NRP vs DHOPE vs COR-NMP in ECD-DCD Donation

Liver Transplant Clinical Trial

Official title:

Direct Comparison of NRP With DHOPE and COR-NMP to Maximize the Use of ECD After DCD Donation in the Netherlands

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05327478
• Other study ID #: EMC-NRP2-2022
• Status: Recruiting
• Start date: January 1, 2022
• Est. completion date: May 1, 2029

Study information

• Verified date: April 2022
• Source: Erasmus Medical Center
• Contact: Jeroen de Jonge, Dr.
• Phone: +31643904182
• Email: j.dejonge.1[@]erasmusmc.nl
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

There is still a discrepancy between the number of liver transplant candidates and the availability of liver grafts, resulting in waiting list mortality. To increase the supply of suitable liver grafts, extended-donor criteria allografts can be used. However, in the case of donation after cardiac death this is not without a risk. Donor after cardiac death (DCD) grafts have increased risk of primary non function and biliary complications, resulting in either retransplantation, patient morbidity or patient death. Due to uncertainty of their quality DCD grafts can be discarded. However, normothermic machine perfusion (NRP) has the potential to overcome these disadvantages of DCD liver grafts. In DCD livers the physiological abdominal circulation is simulated with in vivo, normothermic, oxygenated perfusion during the first two hours after cardiac death. With this perfusion technique, early ischemia can be reversed, surgical damage due to a hasty procedure can be prevented and organs can be tested on viability. In many countries, NRP is obligatory, however this is not the current golden standard in the Netherlands. The primary objective of this study is the utilization of livers after NRP. Secondary study parameters are reasons for graft discard or rejection at proposal, patient- and graft survival, biliary complications, cost assessment of NRP and outcomes of kidney and pancreas transplants. This multicenter, observational study will be performed on adult liver transplant recipients who have been allocated a DCD liver graft (Maastricht type III and V) of a donor above fifty years old. According to current national procurement protocol, grafts procured in region west will be retrieved with NRP followed by dual hypothermic oxygenated perfusion (DHOPE). Grafts retrieved in region East/North will be retrieved using standard rapid retrieval followed by DHOPE, if the donor is aged 50-60. Grafts from donors aged above 60 will undergo controlled oxygenated rewarming normothermic machine perfusion (COR-NMP) after DHOPE.

Description:

The persistent discrepancy between the number of patients awaiting liver transplant and the availability of liver grafts. In the Netherlands, still, 1 in 5 either dies on the waiting list or is removed from the waiting list due to deterioration of the disease. To increase the supply of suitable liver grafts, extended-donor criteria allografts can be used. Extended criteria donors (ECD) include older donors, donors with fatty livers, donors with high transaminase levels and donor after circulatory death (DCD). However, because of an inevitable longer period of warm ischemia, DCD livers have increased risk of primary non function and biliary complications, resulting in either patient morbidity, re-transplantation or patient death. Due to uncertainty of the effect of the longer ischemia time on their quality, DCD grafts can be accepted with reluctance or even discarded.

The persistent organ shortage has forced transplant programs to explore options to expand the organ supply. Parallel to this, interest in use of extended criteria donors (ECD) livers drove an evolution in current technique to retrieve, preserve and improve livers before transplantation. Before the era of perfusion, the organs were retrieved by using standard rapid retrieval (SRR) and preserved by static cold storage (SCS). In 1930 researchers have explored machine perfusion as a technique to preserve organs, but nowadays this technique has many more potentials including testing and improving. Currently, there is a wide diversity in different perfusion techniques, varying from in vivo to ex vivo and hypothermic (8-12°C) to normothermic (35-37°C). The rationale behind perfusion is to restore the flow through the organs with a pump to provide oxygen and drugs.

