Effects of Exercise on Fructose Metabolism

Healthy Subjects Clinical Trial

— FruEx2  

Official title:

Effects of Exercise on Fructose Metabolism

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01866215
• Other study ID #: University of Lausanne
• Status: Completed
• Phase: N/A
• First received: May 27, 2013
• Last updated: June 3, 2014
• Start date: May 2013
• Est. completion date: January 2014

Study information

• Verified date: June 2014
• Source: University of Lausanne
• Contact: n/a
• Is FDA regulated: No
• Health authority: Switzerland: Ethikkommission
• Study type: Interventional

Clinical Trial Summary

A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate

1. how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals

2. how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 8
• Est. completion date: January 2014
• Est. primary completion date: January 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

• males

• 18-40 years old

• 19 kg/m2>BMI>25kg/m2

• moderate to high usual physical activity

• non-smokers

Exclusion Criteria:

• family history of diabetes mellitus

• ECG anomaly

• any medication

• participation to blood spending or other clinical study in the 3 months before the beginning of this study

• consumption of drugs

• consumption of more than 50g alcool/week

• family history of food intolerance

Study Design

Allocation: Randomized, Intervention Model: Crossover Assignment, Masking: Open Label, Primary Purpose: Basic Science

Related Conditions & MeSH terms

• Healthy Subjects

Intervention

Other:
• exercise
cycling at 100W during 60 min

Dietary Supplement:
• fructose
isocaloric nutrition with fructose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise
• glucose
isocaloric nutrition with glucose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise

Locations

Country	Name	City	State
Switzerland	Centre Hospitalier Universitaire Vaudois	Lausanne	Vaud

Sponsors (3)

Lead Sponsor	Collaborator
University of Lausanne	Centre Hospitalier Universitaire Vaudois, University of Bern

Country where clinical trial is conducted

Switzerland, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Plasma triglyceride	measurement of total and VLDL-TG concentrations in study 1	measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours)	No
Primary	intramyocellular lipids	Measurement by 1H-MRS in muscle vastus lateralis in study 2	after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise	No
Primary	Fructose conversion into lipids	Estimated from 13C palmitate-VLDL concentration	measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)	No
Secondary	Fructose conversion into glucose	measurements obtained from plasma 13C glucose concentrations and whole body glucose production measured by 6,6 d2 glucose in study 1)	measured between 7:00 am and 4:oo pm (up to 9 hours)	No
Secondary	intramyocellular glycogen concentrations	Measured by 1H-MRS in muscle vastus lateralis in study 2	after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise	No
Secondary	fructose oxidation	Calculated from breath 13CO2 production	measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)	No
Secondary	exogenous lipid oxidation	Lipids ingested will be labeled with 13C-triolein, and exogenous lipid oxidation will be measured from breath 13CO2 production	measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours)	No