Remote Ischemic Postconditioning Increases HIF-1α Plasma Levels and Improves Cardiac Markers After Cardiac Surgery

Ischemia-reperfusion Injury Clinical Trial

Official title:

Remote Ischemic Postconditioning Increases HIF-1α Plasma Levels and Improves Cardiac Markers After Cardiac Surgery: a Randomised Controlled Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04284592
• Other study ID #: PI07/0836
• Status: Completed
• Phase: N/A
• Start date: October 2012
• Est. completion date: October 2017

Study information

• Verified date: February 2020
• Source: INCLIVA
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Background. Cardiopulmonary bypass in on-pump cardiac surgery (OPCS) can have harmful effects by ischemia-reperfusion. No data about the effects of remote ischemic postconditioning (RIP) in hypoxia-inducible factor-1 alpha (HIF-1α) plasma level after OPCS. The aim of this study is evaluate the effects of RIP on postoperative HIF-1α plasma levels, cardiac markers and arterial oxygenation of patients after OPCS.

Methods. Randomised controlled study in 70 patients undergoing OPCS: 35 patients receive RIP (RIP group) and 35 patients not (control group). Patients receive RIP on upper limb: 5 min of ischemia followed by 5 min of reperfusion (3 cycles) immediately after leaving on-pump. The primary outcome was to know the HIF-1α plasma levels after surgery in both groups: before starting surgery (T0) and after CPB period at 2 h (T1), 8 h (T2), 24 h (T3), 36 h (T4), 48 h (T5). Secondary outcomes included to measure the cardiac markers levels (Troponin T, CK-MB, CPK), arterial oxygenation (PaO2/FiO2) and others.

Description:

Cardiac surgery with cardiopulmonary bypass (CPB) is associated with ischemia-reperfusion (I-R) injury. Acute myocardial injury after cardiac surgery is associated with increased mortality and morbidity. In 1993, reported that brief circumflex artery occlusion reduces myocardial infarct size induced by a definitive occlusion of the left anterior descending artery, a phenomenon which has been named remote ischemic preconditioning (RIPC). This approach confers resistance to subsequent ischemic episodes in remote organs, possibly by transferring protective mediators through humoral, neuronal, and systemic mechanisms, however the mechanisms involved are not yet fully known. A recent study has found that cardioprotection induced by RIPC the mitochondria appear to be an important subcellular effector organelle. It has been reported that RIPC could be a potential protective approach for perioperative complications. Therefore, the effects on myocardial injury and clinical outcome in patients undergoing on-pump cardiac surgery (OPCS) are inconclusive. In the present study, we conducted a randomized clinical trial on patients undergoing OPCS by application of remote ischemic postconditioning (RIP), an alternative to remote ischemic preconditioning. RIP can be applied in different clinical situations, medical or surgical, when reperfusion is initiated and associated to acidosis, nitric oxide formation, mitochondrial permeability transition pore inhibition and reactive oxygen species generation. In the present study we have opted for remote postconditioning because we speculate a possible losses of plasmatic effectors during CPB procedures using polyvinylchloride (PVC) tubing. Since, when blood is exposed to the PVC surface the plasma proteins may be adsorbed. Also to minimize the bleeding or hemodilution effects on the potential protective plasmatic effectors levels during CPB.

Tissue exposure to low O2 concentration starts a hypoxic response of the hypoxia-inducible factor 1 (HIF-1), a transcription factor heterodimer, consisting of an O2 regulated HIF-1α subunit and a constitutively expressed HIF-1β subunit that binds to the consensus sequence 5'-RCGTG-3', which is present near HIF-1-regulated genes. HIF-1α protein stability is upregulated in response to hypoxia. HIF-1α protein stability is negatively regulated by O2-dependent prolyl hydroxylation and is degraded under normoxia by prolyl hydroxylase. HIF-1α plays a critical role in the mammalian cells activating genes associate with angiogenesis, ischemia, energy metabolism and cell cycle.

The aim of this study carried out in patients undergoing OPCS was the determination of the time course of HIF-1α plasma levels in response to RIP and the possible correlation between aortic cross-clamping time and HIF-1α plasma levels in both groups. HIF-1α role in remote ischemic conditioning (pre or post) is very little known. Therefore, we tested the hypothesis that RIP induces cardiac protection after OPCS and that this is associated to an increase of HIF-1α plasma levels.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 70
• Est. completion date: October 2017
• Est. primary completion date: June 2015
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 80 Years

Eligibility

Inclusion Criteria:

• elective cardiac surgery (coronary arteries and/or valve replacement)

• ASA physical status III or less

Exclusion Criteria:

• pregnancy

• previous cardiac surgery

• myocardial infarction (< 6 weeks)

• renal failure

• severe chronic pulmonary disease.

