Hyperreactive Airway Clinical Trial
Official title:
Evaluating the Capacity of High-sensitivity Serum CRP Levels to Predict Bronchial Hyper Responsiveness in School Age Children
Background:
Bronchial hyper responsiveness (BHR) assessed by methacholine challenge test (MCT) may aid
in the diagnosis of asthma, while negative MCT can help to exclude the diagnosis. Laboratory
measures that predict the results of MCT are expected to reduce the number of procedures.
The possible capacity of High sensitive C-reactive protein (hs-CRP), a marker of systemic
inflammation, to predict negative or positive MCT in children has not been evaluated.
Aim: to evaluate the capacity of hs-CRP to predict positive or negative MCT in school aged
children and to compare it with markers of airway inflammation: Fractional exhaled Nitric
Oxide (FeNO) and markers of allergic sensitization (IgE and peripheral blood eosinophils).
Design: Prospective study evaluating these parameters in patients with positive and negative
MCT.
Participant selection: The study population included subjects (age range 6 to 18 years)
referred for Methacholine Challenge Test (MCT)
Sample size: 130 participants in the two groups. Intervention: Each subject will undergo
evaluation including a respiratory questionnaire, methacholine challenge test with
determination of PC20(the provocative concentration that reduced FEV1 by 20% from baseline),
exhaled nitric oxide (eNO). Venous blood will be analyzed for complete blood count +
eosinophils, IGE levels, hs-CRP. All measurements will be evaluated in a single 3 hour
visit, with no follow up study visits.
Primary end point: hs-CRP levels as add on tool to predict negative or positive MCT in
children Secondary outcome parameters: All other parameters are the secondary end points.
n/a
Observational Model: Cohort, Time Perspective: Prospective