Study of Anti-Mesothelin Car NK Cells in Epithelial Ovarian Cancer

Epithelial Ovarian Cancer Clinical Trial

Official title:

Clinical Study on the Safety and Efficacy of Anti-Mesothelin Car NK Cells With Epithelial Ovarian Cancer

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT03692637
• Other study ID #: Mesothelin Car NK-HNRM-01
• Status: Not yet recruiting
• Phase: Early Phase 1
• Start date: March 2019
• Est. completion date: November 2021

Study information

• Verified date: September 2018
• Source: Allife Medical Science and Technology Co., Ltd.
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is a single centre、single arm、open-label，to investigate the safety and efficacy of anti-Mesothelin Car NK Cells With Epithelial ovarian cancer

Description:

Ovarian cancer ranks second in gynecologic malignancies, but mortality ranks first. It is one of the most serious malignant tumors that threaten women's lives. It is urgent to explore new treatment methods, such as introduction. Tumor immunotherapy strategies to improve treatment outcomes and patient quality of life. Car is a group of fusion proteins composed of multiple parts, including antigen recognition region, transmembrane region, and NK cell stimulation region. NK cells are naive immune cells that are not restricted by MHI and can be activated without additional co-stimulatory regions. After activation, granulin-B and γ-interferon are released, thereby inducing apoptosis of tumor cells and lysis of tumor cells. The study will follow a 3 + 3 design of dose- escalating cohorts. After a patient enrolls, leukapheresis will be performed to obtain peripheral blood mononuclearcells which will be sent to a manufacturing site to produce anti-Mesothelin Car NK cells. The cells will then be returned to the investigational site and, after a standard chemotherapy based conditioning regimen, will be administered to the patient. Treated patients will undergo serial measurements of safety, tolerability and response.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 30
• Est. completion date: November 2021
• Est. primary completion date: November 2020
• Accepts healthy volunteers: No
• Gender: Female
• Age group: 18 Years to 70 Years

Eligibility

Inclusion Criteria:

1. Mesothelin-positive patients with stage II-IV epithelial ovarian cancer.(Mesothelin expression =50% of tumor cells)(Patients with recurrence are not excluded)

2. Male or female, 18 to 70 years old (including 18 and 70 years old)

3. The excepted survival = 6 months,Karnofsky activity function status score = 60 points

4. there are measurable lesions according to the RECIST 1.1 standard.

5. Bone marrow function (blood routine) satisfies: the number of neutrophils > 1×109/L platelet > 7.5×109/L; hemoglobin > 90 g/L; total bilirubin < 2.0 mg/dL; creatinine < 1.5 times the upper limit; albumin < 2 g/L; ALT or AST < 5 times the upper limit; creatinine clearance rateby Cockcroft-Gault formula< 40 mL/min

6. Coagulation parameters: INR = 1.5, PTT < 1.2 times the upper limt

7. Patients did not receive any chemotherapy, radiotherapy, immunotherapy (such as immunosuppressive drugs PD-1, PDL-1) or other anticancer treatment 2-4 weeks before admission, and the treatment-related toxicity reaction =1 level prior to enrollment(except low toxicity lose hair for example)

8. Venous channel is unobstructed, which can meet the needs of intravenous drip

9. HIV test was negative; HBV and HCV were negative

10. Voluntary informed consent is given, agree to follow the trial treatment and visit plan

Exclusion Criteria:

1. Patients with hypertension (> 160/95 mmHg) are uncontrollable; coronary heart disease and angina pectoris are unstable (Canadian Cardiovascular Association Level II and above), or recurrent within 6 months

2. Patients suffer from concurrent or central nervous system diseases

3. Patients with severe autoimmune diseases or immunodeficiency diseases

4. Patient's lung function has the following abnormalities: FEV (forced expiratory volume), < 30%; DLCO (diffusing capacity of lung for carbon monoxide) < 30%; oxygen saturation < 90%

5. Patients treated with other immune cellular products (DC, CIK, T, NK, and Car T products with CD19 or other targets)

6. Patients with Severe liver and kidney dysfunction

7. Patients with Severe bacterial and viral infections

8. Patients are pregnant or nursing

9. Patients used corticosteroids within 4 weeks prior to enrollment(except patients with inhaled hormone)

10. Patients participated in other clinical trials within 30 days.

11. Patients had other conditions that were not appropriate for the group determined by the researchers.

Related Conditions & MeSH terms

• Epithelial Ovarian Cancer
• Neoplasms, Glandular and Epithelial
• Ovarian Neoplasms

Intervention

Biological:
• anti-Mesothelin Car NK Cells
anti-Mesothelin Car NK Cells injection

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Allife Medical Science and Technology Co., Ltd.

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Occurrence of treatment related adverse events as assessed by CTCAE v4.0	defined as >= Grade 3 signs/symptoms, laboratory toxicities, and clinical events that are possibly, likely, or definitely related to study treatment	Day 3-Year 2 after injection