Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle

Mitochondrial Biogenesis Clinical Trial

Official title:

Impact of Exercise Intensity on the Time-course of Mitochondrial Biogenic Gene and Protein Expression in Human Skeletal Muscle.

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03975777
• Other study ID #: QMPL-TC-19
• Status: Recruiting
• Phase: N/A
• Start date: June 1, 2019
• Est. completion date: April 30, 2020

Study information

• Verified date: March 2020
• Source: Queen's University
• Contact: Hashim Islam, MSc
• Phone: 4037954125
• Email: 16mhi[@]queensu.ca
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 10
• Est. completion date: April 30, 2020
• Est. primary completion date: April 30, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 30 Years

Eligibility

Inclusion Criteria:

• recreationally active (no more than three hours of structured weekly physical activity)

• not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills

• body mass index < 30 kg/m2

Exclusion Criteria:

• presence of cardiometabolic disease

• taking regular prescription medication for the treatment of cardiometabolic disease

• current cigarette smoker

Related Conditions & MeSH terms

• Mitochondrial Biogenesis

Intervention

Other:
• LO
Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at ~73% of peak aerobic work rate interspersed with 1 min recovery periods)
• HI
Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at ~100% of peak aerobic work rate interspersed with 1 min recovery periods)

Locations

Country	Name	City	State
Canada	School of Kinesiology and Health Studies	Kingston	Ontario

Sponsors (1)

Lead Sponsor	Collaborator
Brendon Gurd, PhD

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1a) messenger ribonucleic acid (mRNA) expression	PGC-1a gene expression (indirect indicator of PGC-1a activity). Transcriptional co-activator involved in the control of mitochondrial biogenesis.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in PGC-1a protein content	PGC-1a protein content	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression	Nrf2 gene expression (indirect indicator of Nrf2 activity). Transcription factor involved in the control of mitochondrial biogenesis.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in Nrf2 protein content	Nrf2 protein content	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in PPAR-beta mRNA expression	PPAR-beta gene expression (indirect indicator of PPAR-beta activity). Transcription factor involved in the control of mitochondrial biogenesis.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in estrogen related receptor (ERR)-gamma mRNA expression	ERR-gamma gene expression. Transcription factor involved in the control of mitochondrial biogenesis.	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in leucine-rich pentatricopeptide repeat containing protein 130 (LRP130) mRNA expression	LRP130 gene expression. Transcriptional co-activator and RNA-binding protein involved in the transcriptional control and stabilization of mitochondrial mRNAs	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in LRP130 protein content	LRP130 protein content	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in nuclear respiratory factor-1 (NRF-1) mRNA expression	NRF-1 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in NRF-1 protein content	NRF-1 protein content	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in mitochondrial transcription factor A (TFAM) mRNA expression	TFAM gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in TFAM protein content	TFAM protein content	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in p53 mRNA expression	p53 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in cytochrome c oxidase subunit 4 (COXIV) mRNA expression.	COXIV gene expression. Nuclear encoded subunit of the electron transport chain complex 4 (COX)	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in COXI mRNA expression	COXI gene expression. Mitochondrial encoded subunit of the electron transport chain complex IV (COX)	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in COXII mRNA expression	COXII gene expression.Mitochondrial encoded subunit of the electron transport chain complex IV (COX).	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in nicotinamide adenine dinucleotide (NADH) subunit 1 (ND1) mRNA expression	ND1 gene expression. Mitochondrial encoded subunit of the electron transport chain complex 1 (NADH dehydrogenase)	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in ND4 mRNA expression	ND4 gene expression. Mitochondrial encoded subunit of the electron transport chain complex 1 (NADH dehydrogenase).	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in citrate synthase (CS) mRNA expression	CS gene expression. The first rate limiting enzyme of the Kreb's cycle.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in NRF-2 mRNA expression	NRF-2 gene expression. Transcription factor involved in the control of mitochondrial biogenesis.	before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in mitochondrial transcription factor B1 (TFB1M) mRNA expression	TFB1M gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.	before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary	Change in mitochondrial transcription factor B2 (TFB2M) mRNA expression	TFB2M gene expression. Transcription factor controlling the expression of mitochondrial-encoded genes.	before exercise and 1, 3, 6, 9, and 12 hours post-exercise