Clinical Trial Details
— Status: Enrolling by invitation
Administrative data
| NCT number |
NCT03666052 |
| Other study ID # |
SCALE-HM |
| Secondary ID |
|
| Status |
Enrolling by invitation |
| Phase |
|
| First received |
|
| Last updated |
|
| Start date |
September 16, 2018 |
| Est. completion date |
September 2038 |
Study information
| Verified date |
September 2018 |
| Source |
Shanghai Eye Disease Prevention and Treatment Center |
| Contact |
n/a |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Observational
|
Clinical Trial Summary
1.1 Research objectives A．To observe the fundus changes in the posterior pole (morphology,
thickness, asymmetry, blood flow density, etc) with the myopia progression.
B．To observe morphological changes in choroid and peripheral region of retina with myopia
progression.
C. To observe changes of visual function (contrast sensitivity, Microperimetry, etc) with
myopia progression.
D. To detect the susceptibility genes related to high myopia and myopic fundus changes; to
test the levels of Vitamin D, riboflavin, transforming growth factor（TGF）, insulin-like
growth factor（IGF）, fibroblast growth factor（FGF）, etc.
E. To observe the changes of living quality, psychology, behavior and social activities of
high myopic children.
1.2 Research design Prospective cohort study. After completing the baseline survey, the
planned follow-up frequency is once a year.
1.3 Research cycle 2018.06~2038.06 (at least).
1.4 Expected results A． Registration completed a study of high myopia research for children
and adolescents covering around 3,000 people; B． Establish a database information management
system and workflow SOP（standard operating procedure）file for the study of high myopia
registration in children and adolescents; C． Further clarify the changes in the retinal,
choroidal and scleral tissue structures, blood flow density, etc. in the macular area and the
optic disc; D． Revealing the changes of the retina, choroid and other tissues in the
peripheral area with the progression of myopia; E． To clarify the relationship between
changes in the fundus structure and changes in visual function in the posterior pole; F．
Further clarify the etiology and pathogenesis of high myopia, pathological myopia and myopic
fundus lesions, and identify the relationship between high myopia and pathological myopia; G．
From the perspectives of society, behavior and psychology, the effects of high myopia and
pathological myopia on children and adolescents will be fully demonstrated.
2. Research object 2.1 General characteristics of the research object
Based on the refraction development archive system that has been constructed in Shanghai, the
list of children and adolescents with high myopia was selected from the database of
children's refractive development archives information in Shanghai. Children of different
ages with high myopia must meet the following conditions:
1. 4-5 years old, equivalent spherical error(SE) ≤ -4.0 diopter(D）;
2. 6-8 years old, equivalent spherical error(SE) ≤ -6.0 diopter(D）;
3. 9-18 years old, equivalent spherical error(SE) ≤ -8.0 diopter(D）.
2.2 Sample size A total of 1.25 million children and adolescents are currently registered,
4,006 (0.32%) of which meet the entry requirements. Among the 4~5 year olds, there are 815
people with SE≤-4D; 842 people with SE≤-6 D among the 6~8 year olds; 2349 people with SE≤-8D
among the people aged 9 and over . Taking into account the 50% non-response and the
proportion of the exclusion, the initial registration number is about 2,000.
2.3 Source of study object Children and adolescents who meet the inclusion criteria in the
Shanghai Children's Refractive Development Archives Information Database System.
Description:
1. introduction 1.1 Overview of myopia and high myopia Myopia is the most common eye
disease in the world . Among them, the incidence and prevalence of myopia are highest in
developed countries and regions in East and Southeast Asia. It is predicted that by
2050, global myopia patients would reach 5 billion, 1 billion of which were high myopia.
