Interrupting Sitting With High Intensity Exercise on Vascular Function in Adolescents Before and After a Mixed Meal

Endothelial Dysfunction Clinical Trial

Official title:

The Effect of Prolonged vs. Interrupted Sitting With High Intensity Interval Exercise on Lower Limb Vascular Function in Adolescents Before and After a Mixed Meal

Clinical Trial Details — Status: Terminated

Administrative data

• NCT number: NCT04337385
• Other study ID #: 191204/B/04
• Status: Terminated
• Phase: N/A
• Start date: March 4, 2020
• Est. completion date: March 13, 2020

Study information

• Verified date: May 2021
• Source: University of Exeter
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study aims to investigate the effect of interrupting a three hour bout of prolonged sitting with high intensity interval exercise on lower limb blood vessel function in adolescents before and after a mixed meal challenge.

Description:

Participants will be required to visit the laboratory on three separate occasions. The first of which will be a preliminary visit to collect anthropometric data (e.g. stature and body weight) and participants will complete a combined ramp-incremental and supra-maximal test to exhaustion on a cycle ergometer to determine the work rate for the exercise in the subsequent experimental visits.

For the two experimental visits, participants will arrive to the laboratory after an overnight fast and an activPAL accelerometer will be attached to the midline of the participants' thigh for the duration of the visit to quantity sitting time.

Lower limb macro and micro-vascular function will be assessed using a single protocol through flow mediated dilation of the popliteal artery and post-occlusive reactive hyperaemia in the capillaries of the calf. Participants will then either sit for three hours or have this three hour sitting period interrupted with high intensity interval cycling exercise at 30, 90 and 150 minutes. Vascular function will then be reassessed before participants will be given a mixed meal challenge (58.4 g fat and 53.4 g sugar) with a three hour postprandial period where the participants will remain seated for the duration. At the end of the postprandial period, vascular function will be reassessed.

Blood pressure, heart rate and calf circumference will be assessed hourly throughout the experimental day. In addition, capillary blood samples will be collected at baseline, pre meal and each hour during the postprandial period and assessed for changes in plasma glucose, triglyceride, cholesterol and insulin concentrations.

Participants will be transported to the toilet by wheelchair to limit the amount of walking undertaken during the sitting period and this activity will be quantified by the activPAL accelerometers.

Recruitment information / eligibility

• Status: Terminated
• Enrollment: 6
• Est. completion date: March 13, 2020
• Est. primary completion date: March 13, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 12 Years to 15 Years

Eligibility

Inclusion Criteria:

• Male and female adolescents

• 12-15 years

Exclusion Criteria:

• Medications known to affect blood pressure, carbohydrate or fat metabolism

• Allergies to the test meal e.g. dairy or gluten

• Contraindications to exercise

• Individuals with special educational needs that mean they are unable to fully comprehend the demands of the study procedures

Related Conditions & MeSH terms

• Cardiovascular Risk Factor
• Endothelial Dysfunction

Intervention

Behavioral:
• Interrupting 3 hours of prolonged sitting with high intensity interval exercise
Breaking up a prolonged bout of sitting in adolescents using high intensity interval exercise
• 3 hours of prolonged sitting
Participants will be asked to remain seated for 3 hours and refrain from moving their lower limbs

Locations

Country	Name	City	State
United Kingdom	University of Exeter	Exeter	Devon

Sponsors (2)

Lead Sponsor	Collaborator
University of Exeter	University of British Columbia

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Other	Participant Enjoyment During the High Intensity Interval Exercise	The enjoyment of the high intensity interval exercise will be assessed during the exercise bouts using the Exercise Enjoyment Scale (ESS) (Stanley & Cumming, 2010). The scale runs from 1 (not at all) to 7 (extremely) so a larger score indicates greater enjoyment.	3 hours
Other	Participant Enjoyment Post the High Intensity Interval Exercise	Participants will rate their enjoyment post exercise using the Physical Activity Enjoyment Scale (PACES) modified for adolescents (Motl et al., 2001). Participants rate how much they agree (1 = "disagree a lot" to 5 = "agree a lot") with 16 statements about their feelings from the exercise. The combined enjoyment scale score can range from 16 to 80 with a higher score indicating greater perceived enjoyment.	3 hours
Primary	Popliteal macrovascular function	Flow mediated dilation will be used to assess changes in microvascular function of the popliteal artery	Before and after 3 hours of prolonged vs. interrupted sitting and after a subsequent 3 hour postprandial period
Secondary	Microvascular function in the capillaries of the calf	Microvascular function will be assessed using post occlusive reactive hyperaemia via laser Doppler flowmetry	Before and after 3 hours of prolonged vs. interrupted sitting and after a subsequent 3 hour postprandial period
Secondary	Changes in plasma triglyceride concentration from a capillary sample	Changes in plasma triglyceride concentration will be assessed in response to the prolonged or interrupted sitting and after mixed meal	6.5 hours
Secondary	Changes in plasma insulin concentration from a capillary sample	Changes in plasma insulin concentration will be assessed in response to the prolonged or interrupted sitting and after mixed meal	6.5 hours
Secondary	Changes in plasma glucose concentration from a capillary sample	Changes in plasma glucose concentration will be assessed in response to the prolonged or interrupted sitting and after mixed meal	6.5 hours
Secondary	Blood pressure	Changes in blood pressure will be monitored hourly throughout the 3 hour sitting period and three hour postprandial period	6.5 hours
Secondary	Heart rate	Changes in heart rate will be monitored hourly throughout the 3 hour sitting period and three hour postprandial period	6.5 hours
Secondary	Calf circumference	Calf circumference will be monitored every hour throughout experimental visit to give an indication of venous pooling that may occur as a result of the prolonged sitting and be implicated in the potential decline in vascular function	6.5 hours
Secondary	Physical activity prior to the experimental visits	Participants will wear GENEactiv accelerometers to monitor their physical activity levels before the experimental visit	48 hours prior to each visit
Secondary	Sitting time during the experimental visits	Participants will wear an activPAL accelerometer on their thigh to quantify the amount of time they spend sitting, standing and cycling during the trials	6 hours