Vaccination With Lethally Irradiated Glioma Cells Mixed With GM-K562 Cells in Patients Undergoing Craniotomy For Recurrent Tumor

Glioma Clinical Trial

Official title:

A Phase I Study of Vaccination With Lethally Irradiated Glioma Cells Mixed With GM-K562 Cells in Patients Undergoing Craniotomy For Recurrent Tumor

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT00694330
• Other study ID #: 06-349
• Status: Completed
• Phase: Phase 1
• First received: June 6, 2008
• Last updated: June 20, 2017
• Start date: June 2008
• Est. completion date: August 2009

Study information

• Verified date: March 2017
• Source: Massachusetts General Hospital
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This research study is testing the safety of a vaccination of cells called GM-K562 cells mixed with the participants own irradiated tumor cells. The GM-K562 cells have been modified in the laboratory to secrete the protein GM-CSF. This protein can be effective in stimulating an immune response to cancer. This newly developed vaccine may stop cancer cells from growing.

Description:

• Participants will undergo a craniotomy (brain surgery) to remove as much of the tumor as possible from their brain. A pathologist will examine the tissue to determine whether the cells are viable. If not, we will not be able to make the vaccine and the participant will not be eligible to proceed with this treatment protocol.

• The dose of the vaccine will be determined by the number of tumor cells that we are able to collect from the surgery. We will also be trying to determine the appropriate number of GM-K562 cells that can be given safely. We will do this by assigning groups of participants to different dose levels of GM-K562.

• For the first three weeks of this study, vaccines will be given once each week. After the first three weeks, vaccines will be given every other week.

• Before the first and after the fourth vaccinations, a small amount of the participants own irradiated tumor cells will be injected under their skin to see if their immune system will react against it. If the participant develops a rash, they may be asked to undergo a small skin biopsy for additional evaluation. These biopsies are optional.

• During the course of the study, we will also be collecting blood samples to evaluate the effect that the vaccinations may have on the immune system. These tests will be done every month.

• Participants will have an MRI once every two months.

• The following tests and procedures will be done before the participant receive the vaccine and every two weeks (During the first month, these will be performed on Days 1, 3 or 4, 15, 29 and 31 or 32): medical history; physical exam; vital signs; neurological exam; routine blood tests; research blood tests.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 11
• Est. completion date: August 2009
• Est. primary completion date: August 2009
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Patients must have recurrent malignant glioma, having already been diagnosed with and treated for biopsy-proven glioblastoma, anaplastic astrocytoma, anaplastic oligodendroglioma, anaplastic mixed oligoastrocytoma or gliosarcoma.

• Patients will already have been treated with external beam irradiation with or without chemotherapy.

• Patients must be able to undergo a MRI.

• A priori clinical indication for open resection/debulking of recurrent malignant glioma.

• At the time of vaccination, patients must be at least 4 weeks from completion of radiotherapy and 4 weeks from cytotoxic chemotherapy.

• Serum absolute neutrophil count greater than or equal to 1500/mm3

• Serum platelets greater than or equal to 50,000/mm3

• Serum sodium greater than or equal to 125 mmol/L

• 18 years of age or older

• Karnofsky Performance Score of 60% or greater

Exclusion Criteria:

• Uncontrolled active infection

• Pregnancy or nursing mothers

• HIV infection

• Evidence of active infection with Hepatitis B or C

• Concurrent malignancy

• Active or clinically relevant autoimmune disease

• Urgent need for surgical decompression (at the time of initial consultation)

• Previous participation in a gene therapy or immunotherapy trial

• Inability to provide informed consent because of altered mental status or mental illness

• Any other medical, surgical or psychiatric condition which could interfere with the patient's inability to comply with protocol regimen

Related Conditions & MeSH terms

• Glioma

Intervention

Biological:
• GM-K562 Vaccination
Lethally irradiated autologous glioma cells combined with GM-K562 cells as vaccine therapy given once a week for three weeks then every other week.

Locations

Country	Name	City	State
United States	Dana-Farber Cancer Institute	Boston	Massachusetts
United States	Massachusetts General Hospital	Boston	Massachusetts

Sponsors (3)

Lead Sponsor	Collaborator
Massachusetts General Hospital	Brigham and Women's Hospital, Dana-Farber Cancer Institute

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	To determine the feasibility of preparing lethally irradiated autologous glioma cells for use in combination with irradiated GM-K562 cells as vaccination therapy in this patient population.		3 years
Primary	To determine the safety and biological activity of subcutaneous and intradermal injection of irradiated autologous malignant glioma cells mixed with irradiated GM-CSF producing GM-K562 cells in this patient population.		3 years
Secondary	Progression free survival		3 years
Secondary	overall survival		5 years