Muscle Damage Clinical Trial
Official title:
Recovery After Exercise-Induced Muscle Damage
Verified date | November 2023 |
Source | Norwegian School of Sport Sciences |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Interventional |
The purpose of the study is to investigate muscle stiffness in relation to muscle damaging work and to investigate how well the change in muscle stiffness correlates with the degree of muscle damage (myofibrillar disruption and necrosis). To date, the reduction in force-generating capacity is the best non-invasive marker of muscle damage. It is already established that muscle stiffness correlates well with the decline in force-generating capacity after damaging exercise. However, the correlation between degree of muscle damage and muscle stiffness has not yet been investigated. The main focus of the study is therefore to investigate the relationship between muscle stiffness and muscle damage. Further, the researchers aim to investigate how calcium cycling is affected by damaging work, and if impaired calcium cycling may partially explain the observed reduction in force-generating capacity.
Status | Completed |
Enrollment | 15 |
Est. completion date | December 20, 2020 |
Est. primary completion date | October 20, 2020 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | All |
Age group | 18 Years to 35 Years |
Eligibility | Inclusion Criteria: - 18 to 35 years of age Exclusion Criteria: - Injury to the muscle-skeletal system - Other conditions causing inability to perform heavy-load resistance exercise - Having engaged in resistance exercise targeting the m. biceps brachii once a week or more over the past year |
Country | Name | City | State |
---|---|---|---|
Norway | Norwegian School of Sport Sciences | Oslo |
Lead Sponsor | Collaborator |
---|---|
Norwegian School of Sport Sciences | Oslo University Hospital, Syddansk Universitet, Denmark, Université de Nantes, University of Copenhagen, University of Oslo |
Norway,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Other | Change in HSP70 | Localization of HSP70 in skeletal muscle using Western blotting | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Other | Change in AlphaB-crystallin | Localization of alphaB-crystallin in skeletal muscle using Western blotting | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Other | Change in Fiber-specific AlphaB-crystallin staining intensity | Change in staining intensity of AlphaB-crystallin in type-I and type-II skeletal muscle fibers using Immunohistochemistry | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Other | Change in Fiber-specific HSP70 staining intensity | Change in staining intensity of HSP70 in type-I and type-II skeletal muscle using Immunohistochemistry | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Other | Change in Fatigue | Electrical stimulation of m. biceps brachii at 20 and 50 Hz | Baseline and 1 hour after eccentric biceps curls | |
Other | Change in Muscle soreness | Subjective rating of muscle soreness using a VAS-scale (0-10) | Baseline, 15 minutes, 23 hours, 47 hours, 71 hours, and 95 hours after eccentric biceps curls | |
Other | Change in Muscle swelling (circumference) | Circumference of upper arm measured 2 cm above humeral epicondyles and midbelly of m. biceps brachii | Baseline, 15 minutes, 23 hours, 47 hours, 71 hours, and 95 hours after eccentric biceps curls | |
Other | Change in Muscle swelling (thickness) | Thickness at midbelly of m. biceps brachii using ultrasound B-mode | Baseline, 2 minutes, 23 hours, 47 hours, 71 hours, and 95 hours after eccentric biceps curls | |
Other | Change in Creatine kinase | Level of serum creatine kinase | Baseline, 2,5 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls | |
Other | Change in Myoglobin | Level of serum myoglobin | Baseline, 2,5 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls | |
Other | Change in Titin | Level of titin-N fragment in urine | Baseline, 2,5 hours, morning day 2, morning day 3, morning day 4, and morning day 5 after eccentric biceps curls | |
Other | Change in Troponin I | Level of serum Troponin I in fast and slow twitch muscle fibers | Baseline, 2,5 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls | |
Other | Change in Macrophage infiltration | Presence of macrophages in skeletal muscle using Immunohistochemistry | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Other | Muscle fiber type | Fiber type composition in cross-sections of muscle samples using Immunohistochemistry | 2 hours after eccentric biceps curls | |
Other | Muscle fiber type | Fiber type composition in cross-sections of muscle samples using Immunohistochemistry | 48 hours after eccentric biceps curls | |
Other | Muscle fiber type | Fiber type composition in cross-sections of muscle samples using Immunohistochemistry | 96 hours after eccentric biceps curls | |
Other | Change in Calcium-related protein abundances in skeletal muscle | Levels of proteins and phosphorylation status using Western blotting | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Primary | Change in muscle strength | Recovery of arm flexion torque | Baseline, and 5 minutes, 3 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls | |
Primary | Change in muscle stiffness | Muscle stiffness measured with shear wave elastography as mean young modulus in different conditions (static and dynamic) | Baseline, and 50 minutes, 3 hours, 24 hours, 48 hours, 72 hours, and 96 hours after eccentric biceps curls | |
Primary | Change in muscle damage | Development of myofibrillar disruption and necrosis observed in skeletal muscle biopsies with electron and confocal microscopy | 2 hours, 48 hours, and 96 hours after eccentric biceps curls | |
Primary | Change in calcium cycling | Calcium cycling in muscle single fibers and Sarcoplasmic reticulum-homogenate | 2 hours, 48 hours and 96 hours after eccentric biceps curls | |
Secondary | Change in organization of the tubular system in skeletal muscle | Quantification of transverse and longitudinal tubules, and number of Vacuoles in single fibers using confocal microscopy | 2 hours, 48 hours and 96 hours after eccentric biceps curls |
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