Muscle Response to High Intensity Interval Resistance Training (HIIRT)

Exercise Clinical Trial

— HIIRT  

Official title:

Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04321772
• Other study ID #: HIIRT12
• Status: Completed
• Phase: N/A
• Start date: February 1, 2012
• Est. completion date: February 10, 2013

Study information

• Verified date: March 2020
• Source: University of Padova
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Description:

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Recruitment information / eligibility

• Status: Completed
• Enrollment: 9
• Est. completion date: February 10, 2013
• Est. primary completion date: May 15, 2012
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years to 30 Years

Eligibility

Inclusion Criteria:

• moderate active

• experience with resistance training

Exclusion Criteria:

• history of recent myocardial infarction,

• severe cardiac arrhythmia,

• unstable angina,

• poorly controlled hypertension,

• poorly controlled diabetes mellitus,

• frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Related Conditions & MeSH terms

• Exercise
• Resistance Training

Intervention

Other:
• High Intensity Interval Resistance Training
Subjects trained one leg with HIIRT
• Traditional Resistance Training
Subjects trained the other leg with HIIRT

Locations

Country	Name	City	State
Italy	Nutrition and Exercise Lab, DSB, University of Padova	Padova

Sponsors (2)

Lead Sponsor	Collaborator
University of Padova	University of Roma La Sapienza

Country where clinical trial is conducted

Italy, 

References & Publications (3)

Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237. — View Citation

Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22. — View Citation

Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	pAMPKThr172 in skeletal muscle tissue measured by immunoblot	measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy	24 hours
Primary	pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot	measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy	24 hours
Primary	pS6Ser235/236 in skeletal muscle tissue measured by immunoblot	measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy	24 hours
Primary	p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot	measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy	24 hours
Primary	ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot	ACC phosphorylation in skeletal muscle tissue taken by biopsy	24 hours
Primary	IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IGF-1 mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IGF-1ea mRNA in skeletal muscle tissue taken by biopsy	24 hoursn
Primary	MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	MGF mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	STARS mRNA in skeletal muscle tissue taken by biopsy	24 hoursn
Primary	Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Myogenin mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	IL-6 mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	PGC1a4 mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	PGC1a mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Atrogin mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Beclin mRNA in skeletal muscle tissue taken by biopsy	24 hours
Primary	Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)	Myostatin mRNA in skeletal muscle tissue taken by biopsy	24 hours