Metabolic Disease Clinical Trial
— FLOWOfficial title:
Adipose Tissue Cellular Response to Exercise in Healthy Humans
Verified date | March 2022 |
Source | University of Michigan |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Interventional |
The purpose of this study is to measure the effects of a single session of exercise on changes in the cellular composition of the body fat of humans.
Status | Completed |
Enrollment | 40 |
Est. completion date | August 8, 2019 |
Est. primary completion date | August 8, 2019 |
Accepts healthy volunteers | Accepts Healthy Volunteers |
Gender | All |
Age group | 18 Years to 40 Years |
Eligibility | Inclusion Criteria: - Age: 18-40 - Body Mass Index: 20-27 kg/m2 - Regular exercise (less than or equal to 3 days/week of aerobic exercise; 30-60 minutes/session at moderate and vigorous intensities) - Women must have regularly occurring menses and must be premenopausal Exclusion Criteria: - Blood pressure greater than 140/90 mm Hg - Pregnant or lactating - Evidence/history of cardiovascular or metabolic disease - Medications known to affect lipid or glucose metabolism, or inflammation |
Country | Name | City | State |
---|---|---|---|
United States | University of Michigan | Ann Arbor | Michigan |
Lead Sponsor | Collaborator |
---|---|
University of Michigan |
United States,
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Quantifying the change in markers of macrophage abundance in adipose tissue | The stromovascular fraction will be isolated from subcutaneous lipo-aspirate samples by collagenase digestion and a series of filtration/wash steps (routinely performed by our lab). This isolation protocol has been optimized to yield the minimum number of stromal cells required to identify and recover the following cell populations by fluorescence-activated cell sorting (FACS), listed as cell surface stain = cell type: CD64+/CD11c+ = macrophages
FACS will be performed at the University of Michigan Flow Cytometry Core, and sorted cells will be recovered for subsequent gene expression analysis using the StepOnePlus real time PCR system (Life Technologies) with fluorescent probe-based assays. |
1 day | |
Secondary | Quantifying the change in markers of endothelial cell and pre-adipocytes in adipose tissue | The stromovascular fraction will be isolated from subcutaneous lipo-aspirate samples by collagenase digestion and a series of filtration/wash steps (routinely performed by our lab). This isolation protocol has been optimized to yield the minimum number of stromal cells required to identify and recover the following cell populations by fluorescence-activated cell sorting (FACS), listed as cell surface stain = cell type: CD34+/CD31+ = endothelial cells
FACS will be performed at the University of Michigan Flow Cytometry Core, and sorted cells will be recovered for subsequent gene expression analysis using the StepOnePlus real time PCR system (Life Technologies) with fluorescent probe-based assays. |
1 day |
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