Outcome
Type |
Measure |
Description |
Time frame |
Safety issue |
Primary |
Change in field activity during the speed-endurance production training (SEPT) |
Field activity will be continuously recorded during both SEPT sessions using global positioning system (GPS) technology |
Throughout the SEPT sessions in all trials |
|
Primary |
Change in heart rate during the speed-endurance production training (SEPT) |
Heart rate will be continuously recorded during both SEPT sessions using heart rate monitors. |
Throughout the SEPT sessions in all trials |
|
Primary |
Change in creatine kinase in plasma |
Concentration of creatine kinase will be measured in plasma |
Baseline; Post-loading (Day 7); 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in repeated sprint ability (RSA) |
5 x 30 m sprints will be performed with 25 seconds rest in-between |
Baseline; Post-loading (Day 7); 24 hours (Day 9) following SEPT session 1 |
|
Primary |
Change in countermovement jump (jump height in centimeters) |
Countermovement jump will be assessed on a contact platform |
Baseline; Post-loading (Day 7); 24 hours (Day 9) following SEPT session 1 |
|
Primary |
Change in isokinetic strength of lower limbs |
Isokinetic strength will be assessed on an isokinetic dynamometer for both knee extensors and knee flexors at 60 degrees. |
Baseline; Post-loading (Day 7); 24 hours (Day 9) following SEPT session 1 |
|
Primary |
Change in maximal voluntary isometric contraction (MVIC) of lower limbs |
MVIC will be assessed on an isokinetic dynamometer for knee extensors at 90 degrees and knee flexors at 30 degrees |
Baseline; Post-loading (Day 7); 1, 2 and 3 hours post-SEPT session 1; 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in delayed onset of muscle soreness |
Muscle soreness will assessed during palpation of the muscle belly and the distal region of relaxed vastus medialis, vastus lateralis and rectus femoris following three repetitions of of a full squat. Subjects will rate their DOMS on a visual analogue scale (0-10). A score of O indicates no sign or symptom of DOMS, a score of 10 indicated severe signs and symptoms of delayed onset muscle soreness (DOMS). These signs and symptoms include dull, diffuse pain and tenderness; stiffness; swelling; and decreased strength of the exercised muscle. |
Baseline; Post-loading (Day 7); 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in total antioxidant capacity in plasma |
Total antioxidant capacity will be measured in plasma |
Baseline; Post-loading (Day 7); 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in protein carbonyl levels in plasma |
Protein carbonyl levels will be measured in plasma |
Baseline; Post-loading (Day 7); 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in GSH levels in red blood cell lysate |
GSH will be measured in red blood cell lysate |
Baseline; Post-loading (Day 7); 24 hours (Day 9) and 48 hours (Day 10) following SEPT session 1. |
|
Primary |
Change in sprint time |
Sprint time will be assessed over a 10m and 30m distance using light cells. |
Baseline; Post-loading (Day 7); 24 hours (Day 9) following SEPT session 1 |
|
Secondary |
Dietary intake |
Dietary intake will be assessed using 7-day diet recalls. |
Over a 7-day period at baseline. |
|
Secondary |
Concentration of blood lactate |
Blood lactate will be measured using an automatic analyzer |
Pre- and Post-SEPT session 1 (Day 8), Pre- and Post-SEPT session 2 (Day 10) |
|
Secondary |
Change in habitual physical activity |
Physical activity will be measured using 3-axial accelerometers |
Over a 7-day period at baseline |
|
Secondary |
Change in resting metabolic rate (RMR) |
RMR will be measured using open-circuit indirect calorimeter with a ventilated hood system, after an overnight fast. |
At baseline. |
|
Secondary |
Change in body composition |
Body composition will be assessed using dual-energy X-ray absorptiometry (DXA). |
At baseline. |
|
Secondary |
Change in maximal oxygen uptake |
Maximal oxygen uptake (VO2max) will be measured during a graded exercise testing on a treadmill, by using open-circuit spirometry with an automated online pulmonary gas exchange system, via breath-by-breath analysis. |
At baseline. |
|
Secondary |
Change in soccer-specific conditioning. |
Soccer-specific conditioning will be assessed using the the Yo-Yo intermittent endurance level 2 test (Yo-Yo IE2) and the Yo-Yo intermittent recovery level 2 test (Yo-Yo IR2). |
At baseline. |
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