In normothermic regional perfusion (NRP) the physiological abdominal circulation is simulated with in vivo, normothermic, oxygenated perfusion during the first two hours after circulatory death. NRP replaces SRR, because this techniques allows a surgeon to retrieve the organs in a longer timeframe during this perfusion. Also, NRP can reverse early ischemia, reduce ischemic type biliary lesions, prevent surgical damage due to a more relax operation and the potential to test organs. The possibility to test the organ during NRP enables a save environment to transplant livers from older DCD donors above 60 years old. , In many countries, NRP is obligatory in DCD donation, however this is not the current golden standard in the Netherlands. Only in the Dutch Western region the livers of donors after circulatory death (Maastricht type III and V) above 50 years old will undergo NRP.

Independently of age or region, all DCD livers in the Netherlands will undergo dual hypothermic oxygenated perfusion (DHOPE) in the receiving hospital. DHOPE is a dual (through both portal vein and hepatic artery) hypothermic, ex vivo, machine perfusion technique, which depends on the presence of sufficient oxidative mitochondrial metabolism.

subsequentially, oxygenated mitochondria metabolize due to ischemia accumulated succinate and recharge adenosine tri-phosphate, which leads to less reactive oxygen species, less danger signaling and to reduced overall downstream inflammation during later normothermic reperfusion at implantation. , In comparison with SCS, DHOPE has a significant reduction in postreperfusion syndrome, early allograft dysfunction, and non-anastomotic strictures after DCD. For this reason, this perfusion technique is incorporated in the Dutch national protocol for all DCD liver grafts and financed by personal health insurances. High risk donor livers or livers from donors above sixty years old in the Northeastern region will additionally undergo controlled oxygenated rewarming normothermic machine perfusion (COR-NMP) after DHOPE. Normothermic ex vivo machine perfusion has the opportunity to test ECD grafts before transplant. The controlled oxygenated rewarming prevents sudden temperature shifts and subsequentially reduces ischemia reperfusion injury.

The above mentioned techniques with either cold or warm perfusion serve two different goals:

cold perfusion (DHOPE) prevents cholangiopathy and warm perfusion (NMP/NRP) can provide a testing area for the organ. Considering two promising warm perfusion techniques, in 2021 the new national protocol was written were NRP was performed in the west and NMP in the northeastern region. To distinguish the differences between these techniques, all the outcomes should be registered. The question remains if NRP and COR-NMP are comparable to each other in patient outcome graft- and patient survival. If these technique are equivalent in outcomes, this techniques can be distinguished based on other characteristics such as utility, cost effectiveness and influence on other organs. The definition of utility is very broad, but in this registration utilization is defined as the offer of a liver from a transplant coordinator to a successful transplant. One other characteristic to distinguish between the different forms of perfusion is cost-effectiveness. NRP may be more cost-effective than NMP since multiple organs are perfused in one procedure, which could potentially also be beneficial for the other perfused organs. As NRP also perfuses other organs, the question remains how this organs respond on this perfusion technique. For kidneys there is evidence that graft survival is similar after donation after brain death (DBD), NRP and SRR.4 The pancreas will not be transplanted after NRP, but in some cases the pancreas can be used for island isolation.

In the context of a wide variety of different perfusion techniques in one small country, the data of these different perfusion techniques are urgently needed to improve procurement, graft outcome and preservation strategies.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 150
• Est. completion date: May 1, 2029
• Est. primary completion date: May 1, 2024
• Accepts healthy volunteers: No
• Gender: All
• Age group: 50 Years to 75 Years

Eligibility

Inclusion criteria:

• DCD donor (Maastricht type III and V)

• Age above 50 years old and below 75 years old

Exclusion criteria:

• Malignancy (except for primary non-metastatic central nervous system tumors, non-melanoma skin tumors or cured malignancies)

• Active infection (sepsis, meningitis, human immune deficiency virus, rubella, rabies, herpes zoster, tuberculosis)

• Intravenous drug abuse

• Unknown cause of death

• In the case of a NRP procedure: donors with a BMI above 35 and transaminases above 1000 U/I and not decreasing prior to donation are excluded