In developed countries and regions in East and Southeast Asia, the prevalence of myopia
in high school graduates is as high as 80% to 90%. A study in Taiwan showed that more
than 80% of adolescents suffered from myopia when they graduated from high school, 10%
of whom were high myopia. Another study which focused on university students in Shanghai
showed that 95.5% of college students suffered from myopia, and 19.5% of them were high
myopia. In stark contrast, the prevalence of myopia in the same age in western developed
countries is between 20% and 40%. In less developed countries and regions of the world,
the incidence of myopia in young people is about 5% to 10% due to the low level of
education.
At present, the etiology and pathogenesis of myopia and high myopia are still unclear.
Environmental and genetic factors are involved in the development of myopia and high
myopia. Among them, the level of education and outdoor activity time are closely related
to the development of myopia and high myopia. At present, most studies believe that the
higher the education level, the higher the incidence of myopia and the deeper the degree
of myopia. In addition, most studies suggest that outdoor activities was a protective
factor for myopia, and increasing outdoor activity time could reduce the incidence of
myopia. Also, some studies have shown that the age of onset of myopia was closely
related to high myopia. Furthermore, genes may also be closely related to the occurrence
of myopia and high myopia. At least 19 myopic loci have been identified through family
studies and twin studies till now. With the completion of the Human Genome Project,
genome-wide association analysis technology is becoming more and more mature, and more
and more related genes and mutation sites have been elucidated, but the pathogenic genes
of myopia are still not completely clear. In addition, the genes related to pathological
myopia and fundus lesions are currently unclear. In recent studies, high myopia and
pathological myopia are not strictly differentiated (pathological myopia is defined by
diopter or axial length alone). Therefore, genes related to pathological myopia need
further research, in order to judge whether high myopia and pathological myopia are two
different diseases at genetic level or different states controlled by the same genes.
1.2 Relationship between high myopia and pathological myopia The fundus of patients with
high myopia is often accompanied by a series of pathological changes, such as posterior
scleral staphyloma, retinal choroidal atrophy, lacquer crack, choroidal
neovascularization（CNV）, macular hemorrhage, Fuchs plaque, retinal palpebral fissure,
retinal tear, retinal detachment. These pathological changes are important causes of
decreased vision and even blindness. Some studies have shown that macular degeneration
caused by myopia has become a major cause of blindness and low vision. In Jing'an
District of Shanghai, high myopia with macular degeneration has leapt to the first place
in adult blindness. A study in Beijing showed that pathological myopia has become the
leading cause of blindness and visual impairment in the 40-49 age group.
Although high myopia is closely related to pathological myopia, and most patients with
pathological myopia are highly myopic, high myopia is not equivalent to pathological
myopia. The change of diopter and axial length do not fully reflect the characteristics
of pathological myopia. Some patients with high myopia have no definite fundus lesions,
and some non-high myopia patients may also have posterior scleral staphyloma,
retinochoroid atrophy and other complications. At present, Ohno-Matsui K et al define
pathological myopia as: myopic macular degeneration ≥ 2 (diffuse retinochoroid atrophy),
or additional lesions (lacquer cracks, choroidal neovascularization, Fuchs plaque), or
posterior scleral staphyloma. In this definition of pathological myopia, we focus on
fundus lesions caused by myopia, and do not emphasize diopter and axial length.
At present, there are still many unknowns about the relationship between high myopia and
pathological myopia. The process of developing simple high myopia in children and
adolescents as pathological myopia is still unclear. Does high myopia eventually evolve
into pathological myopia? Are pathological myopia and high myopia different stages of
the same disease or two different diseases? Which fundus changes can predict the
occurrence of pathological myopia? These problems need further exploration.
1.3 Morphology and visual function changes in fundus of children with high myopia At
present, there are few studies on the changes of fundus morphology and visual function
in children with high myopia. And long-term follow-up studies are also lacking.
Morphological studies suggest that the common fundus changes in children with high
myopia include β-peripapillary atrophy（β-PPA）, optic disc tilt, etc., but the incidence
of posterior scleral staphyloma and retinochoroid atrophy is low in childhood.
