Impact of Inulin on Production of Phenolic Acids From Tomato Onion and Lovage Soup

Healthy Adults Clinical Trial

Official title:

Impact of Inulin on Production of Phenolic Acids From Tomato Onion and Lovage Soup

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03577145
• Other study ID #: BB/MO27724/1-2
• Status: Completed
• Phase: N/A
• Start date: July 27, 2017
• Est. completion date: December 31, 2018

Study information

• Verified date: January 2019
• Source: University of Glasgow
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. The investigators hypothesize that combination of polyphenolics from a soup rich in rutin and quercitin and the non-digestible carbohydrate (NDC) inulin will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomized order: Tomato, onion and lovage soup (high polyphenol food), Inulin (NDC) or Mixture of tomato, onion and lovage soup and inulin.

During each feeding study, urine, blood and stool samples will be collected at regular intervals for the duration of 24 hrs after consumption of test food. Participants will be asked to follow a low polyphenol diet for 2 days prior to the feeding study.

Description:

Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols.

Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 15
• Est. completion date: December 31, 2018
• Est. primary completion date: December 31, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years to 70 Years

Eligibility

Inclusion Criteria:

• Self-reported healthy adults

Exclusion Criteria:

• Antibiotic use within the last 3 months

• Identified gastro-intestinal diseases

• On prescribed medication other than the contraceptive pill

• Pregnant or breastfeeding.

• Diagnosed as anaemic

• Allergic to paracetamol or any food

Related Conditions & MeSH terms

• Healthy Adults

Intervention

Dietary Supplement:
• Tomato, onion & lovage soup with inulin
Source of polyphenols and non digestible carbohydrate
• Tomato, onion & lovage soup
Source of polyphenols
• Inulin
Source of non digestible carbohydrate

Locations

Country	Name	City	State
United Kingdom	School of Medicine, Nursing and Dentistry, College of MVLS, University of Glasgow	Glasgow	Lanarkshire

Sponsors (2)

Lead Sponsor	Collaborator
University of Glasgow	Scottish Universities Environmental Research Centre

Country where clinical trial is conducted

United Kingdom, 

References & Publications (3)

Roowi S, Mullen W, Edwards CA, Crozier A. Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans. Mol Nutr Food Res. 2009 May;53 Suppl 1:S68-75. doi: 10.1002/mnfr.200800287. — View Citation

Russell W, Duthie G. Plant secondary metabolites and gut health: the case for phenolic acids. Proc Nutr Soc. 2011 Aug;70(3):389-96. doi: 10.1017/S0029665111000152. — View Citation

Tamura M, Nakagawa H, Tsushida T, Hirayama K, Itoh K. Effect of pectin enhancement on plasma quercetin and fecal flora in rutin-supplemented mice. J Food Sci. 2007 Nov;72(9):S648-51. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Phenolic acids bioavailability	Urine excretion of phenolic acids (µg) will be measured with GC-MS	0-24 hrs
Secondary	Glycaemic measurements	Plasma glucose (mmol/L), insulin (mU/L) will be measured by commercial kits	0-8 hrs
Secondary	Appetite hormones measurements	Appetite hormone (PYY) levels in plasma (pg/mL) will be measured by commercial Elisa kit	0-8 hrs
Secondary	Mouth to caecum transit time	Mouth to caecum transit time (in hours/mins) will be calculated from sustained rise in breath hydrogen level measurements by hydrogen monitor	0-8 hrs
Secondary	Gastric emptying time	Gastric emptying time (in hours/mins) will be estimated using kinetics of plasma paracetamol levels measured by acetaminophen assay kits	0-6 hrs