Metabolism in Adipose Tissue Clinical Trial
Official title:
Metabolism in Adipose Tissue Followed Following Sprint Exercise
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.
PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small
total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to
increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of
sprint exercise on adipose tissue metabolism the investigators examined acute short-term
effects on adipose tissue IL-6 exchange.
METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20
min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after
the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery
and a superficial subcutaneous vein on the anterior abdominal wall providing access to the
venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and
lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min
and 120 min after the last sprint. Blood flow will be determined by administration of
Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be
registrated continuously during the total investigation.
SUPPORT: This study is supported by grants from the Swedish National Center for Research in
Sports
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