Furthermore, some studies have suggested that diffuse retinochoroid atrophy around the
optic disc in childhood could easily develop into pathological myopia in adulthood. In
addition, thinning of the retina, choroid, and sclera is also present in children with
myopia and high myopia. In addition to the fundus of the posterior pole, there are also
multiple lesions in the peripheral fundus. Some studies considered that peripheral
fundus lesions such as lattice-like degeneration, non-compressive whitening, and retinal
tears are more common in children with high myopia.
Corresponding to structural changes, there are also changes in micro-field and
electrophysiology of patients with high myopia. However, there is few studies focusing
on children and adolescents with high myopia, and no long-term follow-up studies has
been reported. There are still many unknowns in the early morphological changes,
evolution process and visual function of high myopia, which deserve further exploration.
1.4 The direction of this research A． Follow-up study of morphological changes in the
fundus of children with high myopia; B． Follow-up study of visual function changes in
children with high myopia; C． Etiological studies related to high myopia in children; D．
Follow-up study of quality of life, psychology, behavior, and social interactions in
children with high myopia.
2. Data collection 2.1 Preparatory work A． Establish Shanghai Children and Adolescents High
Myopia Registration Information System and Workflow: a set of information function
modules including appointment, real-time collection of exam data, online feedback of
exam results, daily consultation contact and data management analysis. And then
establish the workflow SOP file; B． Train physicians, optometrists and other relevant
staff responsible for the research; C． Print promotional materials, questionnaires,
informed consent forms and examination flow charts; D． Contact the children and
adolescents in the Shanghai Children's Refractive Development Archives Information
Database System through their school and use science lectures, WeChat（a kind of APP）
official accounts, Weibo(Chinese version of Twitter), paper leaflets to promote the harm
of high and pathological myopia to children, adolescents and their parents, as well as
introduction of the content of this study, the participants' benefits and potential
risks; E． Sign an informed consent form to collect information on the participants; F．
The appointment is registered through the information system.
2.2 Examination process The brief process is as follows: Identity information
registration > height, weight > naked eye vision & wearing vision > axial length >
intraocular pressure > slit lamp anterior segment examination > Microperimetry
(selected) > cycloplegia > Autorefraction and subjective refraction > Pentacam,
Swept-source optical coherence tomography（SS-OCT，Topcon), wide-angle optical coherence
tomography angiography(OCTA,Zeiss-9000), fundus color photography +
autofluorescence(Topcon), Ultra wide Angle fundus photography(Optos), mfERG (optional),
wavefront aberrations > blood sample collection / saliva specimens > fill out the
questionnaire.
The specific flow chart is as follows.
2.3 Ophthalmology inspection project operation rules 2.3.1 Vision test The eyesight
examination used the ETDRS visual acuity chart (LCD backlit lamp, WH0701), the test
distance was 4 meters, and the visual target at 20/20 was the same height as the eye of
the examinee. The recognition time of each visual target is 2~3s; the eye of the subject
is required to be opened normally for examination, and the blinking, hoeing, neck
stretching and peeking are strictly prevented. Vision is converted to a decimal count
record. Visual acuity examination includes two parts: uncorrected visual acuity (UCVA)
and corrected visual acuity (CVA). Children who are not wearing glasses are only
examined by UCVA, and children who wear glasses are required to check CVA after
completing the UCVA test.
2.3.2 Axis measurement The axial measurement was performed using an IOL Master (version
5.02, Carl Zeiss Meditec, Germany). Simulated eye calibration was needed before
measurement. Each eye was measured repeatedly for 3 times, and the difference was less
than 0.02 mm each time. For those who still have large fluctuations in multiple
measurements, the examiner needs to record it.
2.3.3 Intraocular pressure measurement Intraocular pressure was measured using a
non-contact tonometer (NT-4000, Nidek, USA). Each eye was repeatedly measured 3 times
and averaged, and the difference between each two was less than 5 mmHg. Those with an
intraocular pressure higher than 24 mmHg should be recorded and added for visual field
examination.