Related Conditions & MeSH terms

• Ischemia
• Ischemia-reperfusion Injury
• Reperfusion Injury

Intervention

Procedure:
• Remote ischemic post-conditioning (RIP)
The limb RIP was applied after leaving of cardiopulmonary bypass and consisted of 3 cycles: 5 min of ischemia by a cuff-inflator on an arm and inflated to 200 mmHg, followed by 5 min deflated. The control group had during the same time a deflated cuff.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
José García de la Asunción

References & Publications (8)

Cai Z, Luo W, Zhan H, Semenza GL. Hypoxia-inducible factor 1 is required for remote ischemic preconditioning of the heart. Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17462-7. doi: 10.1073/pnas.1317158110. Epub 2013 Oct 7. — View Citation

García-de-la-Asunción J, Pastor E, Perez-Griera J, Belda FJ, Moreno T, García-del-Olmo E, Martí F. Oxidative stress injury after on-pump cardiac surgery: effects of aortic cross clamp time and type of surgery. Redox Rep. 2013;18(5):193-9. doi: 10.1179/1351000213Y.0000000060. — View Citation

Hausenloy DJ, Candilio L, Evans R, Ariti C, Jenkins DP, Kolvekar S, Knight R, Kunst G, Laing C, Nicholas J, Pepper J, Robertson S, Xenou M, Clayton T, Yellon DM; ERICCA Trial Investigators. Remote Ischemic Preconditioning and Outcomes of Cardiac Surgery. N Engl J Med. 2015 Oct 8;373(15):1408-17. doi: 10.1056/NEJMoa1413534. Epub 2015 Oct 5. — View Citation

Hausenloy DJ, Yellon DM. Ischaemic conditioning and reperfusion injury. Nat Rev Cardiol. 2016 Apr;13(4):193-209. doi: 10.1038/nrcardio.2016.5. Epub 2016 Feb 4. Review. — View Citation

Heusch G, Bøtker HE, Przyklenk K, Redington A, Yellon D. Remote ischemic conditioning. J Am Coll Cardiol. 2015 Jan 20;65(2):177-95. doi: 10.1016/j.jacc.2014.10.031. Review. — View Citation

Kalakech H, Tamareille S, Pons S, Godin-Ribuot D, Carmeliet P, Furber A, Martin V, Berdeaux A, Ghaleh B, Prunier F. Role of hypoxia inducible factor-1a in remote limb ischemic preconditioning. J Mol Cell Cardiol. 2013 Dec;65:98-104. doi: 10.1016/j.yjmcc.2013.10.001. Epub 2013 Oct 17. — View Citation

Przyklenk K, Bauer B, Ovize M, Kloner RA, Whittaker P. Regional ischemic 'preconditioning' protects remote virgin myocardium from subsequent sustained coronary occlusion. Circulation. 1993 Mar;87(3):893-9. — View Citation

Wang GL, Jiang BH, Rue EA, Semenza GL. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5510-4. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after on-pump cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	Before starting surgery, is the basal time (Time 0)
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after on-pump cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	2 hours after cardiopulmonary bypass period (Time 1)
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after on-pump cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	8 hours after cardiopulmonary bypass period (Time 2)
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	24 hours after cardiopulmonary bypass period (Time 3)
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after on-pump cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	36 hours after cardiopulmonary bypass period (Time 4)
Primary	Study of time course of HIF-1a plasma levels (absorbance units) after on-pump cardiac surgery	Hipoxia induced factor 1 alpha (HIF-1a) is a transcriptional factor. Arterial blood samples were collected from the radial artery to evaluate the changes in HIF-1a plasma levels induced in patients undergoing on-pump cardiac surgery. Because we think that ischemic post-conditioning (RIP) can increase plasma HIF-1a plasma levels.	48 hours after cardiopulmonary bypass period (Time 5).
Secondary	Study of time course of cardiac marker, Troponin T plasma levels (ng/l)	Troponin T (ng/l) plasma levels. Similar to HIF-1a measures were studied in six arterial blood samples, which were collected from a radial artery line at 6 specific time points (Time 0 to Time 5).	In summary, not repetitive (6 time points): after anesthetic induction, before starting surgery (Time 0) and after cardiopulmonary bypass period, at 2 hrs (Time 1), 8 hrs (Time 2), 24 hrs (Time 3), 36 hrs (T4), 48 hrs (Time 5).
Secondary	Study of time course of cardiac marker, creatine phospho-kinase (CPK) plasma levels (U/l)	Creatine phospho-kinase (CPK) plasma levels (U/l). Similar to HIF-1a measures were studied in six arterial blood samples, which were collected from a radial artery line at 6 specific time points (Time 0 to Time 5).	In summary, not repetitive (6 time points): after anesthetic induction, before starting surgery (Time 0) and after cardiopulmonary bypass period, at 2 hrs (Time 1), 8 hrs (Time 2), 24 hrs (Time 3), 36 hrs (T4), 48 hrs (Time 5).
Secondary	Study of time course of cardiac marker, creatine kinase-MB (CK-MB) plasma levels (ng/ml)	Creatine kinase-MB (CK-MB) plasma levels (ng/ml). Similar to HIF-1a measures were studied in six arterial blood samples, which were collected from a radial artery line at 6 specific time points (Time 0 to Time 5).	In summary, not repetitive (6 time points): after anesthetic induction, before starting surgery (Time 0) and after cardiopulmonary bypass period, at 2 hrs (Time 1), 8 hrs (Time 2), 24 hrs (Time 3), 36 hrs (T4), 48 hrs (Time 5).
Secondary	Study of time course of arterial oxygenation marker (PO2/FiO2), after on-pump cardiac surgery	Oxygenation marker (PO2/FiO2). Similar to HIF-1a measures were studied in six arterial blood samples, which were collected from a radial artery line at 6 specific time points (Time 0 to Time 5).	In summary, not repetitive (6 time points): after anesthetic induction, before starting surgery (Time 0) and after cardiopulmonary bypass period, at 2 hrs (Time 1), 8 hrs (Time 2), 24 hrs (Time 3), 36 hrs (T4), 48 hrs (Time 5).