High Altitude Muscle Recovery — HAMR  

High Altitude Clinical Trial

Official title: Skeletal Muscle Recovery From Aerobic Exercise During Acute High Altitude Exposure When Consuming Carbohydrate Compared to Carbohydrate Plus Protein

Status Completed

Clinical Trial Summary

To understand alterations in glycogen and molecular regulation of skeletal muscle glucose uptake, glycogen synthesis, and muscle protein recovery when consuming CHO (glucose) or CHO+PRO (glucose + whey) post-exercise during unacclimatized high altitude exposure, randomized crossover double blinded studies will be conducted in the hypobaric/hypoxic chamber at USARIEM Table 1. Briefly, the study consists of a 2 day baseline period at SL followed by two, 3 day trial periods (with the 3rd day being a testing day) at HA. The baseline is separated from trial 1 for a least a day, and trial 1 & 2 separated by at least 4 days. Volunteers will consume CHO (glucose) or CHO+PRO (glucose + whey) drinks post-exercise during unacclimatized high altitude exposure during the two trial periods. The order of the drinks will be randomized (using a random number generator such as randomizer.org) and kept by a study staff not directly involved in data collection to maintain blinding.

Clinical Trial Description

Dietary strategies that restore muscle glycogen and promote muscle repair and remodeling in recovery from exercise are needed to protect muscle mass and sustain performance during strenuous military operations conducted at high altitude (HA; > 2500 m elevation). However, most military personnel who operate at HA are native lowlanders and, thus, unacclimatized to the hypoxemia that occurs upon initial HA exposure. We demonstrated that acute HA exposure (within 5 hours) dysregulates peripheral glucose uptake and inhibits post-exercise muscle anabolism in lowlanders sojourning at 4,300 m. Our results suggest that traditional recommendations to consume solely carbohydrate immediately post-exercise to facilitate muscle recovery may be inadequate for lowlanders exercising within the first several hours of HA exposure. In a recent meta-analysis by our group, we reported that the effects of ingesting carbohydrate (CHO) compared to isocaloric carbohydrate plus protein (CHO+PRO) on muscle glycogen repletion were equivocal, yet the combined macronutrient blend confers an additional metabolic advantage than CHO alone because it stimulates muscle repair/remodeling by upregulating anabolic signaling and protein synthesis. Our findings suggest post-exercise CHO intake can be reduced in place of an isocaloric amount of PRO when exercising at HA without comprising glycogen repletion. However, whether CHO+PRO promotes post-exercise glycogen repletion and muscle anabolism as compared to CHO alone when peripheral glucose uptake and anabolic signaling are dysregulated with acute HA exposure has not been determined. This study aims to determine the effects of ingesting CHO or an isocaloric amount of CHO+PRO post-aerobic exercise on glycogen synthesis, molecular regulation of glucose uptake, storage, and muscle protein synthesis during acute HA exposure. This study will employ a randomized crossover double-blinded design and be conducted in the hypobaric/hypoxic chamber at USARIEM. A total of 10 healthy, physically active men and women between the ages of 18-39 years will be studied. Following 48 hours of controlled exercise and diet to normalize muscle glycogen stores, volunteers will report to the laboratory after a 10-h overnight fast. After a muscle biopsy is taken from the vastus lateralis at baseline, volunteers will be brought to HA (460 mmHg) conditions within the hypobaric/hypoxic chamber at USARIEM and complete a standardized glycogen depletion protocol on a cycle ergometer. After completing the glycogen depletion exercise a second muscle biopsy will be taken. Volunteers will then consume 1.2 g CHO/kg/h or 0.9 g CHO/kg/h + 0.3 g PRO/kg/h over a 3-h recovery period. A third and fourth biopsy will be taken 4 and 6 hours into recovery to characterize the molecular regulation of glucose uptake, glycogen synthesis, and muscle protein synthesis over time. Serial blood draws will be collected during each trial to assess endocrine and circulating substrate responses in CHO and CHO+PRO. Trials will be separated by a minimum of 4 days. The primary risks associated with this study include those associated with acute hypobaric hypoxia, exercise, muscle biopsies, and blood draws. ;

Related Conditions & MeSH terms

• Altitude Sickness
• High Altitude

• NCT number: NCT06392373
• Study type: Interventional
• Source: United States Army Research Institute of Environmental Medicine
• Status: Completed
• Phase: N/A
• Start date: October 1, 2023
• Completion date: December 19, 2023