2.3.4 Ophthalmologist examination Ophthalmologist examinations included anterior segment
slit lamp examination (66 Vision. Tech, Suzhou,China) and ophthalmoscopy examination(66
Vision. Tech, Suzhou,China). The examination of the anterior segment slit lamp includes
the eyelid, conjunctiva, cornea, anterior chamber, iris, pupil, lens and anterior
vitreous body, which is completed by the investigator's senior ophthalmologist. For
patients with peripheral anterior chamber depth less than 1/2 corneal thickness, or
acute inflammation of the anterior segment of the eye and other related diseases, it is
not suitable for cycloplegia afterwards. It should be registered by the examining
physician and suspended or excluded from the study. In addition, the ophthalmologist
should use corneal reflection, occlusion-de-covering, etc. to determine whether the
subject has strabismus (hidden strabismus or strabismus). Those who were strabismus
should be excluded from the study. For those suspected of having fundus diseases,
ophthalmoscopy (direct ophthalmoscope or 90D) can be performed after cycloplegia, and
the nature of fundus disease is judged by an ophthalmologist. Participants with
myopia-related fundus lesions can be registered and included in the study, while
participants with other fundus lesions need to be excluded after recorded. Those who had
other organic eye diseases were also excluded after recording.
2.3.5 Microperimetry Microperimetry examinations were performed in the darkroom and
needed to be performed prior to OCT and fundus photographic examinations to avoid
influence by bright light. Microperimetry was performed using an MP1 micro-perimeter
(MP-1, Nidek, Japan), and the Goldman III, 4-2-1 mode was selected to detect retinal
light sensitivity within 10 degrees of the macula. There were a total of 40 stimulation
points (1°-8 stimulation points, 3°-16 stimulation points, 5°-16 stimulation points),
and the stimulation intensity of each point is from 20 decibels (dB) (equivalent to 20
asb) to 0 dB ( Equivalent to 400 asb) change. Initial stimulation intensity is 16 dB and
stimulation duration is 100 ms. The background light is white, 4asb. All participants
will receive at least 5 light stimuli before the formal test, making them familiar with
the examination process and minimizing the impact of learning effects. The test
procedure is as follows: the infrared fundus camera takes a fundus shot (45° field of
view) and transmits it to the video monitor. The fixation target and the stimulation
point are projected onto the retina by the liquid crystal display. The eyeball automatic
tracking system tracks the position of the retina in real time to ensure each
stimulation point in the predetermined retinal position. Every 60 s system will project
a super-threshold stimulus on the physiological blind spot to monitor the false positive
reaction. If a false positive reaction occurs, it needs to be re-examined. This item is
optional for children under 10 years old.
2.3.6 Cycloplegia The cycloplegia procedure is as follows: 1 drop of 0.5% proparacaine
(Alcaine, Alcon) is added to the conjunctival sac of each eye, and 2 drops of 1%
cyclopentolate are added to each eye after 15 seconds. (Cyclogyl, Alcon), 5 minutes
apart. After each drip, ask the participant to press the inner canthus for a few seconds
gently and try to take the head back posture. The last drop of cyclopentolate was
instilled into the conjunctival sac for 30 minutes to check for light reflection. If the
light reflection disappeared and the pupil diameter was greater than 6 mm, cycloplegia
was considered complete. If the light reflection still exists, add a third drop of
cyclopentolate, and re-examine the light reflection and pupil diameter after 20 minutes.
If there was still light reflection, the inspector needs to record this. During
cycloplegia, if the participant has symptoms of ocular discomfort, the ophthalmologist
should carefully examine it and give an appropriate treatment.
2.3.7 Measurement of refractive state and corneal radius of curvature (CR) The
refractive status and CR measurements were performed using an automated computer
refractometer (KR-8900, Topcon, Japan), which was performed after ciliary muscle
paralysis. Simulated eye calibration needed before measurement. Each eye was repeatedly
measured three times to average, and if any two results differ by more than 0.5 D, the
measurement needed to be repeated. If there were still significant differences in the
results of multiple measurements, the examiner needed to record it.
