Detection of Enterovirus Genotypes by CRISPR Technology

Status Withdrawn

Clinical Trial Summary

CRISPR-enterovirus detection system was constructed in this study for detection variety genotypes of enterovirus rapidly in children suspected or diagnosed as enterovirus infection.

Clinical Trial Description

To construct CRISPR-enterovirus detection system, we screened the targets, primers and CrRNA and evaluated the capability of this detecting system. Samples of feces, blood and cerebrospinal fluid (the remainder of routine testing samples) from 500 children with suspected or confirmed enterovirus infection were examined using the CRISPR technique. To evaluate the CRISPR-enterovirus detection system, real-time PCR and traditional PCR as comparison method was implemented. The method of real-time PCR and traditional PCR were used to detect enterovirus and analyze the genotypes of enterovirus, respectively. According to the results of CRISPR-enterovirus detection system and the comparison method, the positive coincidence rate, negative coincidence rate and consistency of the two methods were calculated. Furthermore, the sensitivity and specificity of the CRISPR-enterovirus detection system for detection of enterovirus were calculated. ;

Study Design

Related Conditions & MeSH terms

Enterovirus Infections

Clinical Trial Details

NCT number	NCT04535648
Study type	Observational
Source	Children's Hospital of Fudan University
Contact
Status	Withdrawn
Phase
Start date	April 2023
Completion date	September 2024

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