The Impact of Acute (2 h) and Short-term (4 Days) Nitrate Supplementation in the Form of Red Beet Root Juice on Intense Intermittent Exercise Performance.

Exercise Performance Clinical Trial

Official title:

The Impact of Acute (2 h) and Short-term (4 Days) Nitrate Supplementation in the Form of Red Beet Root Juice on Intense Intermittent Exercise Performance.

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03616093
• Other study ID #: PEP-1718
• Status: Completed
• Phase: N/A
• Start date: September 30, 2017
• Est. completion date: May 31, 2018

Study information

• Verified date: August 2018
• Source: PepsiCo Global R&D
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Both acute ingestion (1-3 h) and chronic supplementation (3-30 days) with dietary nitrate has been shown to increase nitric oxide activity and, in some studies, to improve exercise economy, exercise tolerance, and endurance exercise performance. In addition, more recent evidence suggests that dietary nitrate has the potential to enhance team-sport-specific high-intensity intermittent exercise performance. Indeed, chronic dietary nitrate supplementation (2-6 days) has been reported to increase the distance covered before exhaustion during the Yo-Yo intermittent recovery level 1 test, a well-established and ecologically valid test widely used to mimic the high-intensity running bouts of soccer match-play. However, while these findings suggest that nitrate may be an effective ergogenic aid for team-sport players when consumed daily, it is currently unclear if the improvement in team-sport specific intermittent exercise performance can be achieved following a single bolus of dietary nitrate. Given the increasing interest and use of dietary nitrate as an ergogenic aid by team-sport players, establishing the shortest period of supplementation required to elicit an ergogenic effect is important in order to guide athletes on optimal supplementation strategies.

Given that a single bolus of dietary nitrate has previously been shown to improve exercise tolerance and endurance exercise performance, and an improved Yo-Yo IR1 performance has been observed after chronic nitrate supplementation, the investigator hypothesizes that acute (2 h) and short-term (4 days) nitrate supplementation will significantly elevate nitric oxide activity (measured as an increase in plasma nitrite concentration and skeletal muscle nitrate concentration) and improve performance during the Yo-Yo IR1 test (measured as an increased in distance covered (m) during the test at the point of volitional exhaustion) to a similar extent, when compared to a placebo.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 16
• Est. completion date: May 31, 2018
• Est. primary completion date: May 1, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 35 Years

Eligibility

Inclusion Criteria:

1. Male, physically active, intermittent team sport players, in self-reported good general health as assessed by the standard procedures described below and specifically meeting the following: blood pressure (BP), systolic 100-135 mmHg and diastolic 60-90 mmHg, and; body mass index (BMI) 16-29.9.

2. 18-35 years of age

3. Participants must participate in an intermittent exercise based sport (e.g. soccer, rugby, hockey), at a competitive level (i.e. competes in officiated matches/games).

4. Understanding of the procedures to be undertaken as part of the study

5. Willingness to participate in exercise testing and follow supplementation guidelines and other instructions provided by the experimenter

6. Informed, voluntary, written consent to participate in the study

Exclusion Criteria:

1. Known pulmonary, cardiovascular or metabolic disease

2. Food allergies including phenylketonurea (PKU)

3. Current regular use of any dietary supplement (excluding macronutrient based supplements) or previous use of any dietary supplement in the past 6 months that is known to have a lasting effect.

4. Blood donation within 3 months prior to the start of the study

5. Substance abuse within 2 years of the start of the study

6. Smoking

7. Any prescription and non-prescription medication which may impact saliva production (antidepressants, diuretics, analgesics, antihistamines, anti-hyper/hypo-tensives, anti-anxiety, appetite suppressants).

8. Current or previous use of phosphodiesterase 5 (PDE5) inhibitors.

9. Participation in another clinical trial within past 4 weeks and/or participation in a PepsiCo trial within the last 6 months.

10. Chronic use (6 months) of any antibacterial mouthwash products.

Related Conditions & MeSH terms

• Exercise Performance

Intervention

Other:
• Test Beet Shot
Active supplement containing 6.2 mmol nitrate
• Placebo Beverage
Placebo supplement containing negligible nitrate

Locations

Country	Name	City	State
United Kingdom	University of Exeter, Sport and Health Sciences department,	Exeter

Sponsors (1)

Lead Sponsor	Collaborator
PepsiCo Global R&D

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Distance covered during high-intensity intermittent exercise performance		4 days
Primary	Distance covered during high-intensity intermittent exercise performance		2 hours
Secondary	Plasma nitrate concentrations		4 days
Secondary	Nitrite concentrations		2 hours