Influence of Endurance Exercise and Histamine Receptors on the Gene Expression in Skeletal Muscle

Exercise Clinical Trial

Official title:

Influence of Endurance Training and Histamine Receptor Antagonists on the Transcriptome Response in Human Muscle

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05131555
• Other study ID #: AET - BC-10237
• Status: Completed
• Phase: N/A
• Start date: August 16, 2021
• Est. completion date: December 31, 2021

Study information

• Verified date: April 2024
• Source: University Ghent
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 14
• Est. completion date: December 31, 2021
• Est. primary completion date: December 6, 2021
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 45 Years

Eligibility

Inclusion Criteria:

• Male and female

• 18-45 years

• not to medium physically active

Exclusion Criteria:

• Smoking

• Chronic disease

• Supplement or medication intake

• Seasonal allergies

Related Conditions & MeSH terms

• Exercise
• Histamine

Intervention

Drug:
• Placebo: Placebo + exercise training.
Oral placebo Exercise training: Interval-based cycling exercise
• H1 blockade: H1 receptor antagonist + exercise training
H1 blockade: oral blockade Exercise training: Interval-based cycling exercise
• H2 Blockade: H2 receptor antagonist + exercise training
H2 blockade: oral blockade Exercise training: Interval-based cycling exercise

Locations

Country	Name	City	State
Belgium	Department of movement and sports sciences, Ghent University, Belgium	Ghent	Oost-Vlaanderen

Sponsors (2)

Lead Sponsor	Collaborator
University Ghent	Research Foundation Flanders

Country where clinical trial is conducted

Belgium, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in muscle transcriptome (direct response)	RNA-sequencing	On each test-day: 0 minutes after the exercise training versus at rest.
Primary	Change in muscle transcriptome (delayed response)	RNA-sequencing	On each test-day: 180 minutes after the exercise training versus at rest.
Primary	Muscle glycogen depletion	Fluorometric determination of muscle glycogen levels	On each test-day: 0 minutes after the exercise training versus at rest.
Primary	Muscle glycogen resynthesis	Fluorometric determination of muscle glycogen levels	On each test-day: 180 minutes after the exercise training versus at rest.
Primary	Plasma volume change	Plasma volume based on hemoglobin and hematocrit concentration.	Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).
Secondary	Heart rate during the exercise training	Heart rate during the exercise training.	Continuously during training on each test-day.
Secondary	Blood lactate	Capillary lactate concentration.	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Secondary	Blood glucose	Capillary glucose concentration.	On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
Secondary	Blood histamine	histamine concentration in blood samples.	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Secondary	Blood insulin	insulin concentration in blood samples.	On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
Secondary	Muscle signaling pathways relevant for glucose metabolism and cardiovascular health	Phosphorylation status of proteins assessed by Western Blotting	On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.