Examining The Effect of Blood Flow Restricted Aerobic Training on Mitochondrial Adaptations in Young Healthy Adults

Mitochondrial Biogenesis Clinical Trial

Official title:

Examining The Effect of Blood Flow Restricted Aerobic Training on Citrate Synthase Activity in Young Healthy Adults

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04151095
• Other study ID #: BFRT
• Status: Completed
• Phase: N/A
• Start date: May 6, 2019
• Est. completion date: December 13, 2019

Study information

• Verified date: December 2023
• Source: Queen's University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This parallel-arm trial study investigates the effects of restricting arterial blood flow during cycling exercise on citrate synthase. Participants will be randomized to either a blood flow restricted or non-blood flow restricted exercise training group. Both groups will complete seven, 30-minute exercise sessions over a 14-day period.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 19
• Est. completion date: December 13, 2019
• Est. primary completion date: December 13, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 30 Years

Eligibility

Inclusion Criteria:

• 3 hours of physical activity per week
• No previous cycling training
• No concurrent involvement in another training program
• Body mass index < 30 kg/m2

Exclusion Criteria:

• Presence of cardiovascular disease
• Presence of metabolic disease
• Taking regular oral medication
• Current smoker

Related Conditions & MeSH terms

• Mitochondrial Biogenesis

Intervention

Other:
• BFR Exercise Training
Participants will complete exercise training on a recumbent bike placed on a 45 degree incline (BFR).
• CTL Exercise Training
Participants will complete exercise training on a recumbent bike placed on flat ground (CTL).

Locations

Country	Name	City	State
Canada	Queen's University	Kingston	Ontario

Sponsors (1)

Lead Sponsor	Collaborator
Brendon Gurd, PhD

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Citrate Synthase	Fold change in citrate synthase maximal activity	3 days prior to start of exercise training and 3 days after completing exercise training
Secondary	Transcription factor A, mitochondrial (TFAM)	Fold change in TFAM protein	3 days before training to 3 days after training
Secondary	Nuclear respiratory factor, 1 (NRF1)	Fold change in NRF-1 protein	3 days before training to 3 days after training
Secondary	Maximal oxygen consumption (VO2peak)	Change in VO2peak (POST-PRE)	3 days before training to 3 days after training
Secondary	Heart rate (HR)	Average HR	Every 5 minutes during each of the 7, 30-minute exercise sessions
Secondary	Revolutions per minute (RPM)	Average RPM	Every 5 minutes during each of the 7, 30-minute exercise sessions
Secondary	Blood [lactate]	Average blood lactate concentration	During all exercise sessions at 10 and 30 minutes
Secondary	Rating of perceived exertion (RPE)	Average RPE	Every 5 minutes during each of the 7, 30-minute exercise sessions
Secondary	Nuclear Factor, Erythroid 2 Like 2 (NFE2L2)	Fold change in NFE2L2	3 days before training to 3 days after training
Secondary	Cytochrome c oxidase (COX)	Fold change in COX	3 days before training to 3 days after training
Secondary	Electromyography (EMG)	Median frequency and average amplitude of EMG signal	Throughout each of the 7, 30-minute exercise sessions
Secondary	Near-infrared spectroscopy (NIRS)	Deoxygenated hemoglobin and oxygenated hemoglobin	Throughout each of the 7, 30-minute exercise sessions