2.3.8 Subjective optometry Children who do not wear glasses have a UCVA of less than 0.8
(less than 0.63 for children 6 years of age and younger) or children with glasses have a
CVA of less than 0.8 (children under 6 years of age and below 0.63) need to finish a
subjective optometry after cycloplegia in order to measure the best corrected visual
acuity (BCVA). If the BCVA is less than 0.8 (less than 0.63 for children aged 6 and
under), or the degree of compliance during the examination is poor, the examiner should
record it. If the BCVA is less than 0.8 (children under 6 years of age and below is less
than 0.63), further examination by an ophthalmologist is required to determine whether
the inclusion conditions are still met.
2.3.9 Pentacam Pentacam (OCULUS Optikgeratic Gmbh, Germany) was examined after
cycloplegia. Measurements include corneal diameter and curvature, anterior chamber depth
and volume, anterior chamber angle, pupil diameter, crystal thickness, etc. Shooting
requirements: Image quality display "OK", crystal thickness value is available. This
item is optional for children under 6 years old.
2.3.10 SS-OCT （Topcon） SS-OCT (DRI OCT Triton, Topcon, Tokyo, Japan) was examined after
cycloplegia. OCT location: Macular + optic disc area. Shooting mode: 12*9 mm 3D scan
mode (4 overlap) / Line scan (64 overlap) + 9 mm radial scan mode (16 overlap, follow up
mode) + optic disc area 9 Mm radial scan mode (16 overlap, follow up mode) + macular
area 7*7 mm 3D scan mode (4 overlap) + optic disc area 6*6 mm 3D scan mode (4 overlap).
Shooting requirements: input spherical error, cylinder, axis length, corneal curvature
radius correction magnification before shooting; image signal of strength 3D scan mode
is not less than 50, radial scan mode is not lower than 60, peripheral image avoids
mirror flip as much as possible. When shooting the disc area, we need to manually adjust
the shooting center to the center of the disc. If the image quality is affected by
blinking or eye movement during shooting, you need to re-shoot. If you still can't meet
the requirements, you need to record it. SS-OCT comes with a fundus color photograph.
The shooting position is required to be consistent with the SS-OCT scanning position and
to avoid eyelids, eyelashes and hair occlusion. Dark areas are avoided in the image, and
the image quality is not less than 90. This item is optional for children under 6 years
old.
4.3.11 Wide angle OCT/OCTA (Zeiss-9000) Wide-angle OCT/OCTA examinations were performed
after cycloplegia.OCT/OCTA location：macular + optic disc area. Shooting mode: 12*12mm,
15*9mm angio mode; 16mm loop sweep mode (horizontal and vertical directions) a total of
8 scans. Shooting requirements: communicate more with the subject;the forehead and chin
must be close to the instrument.If the image quality is affected by blinking or eye
movement during shooting, you need to re-shoot.This item is optional for children under
6 years old.
2.3.12 Fundus photography + autofluorescence Fundus photography + autofluorescence was
performed after cycloplegia.Shooting content: pseudo color eye fundus + spontaneous
fluorescent fundus.Shooting requirements: The exposure intensity is 30ws and 300ws
respectively and it needs to focus on the split line and rotate the upper refractive
compensation button. If you find the participant not completely cycloplegic, please take
another drop of cyclopentolate , otherwise there will be a dark area. This item is
recommended for final inspection as the exposure is too bright.
2.3.13 Ultra wide-angle fundus photography (Optos) Optos ultra-wide-angle fundus
photography was performed after cycloplegia. Shooting content: pseudo color eye fundus +
spontaneous fluorescent fundus. Shooting requirements: the same as color fundus
photography.
