Amino Acid Kinetics in Blood After Consuming Different Milk Protein Supplements

Healthy Participants Clinical Trial

Official title:

Does Native Whey Induce Greater Blood Leucine Concentrations Than Other Whey Protein Supplements and Milk: A Randomized Controlled Trial?

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02882386
• Other study ID #: NorwegianSSS
• Status: Completed
• Phase: N/A
• First received: August 16, 2016
• Last updated: August 24, 2016
• Start date: September 2012
• Est. completion date: February 2013

Study information

• Verified date: August 2016
• Source: Norwegian School of Sport Sciences
• Contact: n/a
• Is FDA regulated: No
• Health authority: Norway:National Committee for Medical and Health Research Ethics
• Study type: Interventional

Clinical Trial Summary

The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other milk protein supplements.

Description:

Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of leucine in native whey.

The aim of this double-blinded randomized 5-arm cross-over study is to compare amino acid kinetics in blood after a bout of strength training and ingestion of 20 grams of high quality, but distinct, dairy protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk). Furthermore, the investigators investigate whether differences in amino acid kinetics affect acute blood glucose and urea response, as well as recovery of muscle function after a bout of strength training.

The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other protein supplements.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 13
• Est. completion date: February 2013
• Est. primary completion date: October 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 45 Years

Eligibility

Inclusion Criteria:

• Healthy in the sense that they can conduct training and testing

• Able to understand Norwegian language written and oral

Exclusion Criteria:

• Diseases or injuries contraindicating participation

• Use of dietary supplements (e.g. proteins, vitamins and creatine)

• Lactose intolerance

• Allergy to milk

• Allergy towards local anesthetics (xylocain)

Study Design

Allocation: Randomized, Intervention Model: Crossover Assignment, Masking: Double Blind (Subject, Caregiver, Investigator, Outcomes Assessor), Primary Purpose: Basic Science

Related Conditions & MeSH terms

• Healthy Participants

Intervention

Other:
• Strength Training

Dietary Supplement:
• Milk 1%

• Whey protein concentrate 80 (WPC-80)

• Microparticulated whey

• Hydrolyzed whey

• Native whey

Locations

Country	Name	City	State
n/a

Sponsors (3)

Lead Sponsor	Collaborator
Norwegian School of Sport Sciences	Arkansas Children's Hospital Research Institute, Tine

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Plasma amino acid concentration change from baseline		Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements	No
Secondary	Serum glucose change from baseline		Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements	No
Secondary	Serum urea change from baseline		Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements	No
Secondary	Muscle force generating capacity change from baseline	Measured as unilateral isometric knee extension force (Nm) with 90° in the hip and knee joints.	Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey	No
Secondary	Jump height change from baseline	Measured as counter movement jump on a force plate	Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey	No
Secondary	Serum creatine kinase change from baseline		Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements	No
Secondary	Muscle soreness change from baseline	Measured for m. quadriceps and m. pectoralis major on a visual analog pain scale	Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey	No