Related Conditions & MeSH terms

• Complication of Surgical Procedure
• Liver Transplant

Intervention

Procedure:
• NRP
Normothermic Regional Perfusion
• DHOPE
Dual Hypothermic Oxygenated Perfusion
• COR-NMP
Controlled Oxygenated Rewarming Normothermic Machine Perfusion

Locations

Country	Name	City	State
Netherlands	Erasmus MC	Rotterdam	Zuid-Holland

Sponsors (3)

Lead Sponsor	Collaborator
Erasmus Medical Center	Leiden University Medical Center, University Medical Center Groningen

Country where clinical trial is conducted

Netherlands, 

References & Publications (3)

de Vries Y, Matton APM, Nijsten MWN, Werner MJM, van den Berg AP, de Boer MT, Buis CI, Fujiyoshi M, de Kleine RHJ, van Leeuwen OB, Meyer P, van den Heuvel MC, de Meijer VE, Porte RJ. Pretransplant sequential hypo- and normothermic machine perfusion of suboptimal livers donated after circulatory death using a hemoglobin-based oxygen carrier perfusion solution. Am J Transplant. 2019 Apr;19(4):1202-1211. doi: 10.1111/ajt.15228. Epub 2019 Jan 23. — View Citation

van de Leemkolk FEM, Schurink IJ, Dekkers OM, Oniscu GC, Alwayn IPJ, Ploeg RJ, de Jonge J, Huurman VAL. Abdominal Normothermic Regional Perfusion in Donation After Circulatory Death: A Systematic Review and Critical Appraisal. Transplantation. 2020 Sep;104(9):1776-1791. doi: 10.1097/TP.0000000000003345. — View Citation

van Rijn R, Schurink IJ, de Vries Y, van den Berg AP, Cortes Cerisuelo M, Darwish Murad S, Erdmann JI, Gilbo N, de Haas RJ, Heaton N, van Hoek B, Huurman VAL, Jochmans I, van Leeuwen OB, de Meijer VE, Monbaliu D, Polak WG, Slangen JJG, Troisi RI, Vanlander A, de Jonge J, Porte RJ; DHOPE-DCD Trial Investigators. Hypothermic Machine Perfusion in Liver Transplantation - A Randomized Trial. N Engl J Med. 2021 Apr 15;384(15):1391-1401. doi: 10.1056/NEJMoa2031532. Epub 2021 Feb 24. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Organ utilization rate in percentage	Number of transplanted organs divided by number offered organs	01-01-2022 to 01-05-2023
Primary	Survival in days	Patient and graft survival	1 year post transplantation
Primary	Cost effectiveness in difference between treatments in euro's	All medical costs resulting from treatment	One year post transplantation
Secondary	Cumulative complications summarized by the comprehensive complication index (CCI)	Complications will be ranked by the CCI	One year post transplantation
Secondary	Number of patients with Ischemic cholangiopathy (IC) and anastomotic strictures (AS)	IC is also referred to as non-anastomotic strictures (NAS) or ischemic type biliary lesions (ITBL). IC is defined as a symptomatic patient (e.g., jaundice or cholangitis) with radiological proven irregularity or lumen narrowing in the intra- or extrahepatic donor bile duct in absence of hepatic artery thrombosis. Isolated strictures at the anastomosis are anastomotic strictures.	One year post transplantation
Secondary	Length of stay	Length of stay in the hospital during the peritransplant admission, measured in days after the transplantation excluding readmission. Also, length of stay on the intensive care unit during the peritransplant admission, including readmission.	During peritransplant admission, up to discharge.
Secondary	Number of patients with Early allograft dysfunction (EAD)	EAD is defined as patient or graft death before the post-operative day (POD) 90	Post operative day 90
Secondary	Waiting list time in days	Per center	01-01-2022 to 01-05-2023
Secondary	Waitlist mortality in percentage	Per center	01-01-2022 to 01-05-2023