other abnormalities, the subject may be
included only if the Investigator judges the abnormalities or deviations from normal
to be not clinically significant.
10. In subjects with ALT, AST, alkaline phosphatase (ALP), or total bilirubin > ULN at
Screening, the baseline measurement (BLM) should be determined by 2 separate
measurements obtained approximately 4 weeks apart. To be eligible for study entry, the
Screening ALT, AST, ALP, and total bilirubin must be < ULN or the following criteria
must be met prior to randomization:
1. ALT =3 x ULN for both measurements and the difference between the measurements
should be <20% of the lower value;
2. AST =3 x ULN for both measurements and the difference between the measurements
should be <20% of the lowest value;
3. ALP =3 x ULN for both measurements and the difference between the measurements
should be <20% of the lowest value;
4. Total bilirubin =2 x ULN for both measurements and the difference between the
measurements should be <20% of the lowest value.
11. In good health, determined by no clinically significant findings from medical history,
physical examination, 12-lead ECG, vital signs measurements, and clinical laboratory
evaluations (congenital nonhemolytic hyperbilirubinemia [eg, Gilbert's syndrome] is
not acceptable) at Screening or Check-in as assessed by the Investigator (or
designee).
12. Males must agree to use contraception.
13. Has maintained stable weight (by history) for at least 4 weeks prior to Screening.
14. Agrees to maintain consistent dietary habits and exercise routines for the duration of
the study, including avoiding :
- initiating any weight-loss program from Screening until the Follow-up visit;
- strenuous exercise from 7 days before Check-in until the Follow-up visit;
15. Able to comprehend and willing to sign an ICF and to abide by the study restrictions.
Part C Exclusion Criteria:
1. Significant history or clinical manifestation of any metabolic/endocrine (except for
type 2 diabetes), allergic, dermatological, hepatic (except for NAFLD), renal,
hematological, pulmonary, cardiovascular, gastrointestinal, neurological, respiratory,
or psychiatric disorder, as determined by the Investigator (or designee).
2. Clinically significant ECG abnormalities or QTcF >450 milliseconds for males and 470
milliseconds for females at Screening, or any prior history of QT abnormality.
3. Currently using any antidiabetic medication (eg, insulin, glucagon-like peptide-1
analogs, agonist therapy) other than a stable regimen of metformin (ie, a fixed dose
of metformin for >8 weeks at Screening).
4. Use of fibrates or statins within 6 weeks or beta blocker drugs within 2 weeks prior
to Check-in and throughout the duration of the study.
5. Use of Vitamin E, agents associated with changes in liver fat, or agents used for
treatment of NAFLD or nonalcoholic steatohepatitis (NASH) within 3 months prior to
Screening and throughout the duration of the study.
6. Change in body weight >5% within the 3 months prior to Check-in.
7. History of bariatric surgery and any other gastrointestinal surgery relative to weight
loss (eg, Roux-en-Y gastric bypass, laparoscopic adjustable gastric banding, sleeve
gastrectomy, duodenal switch with biliopancreatic diversion).
8. Claustrophobia or other contraindication to magnetic resonance imaging.
9. Creatinine clearance of <90 mL/min at Screening, determined using the C-G equation.
10. History of febrile illness within 7 days prior to the first dose of study drug or
subjects with evidence of active infection.
11. History of significant hypersensitivity, intolerance, or allergy to any drug compound,
food, or other substance, unless approved by the Investigator (or designee).
12. History of alcoholism or drug/chemical abuse within 2 years prior to Check-in.
13. Alcohol consumption of >14 units per week for males and >7 units for females. One unit
of alcohol equals 12 oz (360 mL) beer, 1½ oz (45 mL) liquor, or 5 oz (150 mL) wine.
14. Positive urine drug screen (confirmed by repeat) at Screening or positive alcohol
breath or urine test result or positive urine drug screen at Check-in.
15. Positive hepatitis panel and/or positive human immunodeficiency virus test. Subjects
with serologic finding of immunity consistent with history of prior hepatitis B
vaccination may be enrolled.
16. Participation in a clinical study involving administration of an investigational drug
(new chemical entity) in the past 30 days prior to dosing or have received a
biological product within 3 months or 5 elimination half-lives (whichever is longer)
prior to dosing.
17. Use of prescription drugs, nonprescription drugs, dietary supplements including
peroxisome proliferator-activated receptor-? agonists, drugs known to affect insulin
sensitivity, herbal supplements, hormonal therapy/replacement, or CYP3A4 substrates,
inducers, and inhibitors within 14 days or 5 elimination half-lives (whichever is
longer) prior to the first dose of study drug and throughout the duration of the study
unless, in the opinion of the Investigator and Sponsor's Medical Monitor, the
medication will not interfere with the study procedures or compromise subject safety.
18. Use or intend to use slow-release medications/products considered to still be active
within 14 days prior to Check-in, unless deemed acceptable by the Investigator (or
designee).
19. Use or intend to use any nonprescription medications/products including vitamins,
minerals, and phytotherapeutic/herbal/plant-derived preparations within 14 days prior
to the first dose of study medication and throughout the duration of the study, unless
deemed acceptable by the Investigator (or designee).
20. Use of tobacco- or nicotine-containing products within 3 months prior to Check-in and
throughout the duration of the study.
21. Receipt of blood products within 2 months prior to Check-in and throughout the
duration of the study.
22. Donation of blood from 56 days prior to Screening, plasma from 2 weeks prior to
Screening, or platelets from 6 weeks prior to Screening.
23. Poor peripheral venous access.
24. Have previously completed or withdrawn from this study or any other study
investigating CB4211, and have previously received the investigational product.
25. Subjects who, in the opinion of the Investigator (or designee), should not participate
in this study.
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