2.3.14 mfERG mfERG (RETIscan, 3.15 version, Roland, Germany) was examined after
cycloplegia. After 0.5% topical anesthesia with procaine hydrochloride, the contact lens
was placed on the cornea, the ground electrode was placed in the middle of the forehead,
and the reference electrode was placed on the lateral iliac crest to correct refractive
error. The stimulator has an average brightness of 102 cd/m 2 (4~200 cd/m 2), a contrast
ratio of 99%, and stimulates the retina with a center of about 30° around the macula.
There are 61 hexagons, each of which is six. The edge is flipped in black and white
under the control of the binary m sequence. Each stimulation cycle time is 47 s,
stimulating 6 cycles. This item is optional for children under 10 years old.
2.3.15 Wavefront aberration When the corneal topography and wavefront aberrations are
measured, the subject's eyes are enlarged, and the operator or assistant pulls the upper
eyelid if necessary.
2.4 Collection of blood/saliva specimens 5 ml of Blood/saliva specimens were collected.
Blood/saliva specimens are temporarily stored in a portable refrigerator on site and
transferred to a -80 °C deep-temperature refrigerator as soon as possible. The detection
of serum riboflavin and related cytokine levels will be carried out in the Ophthalmology
Laboratory of the Shanghai General Hospital affiliated to Shanghai Jiao Tong University.
2.5 Questionnaire
The questionnaire is divided into 5 parts:
A． General information, including birth status (maternal pregnancy status, premature
birth history, feeding status, etc.), family status (family history of myopia, family
economic status, parental education level, parental physical condition, etc.), growth
and development status, living and learning status (living environment , dietary status,
sleep status, academic burden, academic performance, etc.), past medical history
(including systemic and eye diseases); B． Quality of life for children with high myopia
(NEI-VFQ-25 scale); C． High myopia children's psychology (NEI-VFQ-25 scale, depression
self-assessment(SDS), anxiety self-assessment table(SAS), self-esteem scale(SES)); D．
High myopia child behavior (NEI-VFQ-25 scale); E． Social interaction for children with
high myopia (NEI-VFQ-25 scale). The questionnaire is completed by the parent or
guardian. Children and adolescents aged 10 or older are required to complete additional
questionnaires to conduct self-evaluation on quality of life, psychology, behavior and
social interaction. The questionnaires are mainly filled in online. For parents or
children who are not convenient to fill out the questionnaire online, a paper-based
questionnaire can be issued and collected after completion.
3. Organization and quality control of research 3.1 Drafting, revision and finalization of
the plan A. The program was drafted by the Shanghai Eye Disease Prevention and Treatment
Center. The draft is reviewed by the expert group, and the draft is discussed, revised,
and finalized in the form of a seminar. The research plan was implemented after approval
by the ethics committee.
B. In the process of research implementation, if major revisions to the research plan
are required, they must be submitted to the ethics committee for approval before further
implementation.
3.2 Research institute A. The Shanghai Municipal Health Bureau and the Shanghai
Municipal Education Bureau are responsible for the coordination of major issues.
B. Shanghai third-level eye disease prevention and control system (city-district
(county)-community) jointly guarantees the implementation of research C. The Shanghai
Eye Disease Prevention and Treatment Center is the only municipal-level eye disease
prevention and control institution in Shanghai. It leads the city's 16 districts and
counties and more than 240 community-level eye care institutions to jointly develop eye
care services.
D. The Shanghai Eye Disease Prevention and Treatment Center can provide sufficient human
resources for the research. Most of the inspection teams are staff members of the
hospital and have extensive clinical experience. In addition, the Shanghai Eye Disease
Prevention and Treatment Center has extensive experience in recruiting temporary
volunteers.
3.3 Quality control and supervision A． All participants in the study are required to be
trained before the study is officially launched. The training content is printed as a
work manual and distributed to researchers. The clinician, optometrist or technician who
participates in the examination needs to learn the corresponding part of the work
requirements, procedures, instrument use and operation specifications before going to
work. After the passing examination, the official examination can be carried out. For
items that are responsible by multiple people, it is necessary to complete the
consistency test before the study is officially carried out to ensure that the results
between different examiners are comparable.
