Genodermatosis Clinical Trial
Official title:
Development of a Genetic Analysis Method by Mendeliomes and Genomes in the Diagnosis of Genodermatoses and Rare Genetic Diseases With Cutaneous Expression
The goal of the study is to develop a method of genetic diagnosis in two stages, by mendelioma then by genome and transcriptome on fibroblast culture, in genodermatoses and rare diseases with cutaneous expression in the child.
Interventional multicenter prospective study. Patients will be examined by a dermatologist to
describe and identify the various skin lesions Collaboration with the geneticist team:
clinical examination for relevant cases Patient records will be consulted. Relevant medical
information, biological examinations and other complementary examinations will be studied.
A blood sample (10 ml in EDTA tube) will be collected from the patient and his/her parents to
store DNA for mediome and genome.
A written parental and child consent (if age-appropriate) will be obtained and a study
information sheet will be signed. They will also sign the usual genetic consent request for
mendeliome, genome and transcriptome on culture of fibroblasts.
A 4 mm punch skin biopsy (healthy or damaged depending on phenotype and indication) will be
performed according to the standard technique.
The fibroblast culture will be performed routinely by the Genetics Center Transcriptome will
be done according to the processes set up at the Genetics Center Mendeliome analysis
- Allow the analysis of 4000 rare disease genes
- Will be performed according to routine analyzes of the genetics lab
- Uses the Highlander tools (web)
- Use of de novo filters, autosomal recessive, heterozygous compound, X linked, strong
variant (LOF and canonical splice sites)
- Use of rarer filters: exomic or gene deletion, splicing (+/- 12 base pairs around exons)
- In unexplained severe cases, a genome supplemented with the 10Xgenomics method and a
transcriptome of fibroblasts will be realized. This double strategy afford to get a
genome of high interpretative quality. Genome analysis by the 10Xgenomics method
(https://www.10xgenomics.com )
- This method allows us to deconvolate haplotypes and allows the analysis of 16,000 other
complementary genes and to obtain precisely defined structural variants.
- The transcriptome will better assess the genomic effects on gene expression.
- The genome and transcriptome will also assess the presence of deep intron mutations and
their effect on splicing, and will be a sustainable resource for other long-term
projects (analysis of non-coding regions, microRNAs, etc.)
;
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Active, not recruiting |
NCT00001813 -
Examination of Clinical and Laboratory Abnormalities in Patients With Defective DNA Repair: Xeroderma Pigmentosum, Cockayne Syndrome, or Trichothiodystrophy
|