Exercise-induced Changes in Exosomes

Exercise Clinical Trial

Official title:

Exercise-induced Changes in Exosomes

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05616234
• Other study ID #: 1956959
• Status: Completed
• Phase: N/A
• Start date: November 7, 2022
• Est. completion date: February 17, 2023

Study information

• Verified date: June 2023
• Source: University of California, Davis
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 12
• Est. completion date: February 17, 2023
• Est. primary completion date: February 17, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Young healthy adults (18-35 y)

• Experience with exercise (>1y)

Exclusion Criteria:

• Pregnancy

• Smoking

• Receiving any medication that may interfere with the study outcomes

Related Conditions & MeSH terms

• Connective Tissue
• Exercise
• Exosomes

Intervention

Procedure:
• Resistance Exercise
Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Locations

Country	Name	City	State
United States	Hickey Laboratory	Davis	California

Sponsors (1)

Lead Sponsor	Collaborator
University of California, Davis

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Collagen content of ligament constructs	Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.	15 min post-exercise
Secondary	Ultimate tensile stress and modulus of ligament constructs	ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus.	15 min post-exercise
Secondary	Exosomal RNA	Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise.	15 min post-exercise