Antioxidant in Patients HIV+ Supplemented With Microencapsulated of Red Pomegranate

HIV Positive Clinical Trial

Official title:

Antioxidant Activity and Lipid Peroxidation Status in Patients HIV+ Supplemented With Microencapsulated of Red Pomegranate

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03531749
• Other study ID #: FSSA2016051
• Status: Completed
• Phase: N/A
• Start date: January 2017
• Est. completion date: May 2017

Study information

• Verified date: May 2018
• Source: Universidad Autónoma del Estado de Hidalgo
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Human immunodeficiency virus (HIV) infection continues to be a pandemic, Mexico has around 184,000 people infected by this virus. A common metabolic problem for these patients is oxidative stress (OS), which has been related with the progression of the disease and the presence of comorbidities. Pomegranate is a fruit rich in antioxidants, which potentially can inhibit or reduce deleterious metabolic compounds resulting from OS; however; it has never been tested in patients infected with HIV. The present project was done in patients HIV+ from state of Hidalgo in order to see the effects of microencapsulated red pomegranate juice (MRPJ) and ascorbic acid (AA) on antioxidant activity and lipid peroxidation both biomarkers of oxidative stress. Sixty subjects were recruited, 30 HIV positive (HIV+) and 30 HIV negative (HIV-). Three subgroups (n=10) were formed from each group: 1) supplemented with (1g/d) MRPJ; 2) supplemented with 1g/d AA; and 3) control group (unsupplemented). The intervention lasted 90 days and blood samples were taken four times: at the beginning and every 30 days. Antioxidant activity in the blood serum was measured by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS + (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) methods while lipid peroxidation by malondialdehyde (MDA) levels which was measured by TBARS method. The baseline results showed a significant decrease of antioxidant activity in HIV+ groups compared to the HIV- groups, although there was no significant difference in lipid peroxidation, as measured by MDA assay levels. Several studies suggest that the reduction of antioxidant activity is a consequence of the infection and the antiretroviral treatment, although the organism tries to reestablish it unbalance it usually fails, thus (OS) is significant in these patients. The groups that received AA had antioxidant activity greater than the MRPJ treated. MRPJ treatment, however, the groups that received MRPJ had significantly reduced lipid peroxidation. Reduced lipid peroxidation could have more beneficial effects on HIV+ subjects since the reduction of markers of OS, such as lipid peroxidation, has been associated with reductions in the risk of death from HIV.

Description:

Since Patients HIV+ suffer oxidative stress, microencapsulated of red pomegranate juice will be evaluated for decreasing oxidative stress in HIV+ patients it will be compared with patients treated with ascorbic acid. The supplementation will take 90 days and blood samples were taken four times: at the beginning of the study and every 30 days thereafter. Antioxidant activity in the blood serum was measured by the DPPH and ABTS+ methods lipid peroxidation was measured as stress oxidative markers

Recruitment information / eligibility

• Status: Completed
• Enrollment: 60
• Est. completion date: May 2017
• Est. primary completion date: May 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion criteria:

• Over 18 years

• Having a positive HIV diagnosis

• Having antiretroviral treatment

• Status of one of the following clinical categories A1, A2, B1 and B2 at the time of the study start,

• Residents of the city of Pachuca

Exclusion Criteria:

• Subjects taking antioxidants

• Pregnant women.

• Subjects taking of angiotensin-converting enzyme inhibitors (e.g.captopril or enalapril).

• subjects who do not comply with at least 95% of the adherence of the supplementation

Related Conditions & MeSH terms

• HIV Positive
• HIV Seropositivity

Intervention

Dietary Supplement:
• HIV+ MRPJ
supplemented with (1g/d) MRPJ
• HIV+ ascorbic acid
Supplemented with (1g/d) AA

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Universidad Autónoma del Estado de Hidalgo	National Council of Science and Technology, Mexico

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Biochemical analyses to determine antioxidant capacity	Four different tests for antioxidant activity were utilized. Plasma antioxidant capacity, catalase, H2O2, and TBARS	At 0 time (baseline)
Secondary	Biochemical analyses to determine antioxidant capacity	Four different tests for antioxidant activity were utilized. Plasma antioxidant capacity, catalase, H2O2, and TBARS	30 days after intervention