Embryo Quality Clinical Trial
Official title:
Group Culture May Affect Human Embryo Quality in Assisted Reproduction Technology
One of the vital factors in achieving pregnancies in assisted reproductive technologies (ART) is maximizing embryo quality, which is significantly influenced by embryo culture conditions. A microwell culture system that facilitates group culture, improves embryonic development. The investigators use a new special culture dish to examine the effect of group culture on embryonic development in vitro. Zygotes derived from sibling oocytes were completely denuded from cumulus cells and spermatozoa, then randomly divided into conventional droplet culture or group culture. Embryos cultured in microwell system (group culture) were compared to conventional droplet culture (one zygote per drop). Relevant parameters are recorded to evaluate the validity of group culture in ART.
Providing optimal culture conditions for the embryos in ART is crucial, due to its high
impact on embryo development and thus on the pregnancy outcome. Several strategies have been
proposed in order to improve embryo culture conditions and clinical pregnancy rates.
Currently, the establishment of autocrine and paracrine communications and embryo-to-embryo
interactions should contribute to the detoxification of the culture medium, facilitating
embryo development and improving the implantation rate. Many studies have provided evidence
related to the impact of group culture condition on the success of the embryonic development
in animals. But the advantage of group culture to improve the embryonic development and
pregnancy outcomes of ART in human remains elusive. Therefore, the investigator aim to
discuss the effectiveness of group culture in ART.
In this study, zygotes derived from sibling oocytes were randomized 1:1 to either the
microwell group culture (experiment group) or conventional droplet culture (control group).
In experiment group, 4 zygotes cultured in a new special microwell (120 μl droplet were
divided into 4 areas) with a corral dish (EMBC-010, LifeGlobal), embryos can communicate with
each other in the same culture environment. In control group, single embryo individually
cultured in a droplet (60 μl). Embryos were cultured in specific desktop three-gas incubators
(COOK) of 37°C, 6% CO2 (carbon dioxide) , 5% O2 and 89% N2, and scored according to the
criteria described in the ESHRE-ALPHA consensus. Consequently, exploitable embryos rate, good
embryo quality, blastocyst formation rate were recorded. Other relevant parameters such as
clinical pregnancy, live-birth or miscarriage were also recorded to evaluate the outcomes.
Proper culture conditions can result in superior embryo viability, this research intends to
discuss whether group culture can improve the embryo quality and clinical outcomes in ART.
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