B． All inspectors are only able to know the relevant parameters necessary for the
inspection items they are responsible for, and remain blind for other irrelevant
parameters.
C． The instrument should be calibrated before the start of the work on each inspection
day.
D． Researchers responsible for quality control should regularly check the inspection
data of each inspection post. The quality control standards refer to the operating rules
on the work manual. For those who have higher failure rate, they need to re-train and
pass the examination before they can re-employ.
E． Follow-up content and criteria were consistent with baseline. The equipment for
follow-up examination is as close as possible to the baseline, and the examiner is as
consistent as possible. If inspection instrument must be replaced due to the upgrade,
the comparability of the old and new instruments should be evaluated.
F． The follow-up interval was as consistent as possible. G． The reliability and validity
of the questionnaire needs to be assessed before use.
3.4 Data entry, analysis and deletion A． The online data system is researched and
developed by professional information technology companies, used to check real-time data
entry, questionnaire filling, etc. The accuracy and completeness of the data is
automatically determined by the data system.
B． The entry of non-electronic data is done independently by two people. C． Data
deletion and analysis were performed independently by two statisticians.
4. Statistical Analysis 4.1 Descriptive statistics A． Continuity variables: sample size,
mean, standard deviation, minimum, maximum, quartile B． Classification variable or grade
variable: frequency distribution
4.2 statistical methods A. Continuous variables: The normality test uses the
Kolmogorov-Smirnov test. If the normal distribution is satisfied, the t test or one-way
ANOVA is used; if the normal distribution is not satisfied, the Mann-Whitney U test or
the Kruskal Wallis test is used. The two-two comparison between groups was performed
using the Bonferroni method.
B. Categorical variable: chi-square test. C. Correlation analysis: simple linear
regression and stepwise multiple linear regression, using nonlinear regression if
necessary.
4.3 Statistical significance All differences were statistically defined as P < 0.05
(bilateral)
4.4 Subgroup analysis Subjects will be assigned to different subgroups for subgroup
analysis based on age, gender, diopter, axial length, fundus structure, and functional
changes.
4.5 Interim analysis Baseline data (2018) and follow-up data for every 3 years are used
for analysis and reporting.
5. Ethical issues 5.1 Ethics committee The study will be carried out after the approval of
the Shanghai General Hospital Ethics Committee and strictly abide by the Helsinki
Declaration.
5.2 Protect the privacy of participants In order to protect the privacy of the children
and adolescents in the test, when the research materials are provided to other
organizations, the subject code or initials should be used instead of their ID number or
real name. In addition, the researcher and the relevant staff involved in the research
must keep the privacy information of the children and adolescents in the test
confidential.
5.3 Informed consent The informed consent form will be distributed to the children and
adolescents participating in the study and their parents or guardians after approval by
the ethics committee. For children and adolescents and their parents or guardians who
voluntarily agree to participate in the study, parents or guardians are required to sign
an informed consent form prior to the baseline study. Before signing the informed
consent form, the researcher should fully introduce the content of the study, the
benefit of the subject and the potential risks, and confirm that the children and
adolescents and their parents or guardians fully understand and voluntarily sign the
informed consent form. When signing the informed consent form, the parent or guardian
has sufficient time to consider and has the right to ask questions. For questions from
parents or guardians, the researcher needs to provide adequate answers. For parents or
guardians who agree to sign an informed consent form, the investigator signs the
informed consent form after the informed consent is fully explained and states the
relationship with the child and adolescent. Informed consent is in duplicate, one of
which is kept by the parent or guardian and the other is kept by the researcher. During
the research process, if there is a major change in the research content, the revised
informed consent form should be sent to the ethics committee for approval. After the
approval, the researcher must fully communicate with the children and adolescents and
their parents or guardians, and confirm that they agree to continue to participate in
the study and re-sign the informed consent form.
6. Funds and insurance 6.1 funds Before the start of the study, funds were raised by the
Shanghai Eye Disease Prevention and Treatment Center.
