Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men

Exercise Clinical Trial

— PECS  

Official title:

The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04596553
• Other study ID #: H20-00943
• Status: Completed
• Phase: N/A
• Start date: January 25, 2021
• Est. completion date: February 13, 2022

Study information

• Verified date: June 2023
• Source: University of British Columbia
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Description:

This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 17
• Est. completion date: February 13, 2022
• Est. primary completion date: February 13, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

• Can fluently read and write in English
• Male
• 18 - 35 years

Exclusion Criteria:

• Diabetes, cardiovascular disease, or current pharmaceutical therapy
• Medical conditions impacting ability to undertake strenuous physical activity
• Lower body resistance training in the previous 6 months
• BMI > 30
• Currently smoking with cigarettes
• Allergy to any study supplements
• Currently taking any protein, amino acid, or collagen supplement
• Participate in greater than 2 hours of weekly vigorous exercise

Related Conditions & MeSH terms

• Amino Acids Metabolism
• Dietary Supplements
• Exercise
• Extracellular Matrix
• Skeletal Muscle

Intervention

Dietary Supplement:
• Essential Amino Acid
Immediately following the exercise intervention participants will consume their assigned essential amino acid supplement
• Collagen Peptide
Immediately following the exercise intervention participants will consume their assigned collagen peptide supplement
• Maltodextrin Placebo
Immediately following the exercise intervention participants will consume their assigned maltodextrin supplement

Locations

Country	Name	City	State
Canada	Chan Gunn Pavilion	Vancouver	British Columbia

Sponsors (1)

Lead Sponsor	Collaborator
University of British Columbia

Country where clinical trial is conducted

Canada, 

References & Publications (7)

Alcock RD, Shaw GC, Tee N, Burke LM. Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males. Front Nutr. 2019 Oct 15;6:163. doi: 10.3389/fnut.2019.00163. eCollection 2019. — View Citation

Egerman MA, Glass DJ. Signaling pathways controlling skeletal muscle mass. Crit Rev Biochem Mol Biol. 2014 Jan-Feb;49(1):59-68. doi: 10.3109/10409238.2013.857291. Epub 2013 Nov 18. — View Citation

Frontera WR, Ochala J. Skeletal muscle: a brief review of structure and function. Calcif Tissue Int. 2015 Mar;96(3):183-95. doi: 10.1007/s00223-014-9915-y. Epub 2014 Oct 8. — View Citation

Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve. 2017 Jan;55(1):128-130. doi: 10.1002/mus.25398. Epub 2016 Sep 26. — View Citation

Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, Kjaer M. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E257-69. doi: 10.1152/ajpendo.00609.2009. Epub 2009 Nov 10. — View Citation

Miller BF, Olesen JL, Hansen M, Dossing S, Crameri RM, Welling RJ, Langberg H, Flyvbjerg A, Kjaer M, Babraj JA, Smith K, Rennie MJ. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol. 2005 Sep 15;567(Pt 3):1021-33. doi: 10.1113/jphysiol.2005.093690. Epub 2005 Jul 7. — View Citation

Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017 Jan;105(1):136-143. doi: 10.3945/ajcn.116.138594. Epub 2016 Nov 16. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis	Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.	0 - 4 hours post-exercise
Primary	Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis	Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.	0 - 2 hours post-exercise
Primary	Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis	Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.	2 - 4 hours post-exercise
Primary	Fractional synthetic rate (change over time) Muscle Collagen Synthesis	Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.	0 - 4 hours post-exercise
Primary	Fractional synthetic rate (change over time) Muscle Collagen Synthesis	Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.	0 - 2 hours post-exercise
Primary	Fractional synthetic rate (change over time) Muscle Collagen Synthesis	Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.	2 - 4 hours post-exercise
Primary	Pro-Collagen Type I N-Propeptide (PINP)	Osteoblastic marker of bone collagen synthesis	1 hour prior to exercise
Primary	Pro-Collagen Type I N-Propeptide (PINP)	Osteoblastic marker of bone collagen synthesis	4 hours post exercise
Secondary	Plasma Amino Acids Response to Supplementation	Amino acid plasma concentrations	0 - 4 hours post-exercise
Secondary	Muscle mechanistic target of rapamycin Pathway Activation	Measuring the fold change in enzymes associated with the anabolic signalling of the mTOR pathway (e.g. phosphoinositide 3-kinases (PI3k), protein kinase B (Akt))	0 - 4 hours post-exercise
Secondary	PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)	Measuring the fold change when the collagen genes of interest become detectable in the PCR analysis	0 - 4 hours post-exercise
Secondary	Pro-Collagen Type III N-Propeptide (PIIINP)	Marker of collagen synthesis	1 hour prior to exercise
Secondary	Pro-Collagen Type III N-Propeptide (PIIINP)	Marker of collagen synthesis	4 hours post exercise