Fecal Transplantation for Inflammatory Bowel Disease

Inflammatory Bowel Disease Clinical Trial

— FMT IBD  

Official title:

Fecal Transplantation for Inflammatory Bowel Disease

Clinical Trial Details — Status: Terminated

Administrative data

• NCT number: NCT02391012
• Other study ID #: 0198-12-MMC
• Status: Terminated
• Phase: Phase 1
• First received: March 5, 2015
• Last updated: June 13, 2016
• Start date: April 2015
• Est. completion date: July 2016

Study information

• Verified date: June 2016
• Source: Meir Medical Center
• Contact: n/a
• Is FDA regulated: No
• Health authority: Israel: Ministry of Health
• Study type: Interventional

Clinical Trial Summary

Fecal microbial treatment (FMT) is a treatment that utilizes the microbiota of a healthy intestine as a probiotic preparation. The feces of a healthy individual is fluidized and than inserted into the intestinal tract of a sick individual, assuming that the healthy flora will colonize and cure the intestine. There are reports of the efficacy of this treatment for inflammatory bowel disease but currently the numbers are small.

aim: To investigate whether use of FMT will bring improvement for colitis in IBD patients . Methods: The patients will undergo full colonoscopy, the stool preparation will be infused through the colonoscope during withdrawal of the instrument.

Description:

Back ground: intestinal microbiota have a central role in disease pathogenesis, either in a form of a "permissive" role or as a direct pathogenic cause.

Clostridium difficile colitis; irritable bowel syndrome (IBS) and IBD have all been connected to a disturbance in the equilibrium of intestinal microbiome. But the causative mechanism is not yet known. (5-10) . Various bacteria including lactobacillus acidophilus and bifidobacterium bifidus are being empirically used as treatment for many intestinal ailments. The cause of inflammatory bowel disease in unknown but evidence is gathering that excessive immune reaction of intestinal immune system to microflora combined with a genetic predisposition are responsible for the chronic inflammation.

Fecal microbial treatment (FMT) is a treatment that utilizes the microbiota of a healthy intestine as a probiotic preparation. The feces of a healthy individual is fluidized and that inserted into the intestinal tract of a sick individual, assuming that the healthy flora will colonize and cure the intestine. Previous work had shown success in fecal transplantation as a treatment for clostridium difficile colitis. There are also reports of the efficacy of this treatment for inflammatory bowel disease but currently the numbers are small. A review by Anderson et al describes 17 studies reporting treatment of IBD by FMT. (12). In 9 cases it was done to treat IBD and in 8 cases for intestinal infection in IBD patients. Altogether 41 cases were reported , In some the FMT was inserted through a nasogastric tube directly to the duodenum, in some be colonoscopy and in some by an enema. A significant clinical improvement was reported in 19/25 patients. 13/17 stopped IBD treatment , 15/24 entered full clinical remission. In all 15 patients treated for infection the treatment was successful. No sever adverse effects were reported, Fever was developed in 8 cases and in one case there was exacerbation of colitis after treatment.

Primary aim: To investigate whether use of FMT will bring improvement of at least 2 points in partial mayo score in ulcerative colitis patients, or 70 points in CDAI of patients with Crohn's colitis. One month after FMT.

Improvement will be defined as:

For Ulcerative colitis: a decrease of at least 2 points in the partial mayo score, and a decrease of at least 1 point in endoscopic Mayo score.

For Crohn's disease: A decrease of at least 70 points in Crohn's disease activity index (CDAI) .

Materials and methods:

Study population: 40 patients aged 18-80 years, with histological and endoscopic diagnosis of ulcerative colitis or crohn's colitis who did not respond to either thiopurines or tumor necrosis factor (TNF) inhibitors.

Exacerbation will be defined as partial mayo score higher then 3, with either CRP higher than 0.5 or endoscopic mayo score >1 in ulcerative colitis and CDAI higher them 220 and C reactive protein (CRP) higher than 0.5 in Crohn's colitis.

Stool will be donated by the patients choice either from a relative, preferably a partner to minimize possible transference of an infective agent, alternatively samples will be ordered from "open biom".

Endoscopic disease activity will be defined as follows:

Endoscopic Mayo score:

• Normal or inactive disease: 0

• Mild disease (erythema, decreased vascular pattern, mild friability): 1

• Moderate disease (marked erythema, absent vascular pattern, friability, erosions): 2

• Severe disease (spontaneous bleeding, ulceration): 3

Simple endoscopic score Crohns disease (SES-CD):

Segments of intestine: Rectum,Sigmoid and left colon, Transverse colon,Right colon, Ileum for each segment the Presence of: ulcers,Ulcerated surface, Affected surface, Presence of narrowing, and number of affected segments will be evaluated and the total score sumed, The SES-CD will than be calculated as follows: TOTAL - 1.4 X (number of affected segments) = E-CDI

Inclusion criteria

Donor:

1. Signed informed consent

2. Basically healthy, no hospitalization in the last 6 months, no active malignant disease (with the exclusion of Basal cell skin tumor that was treated).

