Timecourse of Immobilization Experiment

Muscular Atrophy Clinical Trial

— TIME  

Official title:

Short-term Muscle Disuse Induces a Rapid and Sustained Decline in Daily Myofibrillar Protein Synthesis Rates

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02984332
• Other study ID #: 650061958
• Status: Completed
• Phase: N/A
• Start date: January 2016
• Est. completion date: October 2019

Study information

• Verified date: November 2019
• Source: University of Exeter
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The effect of unilateral lower limb immobilisation over one week on muscle atrophy

Description:

This study aims to assess the effect of one week of unilateral leg immobilization on quadriceps muscle volume and muscle protein synthesis rates. Participants wore a leg brace on one leg and ambulated on crutches during the immobilization period. Thirteen healthy young males were recruited to undertake this study.

It is currently understood that unilateral leg immobilization will result in a reduction in muscle size (Berg et al., 1996, Wall et al., 2015) and muscle protein synthesis rates after a period of disuse (de Boer et al., 2015, Glover et al., 2008). Recent research in this area has shown that even short term periods of leg immobilization (i.e. <1 week) will result in reductions in muscle size and muscle protein synthesis rates after the period of disuse (Wall et al., 2015). However we currently do not have detailed information on how muscle disuse affects muscle size and muscle protein synthesis over time during short term disuse (e.g. the first week).

The stable isotope tracer deuterium oxide (D2O) will be applied to assess cumulative muscle protein synthesis between days 0-2, 2-7 and 0-7 of the immobilization period in both the immobilized and non immobilized legs. This will be in-conjunction with bilateral vastus lateralis muscle biopsies before and after 2 and 7 days of immobilization. Furthremore, quadriceps muscle volume was assessed via MRI scans that again occured before and after 2 and 7 days of immobilization. To gain a mechanistic insight on the molecular changes during disuse a custom designed 48 well Taqman low density microarray genecard was applied to assess the transcriptional response on genes involved in muscle protein synthesis and breakdown.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 13
• Est. completion date: October 2019
• Est. primary completion date: December 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

BMI >18 and <28 no prescription medications or current illness -

Exclusion Criteria:

BMI <18 and >28 current prescription medication no history of leg injury

Related Conditions & MeSH terms

• Atrophy
• Muscular Atrophy

Intervention

Other:
• Lower limb immobilisation (leg brace)

Locations

Country	Name	City	State
United Kingdom	Sport & Health Sciences	Exeter	Devon

Sponsors (1)

Lead Sponsor	Collaborator
University of Exeter

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Muscle size	Quadriceps muscle volume measured via MRI	Days 0, 2 and 7
Secondary	Muscle protein synthesis	The stable isotope tracer deuterium oxide will be used to assess muscle protein synthesis. In conjunction with bilateral vastus lateralis muscle biopsies before and after 2 and 7 days which allow for the calculation of muscle protein synthesis (fractional synthesis rate) between days 0-2, 2-7 and 0-7.	days 0-2, 2-7 and 0-7
Secondary	Muscle strength	Skeletal muscle one repetition maximum strength was assessed using standard gym equipment	Pre and post immobilization