Explore Intra-tumor Heterogeneity and Prognostic Value of Postoperative Blood ctDNA of ⅡA - ⅢA Stage Non-squamous NSCLC Based on the Capture-based Sequencing and UMI Technology of Burning Rock

Non-small Cell Lung Cancer Clinical Trial

Official title:

A Open Multicenter Clinical Study to Explore Intra-tumor Heterogeneity and Prognostic Value of Postoperative Blood ctDNA of ⅡA - ⅢA Stage Non-squamous NSCLC Based on the Capture-based Sequencing and UMI (Unique Molecular Identifier) Technology of Burning Rock

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03337711
• Other study ID #: A2017-001
• Status: Recruiting
• Phase: N/A
• First received: November 6, 2017
• Last updated: November 7, 2017
• Start date: May 24, 2017
• Est. completion date: May 31, 2020

Study information

• Verified date: November 2017
• Source: Sun Yat-sen University
• Contact: Hao Long, Prof
• Phone: +86 20 87343261
• Email: longhao[@]sysucc.org.cn
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The study will collect 100 ⅡA - ⅢA stage non-squamous NSCLC tumor tissue and blood specimens (Include: (1) tissue by operation: 3 specimens from different regions of each patient; (2) Blood: preoperative blood (within one week), postoperative blood (3-4 weeks after operation (before adjuvant chemotherapy), 20 weeks after operation (1 month after adjuvant chemotherapy), 1 year after operation). Capture-based sequencing(168 genes panel +UMI) will performed. All patients are recommended for "pemetrexed combined platinum" adjuvant chemotherapy for 4 cycles(Moderate-dose Chemotherapy) 3-4 weeks after operation. The clinical data should be collected until disease progression. The relationship of postoperative ctDNA and recurrence defined by clinical imaging will be analyzed, as well as the correlation of tissue and postoperative blood NGS results.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 100
• Est. completion date: May 31, 2020
• Est. primary completion date: May 31, 2020
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria:

1. Received radical surgical treatment and postoperative pathological had pointed out non-squamous NSCLC;

2. Patients with postoperative pathologic staging diagnosis of ? A - ? A period (AJCC TNM staging (version 8));

3. Female or male Age = 18 years and = 75;

4. Did not receive any of the anti-tumor treatment for current disease, such as radiation therapy, chemotherapy, or molecular target, etc;

5. Eastern Cooperative Oncology Group (ECOG) performance status of 0/1;

6. The expect survival time is more than 6 months;

7. Screening laboratory results:

(1) Hematological:Absolute Neutrophil Count (ANC) = 1500 K/mm^3 (without use of G-CSF 4 weeks prior to enrollment);Hemoglobin (Hgb) = 9 g/dL (transfusions allowed);Platelets (Plts) = 100 k/mm^3; (2) Hepatic:Bilirubin =1.5×upper limit of normal (ULN),Aspartate aminotransferase (AST) =2.5 × ULN, = 5 x ULN if liver metastases are present;Alanine aminotransferase (ALT) = 2.5 × ULN, = 5 x ULN if liver metastases are present; (3) Renal:Cr= 1.5 x ULN or Ccr= 50 mL/min, and Urine dipstick test result show urinary protein < 2+. The urinary protein = 2 + should be 24-hour urine collection and ensure 24 hours urine protein content < 1 g; 8. Patients agreed and have the ability to follow the study plan to be visited, agreed to provide blood specimens in the process of preoperative (within one week) and postoperative (3-4 weeks, 20 weeks and 1 year after surgery) and tumor tissue specimens by operation, and cooperate with other research steps.

Exclusion Criteria:

1. Second primary malignancy, other than cervical carcinoma in situ after adequate treatment, in situ malignancies or adequately treated basal cell carcinoma of the skin, localized prostate cancer after radical surgery, ductal carcinoma in situ after radical surgery;

2. Clinical evaluation for patients with inoperable;

3. A serious uncontrolled medical disorder or active infection that in the investigator's opinion would impair the participant's ability to receive study treatment;

4. Prior therapy with surgery, chemotherapy, targeted therapy and immunotherapy is not allowed;

5. Eastern Cooperative Oncology Group performance status(ECOG PS ) =2;

6. A definite clinical active infection, including but not limited to pneumonia;

7. Other situation the researchers think that doesn't fit into the group.

Related Conditions & MeSH terms

• Carcinoma, Non-Small-Cell Lung
• Non-small Cell Lung Cancer

Locations

Country	Name	City	State
China	Sun Yat-Sen university cancer center	Guangzhou	Guangdong

Sponsors (1)

Lead Sponsor	Collaborator
Sun Yat-sen University

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	recurrence or metastasis	The patient was diagnosed as recurrence or metastasis according to the computed tomography (CT) scan	two years after the last patient entered the study