3. No antibiotic treatment in the last 6 months

4. Preferably a partner of the patient, or a relative leaving in the same house

5. No febrile illness in the last month

6. Normal laboratory tests including: blood count, liver and kidney function, negative hepatitis A immunoglobulin M (IgM), negative hepatitis C antibody, negative HbsAg, negative HIV , human T-lymphotropic virus (HTLV I/II), negative venereal disease research laboratory ( VDRL).

7. Stool tests: Negative stool culture, negative C. difficile toxins (A and B). Negative parasite microscopy. Fecal calprotectin.

Patient:

1. Signed informed consent

2. Inflammatory bowel disease diagnosed at least 3 months ago

3. Failure of either one immunomodulator of at least 3 months duration, or TNF inhibitor full induction treatment, or intolerance to either of these drugs.

4. Currently active disease, partial Mayo score ≥4 for ulcerative colitis (UC), or CDAI ≥200 for Crohn's disease (CD).

5. negative HIV , HTLV I/II, negative stool culture, Negative C diff toxin, negative cytomegalovirus ( CMV)

Treatment protocol:

1. Both donor and recipient will sign informed consent

2. Both donor and recipient will do the above mentioned laboratory tests, the investigator will verify that the results are negative.

3. Recipient will be prepared for colonoscopy with 3 liters of polyethylene glycol according to the routine protocol.

4. The donor will bring same day stool, alternatively, preparation from "open biom" will be used.

5. 200 gr stools will be mixed with sterile 200ml sodium chloride (NACL) 0.9% and then filtered to exclude large particles.

6. The patients will undergo full colonoscopy, the stool preparation will be infused through the colonoscope during withdrawal of the instrument.

7. If no improvement within 7 days, the patient will be offered the option of another FMT using enema prepared in a similar manner.

8. Patients will be instructed to maintain a high fiber diet,

Follow up:

On first colonoscopy:

Exact description of the length of the involved segment + Mayo visual score for UC and simple endoscopic score - CD (SES -CD) for Crohn's disease.

2 biopsies from each colonic segment: Rt colon, transverse colon, left colon, rectum.

Weekly partial Mayo score or Harvey Bradshow Index (HBI ) in the first month, then once a month for 6 months.

After 6 months repeat colonoscopy + biopsies + stool samples. Colonoscopy does not have to be complete but has to include the segment that was inflamed in the first colonoscopy.

At the time of colonoscopy blood tests including complete blood count (CBC), liver and kidney function, CRP, fecal calprotectin.

Microbiota profiles:

1. From both the donor and the recipient before FMT.

2. From the recipient: one month and 6 months after FMT. Stool samples will be frozen immediately at -20° and then transferred to the laboratory for microbiota analysis.

Recruitment information / eligibility

• Status: Terminated
• Enrollment: 9
• Est. completion date: July 2016
• Est. primary completion date: June 2016
• Accepts healthy volunteers: No
• Gender: Both
• Age group: 18 Years to 80 Years

Eligibility

Inclusion Criteria:

1. Signed informed consent

2. Inflammatory bowel disease diagnosed at least 3 months ago

3. Failure of either one immunomodulator of at least 3 months duration, or TNF inhibitor full induction treatment, or intolerance to either of these drugs.

4. Currently active disease, partial Mayo score =4 for ulcerative colitis, or CDAI =200 for CD.

5. negative HIV , HTLV I/II, negative stool culture, Negative C diff toxin, negative CMV

Exclusion Criteria:

1. No informed consent

2. Non active inflammatory bowel disease.

3. Active infection in either the donor or the recipient,

Study Design

Endpoint Classification: Efficacy Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment

Related Conditions & MeSH terms

• Inflammatory Bowel Disease
• Inflammatory Bowel Diseases
• Intestinal Diseases

Intervention

Biological:
• Fecal microbial l transplantation
infusion of preparation of fecal preparation from a healthy donor to a colitis patient

Locations

Country	Name	City	State
Israel	Meir hospital	Kefar Saba
Israel	Gastroenterology institute Meir Hospital	Kfar Saba

Sponsors (1)

Lead Sponsor	Collaborator
Meir Medical Center

Country where clinical trial is conducted

Israel, 

References & Publications (1)

Anderson JL, Edney RJ, Whelan K. Systematic review: faecal microbiota transplantation in the management of inflammatory bowel disease. Aliment Pharmacol Ther. 2012 Sep;36(6):503-16. doi: 10.1111/j.1365-2036.2012.05220.x. Epub 2012 Jul 25. Review. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Improvement of disease activity index.	: a decrease of at least 2 points in the partial mayo score, and a decrease of at least 1 point in endoscopic Mayo score.; For Crohn's disease: A decrease of at least 70 points in CDAI.	4 weeks	No
Secondary	improvement of endoscopic score	Improvement of at least one point in endoscopic score	4 weeks	No
Secondary	Maintenance of remission/ improvement during follow up	Maintenance of remission/ improvement during follow up	6 months	No
Secondary	Patients perspective of the treatment (do patients regard the treatment as acceptable)	do patients regard the treatment as acceptable	6 months	No
Secondary	Cost estimation of the treatment	cost of treatment	6 months	No
Secondary	side effects (presence of fever,or rise of 50% or more in the number of bowel movements)	monitoring side effects of treatment,specifically the presence of fever above 38? c or a rise of 50% or more in the number of bowel movements during the first 3 days after the infusion	6 months	Yes