Safety and Tolerability of Single and Multiple Doses of ISIS 353512 in Healthy Volunteers

Inflammatory Diseases Clinical Trial

Official title:

Double Blind, Placebo-Controlled, Dose-Escalation, Phase 1 Study to Assess the Safety, Tolerability, Pharmacokinetics and Pharmacodynamics of Single and Multiple Doses of ISIS 353512 Administered Intravenously and Subcutaneously to Healthy Volunteers

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT00734240
• Other study ID #: ISIS 353512 CS1
• Status: Completed
• Phase: Phase 1
• First received: August 12, 2008
• Last updated: July 28, 2010
• Start date: July 2008
• Est. completion date: March 2010

Study information

• Verified date: July 2010
• Source: Ionis Pharmaceuticals, Inc.
• Contact: n/a
• Is FDA regulated: No
• Health authority: Canada: Health Canada
• Study type: Interventional

Clinical Trial Summary

The purpose of this study is to evaluate the safety and tolerability of single and multiple doses of ISIS 353512, to evaluate the blood concentration and rate elimination of ISIS 353512 from the blood, and to evaluate the effectiveness of ISIS 353512 in lowering a protein in the blood associated with inflammation.

Description:

This will be a Phase 1, double blind, placebo-controlled, dose-escalation study conducted at a single center to 1)evaluate the safety and tolerability of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously, 2)evaluate the pharmacokinetic profile of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously, and 3)evaluate the pharmacodynamics of ISIS 353512 in lowering constitutive levels of c-reactive proteins in healthy volunteers.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 103
• Est. completion date: March 2010
• Est. primary completion date: February 2010
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Both
• Age group: 18 Years to 55 Years

Eligibility

Inclusion Criteria:

• Age 18 to 55 years

• Male or female although females must be post-menopausal or surgically sterile

• In good health

• BMI < 32 kg/m2

• Give written informed consent to participate in the study

• hsCRP at Screening = 0.50 mg/L and = 5.0 mg/L (for 2 measurements = 2 weeks apart; with difference between measurements = 2.5 mg/L)(Multiple Dose Subjects only, not including dose-titration cohorts)

Exclusion Criteria:

• Pregnant women, nursing mothers or women of childbearing potential

• Clinically significant abnormalities in medical history or physical examination

• Clinically significant ECG abnormalities or vital sign abnormalities (systolic blood pressure < 90 mm Hg or > 140 mm Hg, diastolic blood pressure < 50 mm Hg or > 90 mm Hg or heat rate < 50 or > 100 bpm) at Screening

• Clinically significant abnormalities on laboratory examination, other than hsCRP

• Clinically significant abnormalities in coagulation parameters

• Positive test for HIV, TB (PPD test) or hepatitis B or C at Screening

• History of latent or active tuberculosis or exposure to endemic areas within 8 weeks prior to PPD test at Screening

• History of positive PPD test or positive PPD test result (= 5 mm) indicating possible tuberculosis infection

• Fasting triglycerides > 400 mg/dL at Screening

• Malignancy (with the exception of basal or squamous cell carcinoma of the skin if adequately treated and no recurrence for > 1 year)

• Evidence of ongoing chronic inflammatory condition or infection

• Any other concurrent condition which, in the opinion of the Investigator, would preclude participation in this study or interfere with compliance

• Regular use of alcohol within 6 months prior to Screening (> 14 units of alcohol per week; 1 unit = 150 mL of wine, 360 mL of beer, or 45 mL of 40% alcohol)

• Use of soft drugs (such as marijuana) within 3 months prior to Screening or hard drugs (such as cocaine, phencyclidine [PCP] and crack) within 1 year prior to Screening, or positive urine drug screen at Screening

• Smoking > 10 cigarettes per day

• Use of an investigational drug or participation in an investigational study involving a small molecule with 30 days prior to dosing or participation in an investigational study involving biologic compounds within 90 days prior to dosing

• Regularly taking OTC medications and/or vitamin supplements that may alter CRP, e.g., NSAIDs, aspirin, vitamin E, fish oil, within 14 days prior to Screening

• Receiving prescription medications, including hormone replacement therapy, statins, TNF-a inhibitors, or other anti-inflammatory or immunosuppressive drugs within 30 days prior to Screening

• Immunization with a live attenuated vaccine one month prior to dosing or planned vaccination during the course of the study

• Blood donation of 50 to 499 mL within 30 days of Screening or of > 499 mL within 60 days of Screening

Study Design

Allocation: Randomized, Endpoint Classification: Pharmacokinetics/Dynamics Study, Intervention Model: Single Group Assignment, Masking: Double Blind (Subject, Caregiver, Investigator), Primary Purpose: Treatment

Related Conditions & MeSH terms

• Inflammatory Diseases

Intervention

Drug:
• ISIS 353512
50 mg via 2 hour IV infusion, single dose
• ISIS 353512
50 mg via 2 hour IV infusion, 6 doses over 22 days
• ISIS 353512
50 mg via SC injection, single dose
• ISIS 353512
50 mg via SC injection, 6 doses over 22 days
• ISIS 353512
2 hour IV infusion, 50 mg on day 3, 100 mg on day 5, and 200 mg on day 8
• ISIS 353512
100 mg via 2 hour IV infusion, single dose
• ISIS 353512
200 mg via 2 hour IV infusion, single dose
• ISIS 353512
100 mg via SC injection, single dose
• ISIS 353512
200 mg via SC injection, single-dose
• ISIS 353512
100 mg via 2 hour IV infusion, 6 doses over 22 days
• ISIS 353512
200 mg via 2 hour IV infusion, 6 doses over 22 days
• ISIS 353512
100 mg via SC injection, 6 doses over 22 days
• ISIS 353512
200 mg via SC injection, 6 doses over 22 days
• ISIS 353512
100 mg via SC injection PLUS an oral treatment of a NSAID, acetaminophen, or oral placebo
• ISIS 353512
200 mg via SC injection PLUS an oral treatment of a NSAID, acetaminophen, or steroid
• ISIS 353512
2 hour IV infusion, 50 mg on day 1, 100 mg on day 3, 100 mg on day 5 and 200mg on day 8

Locations

Country	Name	City	State
Canada	Anapharm	Montreal	Quebec

Sponsors (2)

Lead Sponsor	Collaborator
Ionis Pharmaceuticals, Inc.	Anapharm

Country where clinical trial is conducted

Canada, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	To evaluate the safety and tolerability of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously.		14 Days post treatment of each cohort	No
Secondary	To evaluate the pharmacokinetic profile of single and multiple doses of ISIS 353512 administered intravenously and subcutaneously.		14 Days post treatment of each cohort	No
Secondary	To evaluate the pharmacodynamics of ISIS 353512 administered intravenously and subcutaneously.		14 Days post treatment of each cohort	No