The Influence of Liraglutide on the Reward Properties of Food: an fMRI Study on Healthy Volunteers

Healthy Human Male Subjects Clinical Trial

Official title:

The Influence of Liraglutide on the Reward Properties of Food: an fMRI Study on Healthy Volunteers

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT01695109
• Other study ID #: 702/2008
• Status: Completed
• Phase: Phase 4
• First received: September 19, 2012
• Last updated: September 26, 2012
• Start date: November 2010
• Est. completion date: March 2012

Study information

• Verified date: September 2012
• Source: Medical University of Vienna
• Contact: n/a
• Is FDA regulated: No
• Health authority: Austria: "Österreichische Agentur für Gesundheit und Ernährungssicherheit"
• Study type: Interventional

Clinical Trial Summary

Clinical experience has confirmed the anorexic effect of Glucagon-like-peptide 1 (GLP-1) mimetics in comparison to DPP-4 inhibitors. A possible mechanism of this effect might be associated with changes in food choices, as suggested by animal studies. It has been shown that functional magnetic resonance imaging (fMRI) of the brain is a valuable tool in obesity research and can be used to study the response of several brain regions to the visual presentation of preferred in comparison to non preferred food items and to non food items The aim of this study is to search for possible effects of liraglutide in comparison to placebo on 1. food choices and 2. changes in brain function as evidenced by fMRI in healthy volunteers.

Findings of this study will help not only to get deeper insight into the mechanism of the anorexic effect of GLP-1 mimetics, but also into the regulation of food choices per se. In the future, it is planned to extend the results of this study in normal weight volunteers to obese diabetic subjects.

Description:

Design: The study is designed as a double blind, placebo controlled, randomized crossover intervention study with two arms (liraglutide first followed by placebo vs. placebo first followed by liraglutide) in 16 healthy male volunteers. fMRI results and food intake are compared between groups baseline vs. end of treatment period and placebo vs. liraglutide.

Intervention: A standard dose of 0.6 mg Liraglutide (oder placebo) will be used, and will be applied subcutaneously each morning for three subsequent days by the study personnel. Study sessions will be performed on the fourth day after placebo/liraglutide interventions.

Study sessions: On study days participants arrive at the trial centre at 8.00 am after a 12h fast. Participants are instructed to eat a light dinner around 8.00 pm the previous day and to spend around 8h resting during the night before the study days. Upon arrival, blood pressure and heart rate is measured and an iv line is started, from which a blood sample is taken from the antecubital vein for blood glucose, insulin and plasma hormone concentrations determination. The occurrence of nausea, vomiting, dizziness or any other adverse events (AE's) is reviewed together with the subject.

Instructions for the fMRI examination are reviewed together with the subject. The subject is advised to leave all ferromagnetic items behind, and baseline hunger/satiety visual analogue scales (VAS) are recorded. Then, the fMRI session with the food items paradigm (together with repeated recordings of hunger/satiety VAS) takes place. Afterwards, another blood sample and hunger/satiety VAS is taken and the subject is allowed to eat ad libitum from a buffet breakfast. Food intake is recorded in detail, including meal choices and calorie intake. After a final hunger/satiety VAS recording and another blood sample subjects are dismissed from the trial centre.

Outcome variables: Main outcome variables are fMRI activity in the brain during the high calorie/low calorie food items paradigm and food intake at the buffet liraglutide vs. placebo at the end of treatment. Secondary outcome parameters are hunger/satiety VAS ratings, total and active plasma ghrelin, peptide YY (PYY), glucose and insulin concentrations.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 16
• Est. completion date: March 2012
• Est. primary completion date: February 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

• Male healthy volunteers

• Age 20-40

• Non-smokers

• Normal body weight (body mass index 19 -25 kg/m2)

• Right handed

• Signed informed consent

• Willingness and ability to comply with the protocol

Exclusion Criteria:

• Any history of or current drug abuse including alcohol consumption on a regular basis or binge drinking

• Any history of or current psychiatric disease, including any eating disorder, as assessed by structured interview

• Any history of dieting

• Any condition interfering with fMRI measurements such as ferromagnetic implants or claustrophobia

• Any evidence of relevant renal, liver, thyroid, cardiovascular, or respiratory illness at screening examination

• Any acute illness necessitating medical treatment during the last 3 weeks before study entry, any permanent intake of medication

• Any study participation in the last 3 months

• HIV, hepatitis B or C positive

• Any disease considered relevant for proper performance of the study or risks to the participant, at the discretion of the investigator

Study Design

Allocation: Randomized, Endpoint Classification: Efficacy Study, Intervention Model: Crossover Assignment, Masking: Single Blind (Subject), Primary Purpose: Basic Science

Related Conditions & MeSH terms

• Healthy Human Male Subjects

Intervention

Drug:
• Liraglutide
sc injections of 0.6 mg Liraglutide daily for three continuous days.
• Placebo
0.9% NaCl, 0.2 ml

Locations

Country	Name	City	State
Austria	Division of Endocrinology and Metabolism, Medical University of Vienna	Vienna

Sponsors (1)

Lead Sponsor	Collaborator
Medical University of Vienna

Country where clinical trial is conducted

Austria, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Food intake at an ad libitum buffet	Food intake at the ad libitum buffet under liraglutide vs. placebo will be measured in details: total amount of calories, and where these calories derive from	day 4	No
Primary	fMRI activity in the brain	fMRI activity in the brain during the high calorie/low calorie food items paradigm	day 4	No
Secondary	Changes in hunger and satiety scores on the visual analogue scales (VAS) over time as compared to baseline (measured in mm) during the study session	Hunger-VAS forms include the question: How hungry do you feel? Subjects are required to mark their feeling of hunger in a scale of 0 to 100 mm.; Satiety-VAS forms include the question: How satt do you feel? Subjects are required to mark their feeling of satiety in a scale of 0 to 100 mm.	timepoints -10, 90, 130 minutes	No
Secondary	Changes in Ghrelin, PYY, glucose and insulin concentrations during the study session	Blood samples will be withdrawn from an intravenous cannula placed circa 10 minutes before the start of the study in the left antecubital vein. Samples will be immediately cooled on ice, centrifuged at 3000 rpm for 10 minutes and then stored at -20°C for the later measurement of insulin, ghrelin and PYY. All assays will be performed using commercial assay kits at the very end of the study with samples belonging to (both study days) one subject being measured within one kit.	timepoints -10, 90, 130 minutes	No