Clinical Trials Logo

Clinical Trial Summary

The purpose of this study is to investigate muscle stiffness in relation to muscle damaging work and to investigate how well the change in muscle stiffness correlates with the degree of muscle damage (myofibrillar disruption and necrosis). To date, the reduction in force-generating capacity is the best non-invasive marker of muscle damage. It is already established that muscle stiffness correlates well with the decline force-generating capacity after damaging exercise. However, the correlation between degree of muscle damage and muscle stiffness has not yet been investigated. The main focus of this study is therefore to investigate the relationship between muscle stiffness and muscle damage. Further, the researchers aim to investigate how calcium cycling is affected by damaging work, and if impaired calcium cycling may partially explain the observed reduction in force-generating capacity.

Clinical Trial Description

Regardless of whether an individual is in rehabilitation or exercise for general health or athletic performance, resistance exercise is an essential form of exercise when the goal is to increase muscle mass, strength and function. Although, resistance exercise primarily is associated with positive effects it may also result in muscle damage when the exercise is of high intensity and/or unaccustomed. This is known as exercise-induced muscle damage (EIMD) and is reflected by a substantial decrease in force-generating capacity and often accompanied by intracellular swelling and delayed onset muscle soreness. On a cellular level, EIMD include myofibrillar disruption, inflammatory response and in severe cases of EIMD; myofibre necrosis. While EIMD with its symptoms clearly is evident, its underlying mechanisms are still to be fully elaborated. One interesting hypothesis regarding the molecular basis of decreased muscle strength as a result of EIMD, is related to the strain of this exercise mode causing "popped" sarcomeres. When sarcomeres are stretched beyond actin-myosin overlap, some sarcomeres may over-stretch. This results in overload of membranes, leading to opening of stretch-activated channels, and subsequently influx of Ca2+. High levels of cytoplasmic Ca2+ may cause degradation of contractile proteins or Excitation-Contraction coupling proteins mediated through increased calpain activity. However, a recent study by Cully and colleagues (2017) suggest a protective mechanism post heavy-load strength training related to Ca2+-handling. Cully et al. observed formation of vacuoles in longitudinally connecting tubules post exercise when exposing fibers to 1.3 μM [Ca2+] in the cytoplasma. These vacuoles provide an enclosed compartment where Ca2+ can be accumulated, preventing Ca2+ from initiating damage to the muscle. The role of Ca2+-regulation in recovery of muscle function warrants further investigation and clarification. To the best of the investigators knowledge, the most valid method for estimating EIMD is by investigating myofibrillar disruption, and in some cases necrosis, in muscle biopsies. This requires many resources and is rather expensive. Currently, the best non-invasive marker of muscle damage is the force deficit observed at 48 hours post exercise. However, a measurement estimating muscle damage immediately post exercise is warranted because force deficit immediately post exercise will be confounded by muscle fatigue. A novel study performed by Lacourpaille et al. (2017) showed a strong negative correlation (-0.80) between stiffness of the muscle tissue, shear modulus, measured 30 minutes post exercise and peak isometric force measured at 48 hours post exercise and therefore a strong relationship between the decline in force production capacity and increased stiffness post exercise, suggesting a possible method to predict EIMD immediately after exercise. However, direct evidence of this association is warranted, with measurements of shear modulus and EIMD biomarkers, such as the proportion of disrupted fibers and sarcoplasmic Ca2+ regulation. The ability to predict EIMD after training is of great interest to athletes, but also patients suffering from e.g. muscular dystrophies. Being able to predict EIMD quickly and non-invasively after exercise will help employ optimal recovery. The aim of this project is to investigate the link between exercise-induced muscle damage (EIMD) as changes in shear modulus by ultrasound shear wave elastography, and muscle damage as observed in the analysis of muscle biopsies. Our hypothesis is that there is a strong relationship between muscle stiffness acute post exercise and degree of muscle damage observed in muscle biopsies. A secondary aim is to further the understanding of cellular mechanisms causing EIMD and the role of Ca2+ in the recovery of muscle function. ;

Study Design

Related Conditions & MeSH terms

NCT number NCT05036239
Study type Interventional
Source Norwegian School of Sport Sciences
Status Completed
Phase N/A
Start date December 3, 2019
Completion date December 20, 2020

See also
  Status Clinical Trial Phase
Completed NCT04136821 - The Long-term Effects of Oceanix™ on Resistance Training Adaptations N/A
Completed NCT03318731 - Efficacy and Safety of Fenugreek Extract on Markers of Muscle Damage and Inflammation in Untrained Males N/A
Recruiting NCT04986150 - Sex Differences in Muscle Damage Following Resistance Exercise With or Without Milk Protein Ingestion N/A
Not yet recruiting NCT05037942 - The Effects of Restriction Pressure on Muscle Damage Responses to Blood Flow Restriction Exercise N/A
Not yet recruiting NCT04900506 - Surgical Approach in Hemiarthroplasty. A Randomized Clinical Trial Comparing Posterior and Anterior Approach N/A
Not yet recruiting NCT05044936 - Topical Cannabidiol Cream and Post-exercise Recovery Early Phase 1
Unknown status NCT02280668 - Investigating Muscle Repair in Response to Icing Therapy Post Eccentric Muscle Damage Exercise N/A
Recruiting NCT03766815 - Effect of Branched-chain Amino Acid Supplementation on Muscle Damage N/A
Completed NCT04315077 - The Short Term Effects of Oceanix Supplementation on Recovery N/A
Completed NCT03313388 - Tart Cherry Juice for Exercise Performance and Recovery N/A
Completed NCT03753321 - Whey and Soy Protein Supplementation in Football Players N/A
Recruiting NCT03707067 - Compression Garments for Recovery in Modern Pentathletes N/A
Completed NCT03707470 - Made to Measure Compression Garments for Recovery in Rugby Players N/A
Recruiting NCT04549610 - HMB and Exercise-induced Muscle Damage Phase 2/Phase 3
Completed NCT05011643 - Exercise-induced Muscle Damage in Statin Users
Completed NCT01827696 - Effect of American Ginseng on Exercise-induced Muscle Soreness Phase 1
Completed NCT01728675 - Eccentric Exercise and Oxidative Stress N/A
Completed NCT03527797 - Diaphragm Protective Ventilation in the Intensive Care Unit N/A
Completed NCT04679519 - The Effects of Protein Supplementation in Females and Males Following Acute Eccentric Exercise N/A
Completed NCT01555775 - Compared Effect of a Fruit Milk Shake With a Protein-Carbohydrate Supplement on Recovery After Resistance Exercise N/A